Zinc finger proteins facilitate adaptation of a global insect pest to climate change
Abstract Background Global climate change significantly impacts ecosystems, particularly through temperature fluctuations that affect insect physiology and behavior. As poikilotherms, insect pests such as the globally devastating diamondback moth (DBM), Plutella xylostella, are especially vulnerable...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-12-01
|
| Series: | BMC Biology |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12915-024-02109-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841559076051353600 |
|---|---|
| author | Tianpu Li Jiao Guo Guilei Hu Fang Cao Haiyin Su Mengdi Shen Huimin Wang Minsheng You Yuanyuan Liu Geoff M. Gurr Shijun You |
| author_facet | Tianpu Li Jiao Guo Guilei Hu Fang Cao Haiyin Su Mengdi Shen Huimin Wang Minsheng You Yuanyuan Liu Geoff M. Gurr Shijun You |
| author_sort | Tianpu Li |
| collection | DOAJ |
| description | Abstract Background Global climate change significantly impacts ecosystems, particularly through temperature fluctuations that affect insect physiology and behavior. As poikilotherms, insect pests such as the globally devastating diamondback moth (DBM), Plutella xylostella, are especially vulnerable to rising temperatures and extreme heat events, necessitating effective adaptive mechanisms. Results Here we demonstrate the roles of zinc finger proteins (ZFPs) in mediating thermal adaptability in DBM. We utilized a comprehensive approach involving cloning and bioinformatics analysis of three ZFPs, PxZNF568, PxZNF93, and PxZNF266, measurement of their expression levels in hot-evolved and control strains, and assessment of catalase enzymatic activity and total antioxidant capacity. We also employed CRISPR/Cas9 technology to create five stable homozygous knockout strains to elucidate ZFP functions in high-temperature tolerance. Survival rates under high-temperature stress and the critical thermal maxima (CTMax) of the knockout strains were significantly lower than the wild-type strain, and exhibited marked decreases in antioxidant capacity. Conclusion Findings reveal the importance of ZFPs in thermal adaptability of DBM, contributing critical insights for future pest management strategies in the context of a warming climate and laying the foundation for further exploration of ZFP functionality in agricultural pest control. |
| format | Article |
| id | doaj-art-8203d980337c427a953b5787b4ca61bb |
| institution | Kabale University |
| issn | 1741-7007 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Biology |
| spelling | doaj-art-8203d980337c427a953b5787b4ca61bb2025-01-05T12:46:14ZengBMCBMC Biology1741-70072024-12-0122111710.1186/s12915-024-02109-3Zinc finger proteins facilitate adaptation of a global insect pest to climate changeTianpu Li0Jiao Guo1Guilei Hu2Fang Cao3Haiyin Su4Mengdi Shen5Huimin Wang6Minsheng You7Yuanyuan Liu8Geoff M. Gurr9Shijun You10State Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityHaixia Lnstitute of Science and Technology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityState Key Laboratory for Ecological Pest Control of Fujian and Taiwan Crops, Institute of Applied Ecology, Fujian Agriculture and Forestry UniversityAbstract Background Global climate change significantly impacts ecosystems, particularly through temperature fluctuations that affect insect physiology and behavior. As poikilotherms, insect pests such as the globally devastating diamondback moth (DBM), Plutella xylostella, are especially vulnerable to rising temperatures and extreme heat events, necessitating effective adaptive mechanisms. Results Here we demonstrate the roles of zinc finger proteins (ZFPs) in mediating thermal adaptability in DBM. We utilized a comprehensive approach involving cloning and bioinformatics analysis of three ZFPs, PxZNF568, PxZNF93, and PxZNF266, measurement of their expression levels in hot-evolved and control strains, and assessment of catalase enzymatic activity and total antioxidant capacity. We also employed CRISPR/Cas9 technology to create five stable homozygous knockout strains to elucidate ZFP functions in high-temperature tolerance. Survival rates under high-temperature stress and the critical thermal maxima (CTMax) of the knockout strains were significantly lower than the wild-type strain, and exhibited marked decreases in antioxidant capacity. Conclusion Findings reveal the importance of ZFPs in thermal adaptability of DBM, contributing critical insights for future pest management strategies in the context of a warming climate and laying the foundation for further exploration of ZFP functionality in agricultural pest control.https://doi.org/10.1186/s12915-024-02109-3Plutella xylostellaCRISPR/Cas9Zinc finger proteinsTemperature adaptability |
| spellingShingle | Tianpu Li Jiao Guo Guilei Hu Fang Cao Haiyin Su Mengdi Shen Huimin Wang Minsheng You Yuanyuan Liu Geoff M. Gurr Shijun You Zinc finger proteins facilitate adaptation of a global insect pest to climate change BMC Biology Plutella xylostella CRISPR/Cas9 Zinc finger proteins Temperature adaptability |
| title | Zinc finger proteins facilitate adaptation of a global insect pest to climate change |
| title_full | Zinc finger proteins facilitate adaptation of a global insect pest to climate change |
| title_fullStr | Zinc finger proteins facilitate adaptation of a global insect pest to climate change |
| title_full_unstemmed | Zinc finger proteins facilitate adaptation of a global insect pest to climate change |
| title_short | Zinc finger proteins facilitate adaptation of a global insect pest to climate change |
| title_sort | zinc finger proteins facilitate adaptation of a global insect pest to climate change |
| topic | Plutella xylostella CRISPR/Cas9 Zinc finger proteins Temperature adaptability |
| url | https://doi.org/10.1186/s12915-024-02109-3 |
| work_keys_str_mv | AT tianpuli zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT jiaoguo zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT guileihu zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT fangcao zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT haiyinsu zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT mengdishen zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT huiminwang zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT minshengyou zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT yuanyuanliu zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT geoffmgurr zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange AT shijunyou zincfingerproteinsfacilitateadaptationofaglobalinsectpesttoclimatechange |