Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock

The objectives of this study were to estimate genetic parameters for citric acid content (CA) and lactic acid content (LA) in sheep milk and to identify the associated candidate genes in a New Zealand dairy sheep flock. Records from 165 ewes were used. Heritability estimates based on pedigree record...

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Main Authors: An Zongqi, Ana C. Marshall, J. M. D. R. Jayawardana, Mike Weeks, Simon M. Loveday, Warren McNabb, Nicolas Lopez-Villalobos
Format: Article
Language:English
Published: Taylor & Francis Group 2024-12-01
Series:Animal Biotechnology
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Online Access:https://www.tandfonline.com/doi/10.1080/10495398.2024.2379897
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author An Zongqi
Ana C. Marshall
J. M. D. R. Jayawardana
Mike Weeks
Simon M. Loveday
Warren McNabb
Nicolas Lopez-Villalobos
author_facet An Zongqi
Ana C. Marshall
J. M. D. R. Jayawardana
Mike Weeks
Simon M. Loveday
Warren McNabb
Nicolas Lopez-Villalobos
author_sort An Zongqi
collection DOAJ
description The objectives of this study were to estimate genetic parameters for citric acid content (CA) and lactic acid content (LA) in sheep milk and to identify the associated candidate genes in a New Zealand dairy sheep flock. Records from 165 ewes were used. Heritability estimates based on pedigree records for CA and LA were 0.65 and 0.33, respectively. The genetic and phenotypic correlations between CA and LA were strong-moderate and negative. Estimates of genomic heritability for CA and LA were also high (0.85, 0.51) and the genomic correlation between CA and LA was strongly negative (-0.96 ± 0.11). No significant associations were found at the Bonferroni level. However, one intragenic SNP in C1QTNF1 (chromosome 11) was associated with CA, at the chromosomal significance threshold. Another SNP associated with CA was intergenic (chromosome 15). For LA, the most notable SNP was intragenic in CYTH1 (chromosome 11), the other two SNPs were intragenic in MGAT5B and TIMP2 (chromosome 11), and four SNPs were intergenic (chromosomes 1 and 24). The functions of candidate genes indicate that CA and LA could potentially be used as biomarkers for energy balance and clinical mastitis. Further research is recommended to validate the present results.
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spelling doaj-art-824f0c96dd8c42ba8afdd9ccd83555752024-12-31T18:28:13ZengTaylor & Francis GroupAnimal Biotechnology1049-53981532-23782024-12-0135110.1080/10495398.2024.2379897Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flockAn Zongqi0Ana C. Marshall1J. M. D. R. Jayawardana2Mike Weeks3Simon M. Loveday4Warren McNabb5Nicolas Lopez-Villalobos6Sichuan Agricultural University, College of Science and Technology, Farm Animal Genetic Resources Exploration and Innovation Key Laboratory of Sichuan Province, Chengdu, P. R. ChinaSchool of Agriculture and Environment, Massey University, Palmerston North, New ZealandSchool of Agriculture and Environment, Massey University, Palmerston North, New ZealandSmart Foods & Bioproducts Group, AgResearch Ltd, Massey University, Palmerston North, New ZealandThe Riddet Institute, Massey University, Palmerston North, New ZealandThe Riddet Institute, Massey University, Palmerston North, New ZealandSchool of Agriculture and Environment, Massey University, Palmerston North, New ZealandThe objectives of this study were to estimate genetic parameters for citric acid content (CA) and lactic acid content (LA) in sheep milk and to identify the associated candidate genes in a New Zealand dairy sheep flock. Records from 165 ewes were used. Heritability estimates based on pedigree records for CA and LA were 0.65 and 0.33, respectively. The genetic and phenotypic correlations between CA and LA were strong-moderate and negative. Estimates of genomic heritability for CA and LA were also high (0.85, 0.51) and the genomic correlation between CA and LA was strongly negative (-0.96 ± 0.11). No significant associations were found at the Bonferroni level. However, one intragenic SNP in C1QTNF1 (chromosome 11) was associated with CA, at the chromosomal significance threshold. Another SNP associated with CA was intergenic (chromosome 15). For LA, the most notable SNP was intragenic in CYTH1 (chromosome 11), the other two SNPs were intragenic in MGAT5B and TIMP2 (chromosome 11), and four SNPs were intergenic (chromosomes 1 and 24). The functions of candidate genes indicate that CA and LA could potentially be used as biomarkers for energy balance and clinical mastitis. Further research is recommended to validate the present results.https://www.tandfonline.com/doi/10.1080/10495398.2024.2379897Sheep milkcitric acidlactic acidgenome-wide-association study
spellingShingle An Zongqi
Ana C. Marshall
J. M. D. R. Jayawardana
Mike Weeks
Simon M. Loveday
Warren McNabb
Nicolas Lopez-Villalobos
Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock
Animal Biotechnology
Sheep milk
citric acid
lactic acid
genome-wide-association study
title Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock
title_full Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock
title_fullStr Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock
title_full_unstemmed Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock
title_short Genome-wide association studies for citric and lactic acids in dairy sheep milk in a New Zealand flock
title_sort genome wide association studies for citric and lactic acids in dairy sheep milk in a new zealand flock
topic Sheep milk
citric acid
lactic acid
genome-wide-association study
url https://www.tandfonline.com/doi/10.1080/10495398.2024.2379897
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