Digital light processing printing of non-modified protein-only compositions
This study explores the utilization of digital light processing (DLP) printing to fabricate complex structures using native gelatin as the sole structural component for applications in biological implants. Unlike approaches relying on synthetic materials or chemically modified biopolymers, this rese...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-02-01
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| Series: | Materials Today Bio |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590006424004459 |
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| author | Ayelet Bunin Orit Harari-Steinberg Doron Kam Tatyana Kuperman Moran Friedman-Gohas Bruria Shalmon Liraz Larush Shay I. Duvdevani Shlomo Magdassi |
| author_facet | Ayelet Bunin Orit Harari-Steinberg Doron Kam Tatyana Kuperman Moran Friedman-Gohas Bruria Shalmon Liraz Larush Shay I. Duvdevani Shlomo Magdassi |
| author_sort | Ayelet Bunin |
| collection | DOAJ |
| description | This study explores the utilization of digital light processing (DLP) printing to fabricate complex structures using native gelatin as the sole structural component for applications in biological implants. Unlike approaches relying on synthetic materials or chemically modified biopolymers, this research harnesses the inherent properties of gelatin to create biocompatible structures. The printing process is based on a crosslinking mechanism using a di-tyrosine formation initiated by visible light irradiation. Formulations containing gelatin were found to be printable at the maximum documented concentration of 30 wt%, thus allowing the fabrication of overhanging objects and open embedded. Cell adhesion and growth onto and within the gelatin-based 3D constructs were evaluated by examining two implant fabrication techniques: (1) cell seeding onto the printed scaffold and (2) printing compositions that contain cells (cell-laden). The preliminary biological experiments indicate that both the cell-seeding and cell-laden strategies enable making 3D cultures of chondrocytes within the gelatin constructs. The mechanical properties of the gelatin scaffolds have a compressive modulus akin to soft tissues, thus enabling the growth and proliferation of cells, and later degrade as the cells differentiate and form a grown cartilage. This study underscores the potential of utilizing non-modified protein-only bioinks in DLP printing to produce intricate 3D objects with high fidelity, paving the way for advancements in regenerative tissue engineering. |
| format | Article |
| id | doaj-art-818b1bfb790743099d8b3f0963ee8e2b |
| institution | Kabale University |
| issn | 2590-0064 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Materials Today Bio |
| spelling | doaj-art-818b1bfb790743099d8b3f0963ee8e2b2025-01-17T04:52:03ZengElsevierMaterials Today Bio2590-00642025-02-0130101384Digital light processing printing of non-modified protein-only compositionsAyelet Bunin0Orit Harari-Steinberg1Doron Kam2Tatyana Kuperman3Moran Friedman-Gohas4Bruria Shalmon5Liraz Larush6Shay I. Duvdevani7Shlomo Magdassi8Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 9190401, IsraelTissue Engineering Research Laboratory, Sheba Medical Center, Tel Hashomer, Ramat-Gan, IsraelInstitute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 9190401, IsraelTissue Engineering Research Laboratory, Sheba Medical Center, Tel Hashomer, Ramat-Gan, IsraelTissue Engineering Research Laboratory, Sheba Medical Center, Tel Hashomer, Ramat-Gan, IsraelTissue Engineering Research Laboratory, Sheba Medical Center, Tel Hashomer, Ramat-Gan, Israel; Department of pathology, Sheba Medical Center, Tel Hashomer, Ramat-Gan, Israel; Sackler Faculty of Medicine, Tel Aviv University, IsraelInstitute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 9190401, IsraelTissue Engineering Research Laboratory, Sheba Medical Center, Tel Hashomer, Ramat-Gan, Israel; Department of Otorhinolaryngology, Head and Neck Surgery, Sheba Medical Center, Israel; Sackler Faculty of Medicine, Tel Aviv University, Israel; Corresponding author. Tissue Engineering Research Laboratory, Sheba Medical Center, Tel Hashomer, Ramat-Gan, Israel.Institute of Chemistry and Center for Nanoscience and Nanotechnology, The Hebrew University of Jerusalem, Jerusalem, 9190401, Israel; Corresponding author.This study explores the utilization of digital light processing (DLP) printing to fabricate complex structures using native gelatin as the sole structural component for applications in biological implants. Unlike approaches relying on synthetic materials or chemically modified biopolymers, this research harnesses the inherent properties of gelatin to create biocompatible structures. The printing process is based on a crosslinking mechanism using a di-tyrosine formation initiated by visible light irradiation. Formulations containing gelatin were found to be printable at the maximum documented concentration of 30 wt%, thus allowing the fabrication of overhanging objects and open embedded. Cell adhesion and growth onto and within the gelatin-based 3D constructs were evaluated by examining two implant fabrication techniques: (1) cell seeding onto the printed scaffold and (2) printing compositions that contain cells (cell-laden). The preliminary biological experiments indicate that both the cell-seeding and cell-laden strategies enable making 3D cultures of chondrocytes within the gelatin constructs. The mechanical properties of the gelatin scaffolds have a compressive modulus akin to soft tissues, thus enabling the growth and proliferation of cells, and later degrade as the cells differentiate and form a grown cartilage. This study underscores the potential of utilizing non-modified protein-only bioinks in DLP printing to produce intricate 3D objects with high fidelity, paving the way for advancements in regenerative tissue engineering.http://www.sciencedirect.com/science/article/pii/S25900064240044593D printingDigital light processing (DLP)Non-modifieddi-tyrosineCell-laden |
| spellingShingle | Ayelet Bunin Orit Harari-Steinberg Doron Kam Tatyana Kuperman Moran Friedman-Gohas Bruria Shalmon Liraz Larush Shay I. Duvdevani Shlomo Magdassi Digital light processing printing of non-modified protein-only compositions Materials Today Bio 3D printing Digital light processing (DLP) Non-modified di-tyrosine Cell-laden |
| title | Digital light processing printing of non-modified protein-only compositions |
| title_full | Digital light processing printing of non-modified protein-only compositions |
| title_fullStr | Digital light processing printing of non-modified protein-only compositions |
| title_full_unstemmed | Digital light processing printing of non-modified protein-only compositions |
| title_short | Digital light processing printing of non-modified protein-only compositions |
| title_sort | digital light processing printing of non modified protein only compositions |
| topic | 3D printing Digital light processing (DLP) Non-modified di-tyrosine Cell-laden |
| url | http://www.sciencedirect.com/science/article/pii/S2590006424004459 |
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