Hidden route of protein damage through oxygen-confined photooxidation
Abstract Oxidative modifications can disrupt protein folds and functions, and are strongly associated with human aging and diseases. Conventional oxidation pathways typically involve the free diffusion of reactive oxygen species (ROS), which primarily attack the protein surface. Yet, it remains uncl...
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| Format: | Article |
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Nature Portfolio
2024-12-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-024-55168-z |
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| author | Seoyoon Kim Eojin Kim Mingyu Park Seong Ho Kim Byung-Gyu Kim Seungjin Na Victor W. Sadongo W. C. Bhashini Wijesinghe Yu-Gon Eom Gwangsu Yoon Hannah Jeong Eunhye Hwang Chaiheon Lee Kyungjae Myung Chae Un Kim Jeong-Mo Choi Seung Kyu Min Tae-Hyuk Kwon Duyoung Min |
| author_facet | Seoyoon Kim Eojin Kim Mingyu Park Seong Ho Kim Byung-Gyu Kim Seungjin Na Victor W. Sadongo W. C. Bhashini Wijesinghe Yu-Gon Eom Gwangsu Yoon Hannah Jeong Eunhye Hwang Chaiheon Lee Kyungjae Myung Chae Un Kim Jeong-Mo Choi Seung Kyu Min Tae-Hyuk Kwon Duyoung Min |
| author_sort | Seoyoon Kim |
| collection | DOAJ |
| description | Abstract Oxidative modifications can disrupt protein folds and functions, and are strongly associated with human aging and diseases. Conventional oxidation pathways typically involve the free diffusion of reactive oxygen species (ROS), which primarily attack the protein surface. Yet, it remains unclear whether and how internal protein folds capable of trapping oxygen (O2) contribute to oxidative damage. Here, we report a hidden pathway of protein damage, which we refer to as O2-confined photooxidation. In this process, O2 is captured in protein cavities and subsequently converted into multiple ROS, primarily mediated by tryptophan residues under blue light irradiation. The generated ROS then attack the protein interior through constrained diffusion, causing protein damage. The effects of this photooxidative reaction appear to be extensive, impacting a wide range of cellular proteins, as supported by whole-cell proteomic analysis. This photooxidative mechanism may represent a latent oxidation pathway in human tissues directly exposed to visible light, such as skin and eyes. |
| format | Article |
| id | doaj-art-82444e44a71940509a8247999224af02 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-82444e44a71940509a8247999224af022025-01-05T12:35:30ZengNature PortfolioNature Communications2041-17232024-12-0115111910.1038/s41467-024-55168-zHidden route of protein damage through oxygen-confined photooxidationSeoyoon Kim0Eojin Kim1Mingyu Park2Seong Ho Kim3Byung-Gyu Kim4Seungjin Na5Victor W. Sadongo6W. C. Bhashini Wijesinghe7Yu-Gon Eom8Gwangsu Yoon9Hannah Jeong10Eunhye Hwang11Chaiheon Lee12Kyungjae Myung13Chae Un Kim14Jeong-Mo Choi15Seung Kyu Min16Tae-Hyuk Kwon17Duyoung Min18Department of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Ulsan National Institute of Science and TechnologyCenter for Genomic Integrity, Institute for Basic ScienceDigital Omics Research Center, Korea Basic Science InstituteDepartment of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Pusan National UniversityDepartment of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Physics, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Ulsan National Institute of Science and TechnologyCenter for Genomic Integrity, Institute for Basic ScienceDepartment of Physics, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Pusan National UniversityDepartment of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Ulsan National Institute of Science and TechnologyDepartment of Chemistry, Ulsan National Institute of Science and TechnologyAbstract Oxidative modifications can disrupt protein folds and functions, and are strongly associated with human aging and diseases. Conventional oxidation pathways typically involve the free diffusion of reactive oxygen species (ROS), which primarily attack the protein surface. Yet, it remains unclear whether and how internal protein folds capable of trapping oxygen (O2) contribute to oxidative damage. Here, we report a hidden pathway of protein damage, which we refer to as O2-confined photooxidation. In this process, O2 is captured in protein cavities and subsequently converted into multiple ROS, primarily mediated by tryptophan residues under blue light irradiation. The generated ROS then attack the protein interior through constrained diffusion, causing protein damage. The effects of this photooxidative reaction appear to be extensive, impacting a wide range of cellular proteins, as supported by whole-cell proteomic analysis. This photooxidative mechanism may represent a latent oxidation pathway in human tissues directly exposed to visible light, such as skin and eyes.https://doi.org/10.1038/s41467-024-55168-z |
| spellingShingle | Seoyoon Kim Eojin Kim Mingyu Park Seong Ho Kim Byung-Gyu Kim Seungjin Na Victor W. Sadongo W. C. Bhashini Wijesinghe Yu-Gon Eom Gwangsu Yoon Hannah Jeong Eunhye Hwang Chaiheon Lee Kyungjae Myung Chae Un Kim Jeong-Mo Choi Seung Kyu Min Tae-Hyuk Kwon Duyoung Min Hidden route of protein damage through oxygen-confined photooxidation Nature Communications |
| title | Hidden route of protein damage through oxygen-confined photooxidation |
| title_full | Hidden route of protein damage through oxygen-confined photooxidation |
| title_fullStr | Hidden route of protein damage through oxygen-confined photooxidation |
| title_full_unstemmed | Hidden route of protein damage through oxygen-confined photooxidation |
| title_short | Hidden route of protein damage through oxygen-confined photooxidation |
| title_sort | hidden route of protein damage through oxygen confined photooxidation |
| url | https://doi.org/10.1038/s41467-024-55168-z |
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