Learned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expression
Abstract Psychological distress, including anxiety or mood disorders, emanates from the onset of chronic/unpredictable stressful events. Symptoms in the form of maladaptive behaviors are learned and difficult to treat. While the origin of stress-induced disorders seems to be where learning and stres...
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Nature Portfolio
2024-12-01
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| Online Access: | https://doi.org/10.1038/s41598-024-81590-w |
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| author | Jazmine D. W. Yaeger Justin K. Achua Clarissa D. Booth Delan Khalid Megan M. John Leighton J. Ledesma Trent L. Greschke Ashley M. Potter Chase B. Howe Kevin T. Krupp Justin P. Smith Patrick J. Ronan Cliff H. Summers |
| author_facet | Jazmine D. W. Yaeger Justin K. Achua Clarissa D. Booth Delan Khalid Megan M. John Leighton J. Ledesma Trent L. Greschke Ashley M. Potter Chase B. Howe Kevin T. Krupp Justin P. Smith Patrick J. Ronan Cliff H. Summers |
| author_sort | Jazmine D. W. Yaeger |
| collection | DOAJ |
| description | Abstract Psychological distress, including anxiety or mood disorders, emanates from the onset of chronic/unpredictable stressful events. Symptoms in the form of maladaptive behaviors are learned and difficult to treat. While the origin of stress-induced disorders seems to be where learning and stress intersect, this relationship and molecular pathways involved remain largely unresolved. The hippocampus, studied for its role in learning, is divided into regions that designate the passage of neuronal signaling during memory formation, including dentate gyrus (DG), CA3, CA2, and CA1. Inputs into these hippocampal subregions, like those from hypothalamic orexinergic neurons, may modify learning outcomes. We have previously shown the orexin system to balance stress states, where receptor subtypes prompt opposing actions on behavior. Here, we explore the connection between hippocampal orexin receptors and learning during stress. In a social stress/learning paradigm separating mice into stress resilient and vulnerable populations, hippocampal Orx1R and Orx2R transcription is regulated in a phenotype-dependent fashion. We further identified Orx1R as highly expressed in the hilus of DG, while Orx2R is abundant in CA2. Finally, we designed an experiment where mice were provided prior exposure to a stressful environment, which ultimately modified behavior, as well as transcription of hippocampal orexin receptors. |
| format | Article |
| id | doaj-art-8152641d1edb4b28accce40bd91d753c |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-8152641d1edb4b28accce40bd91d753c2025-01-05T12:29:55ZengNature PortfolioScientific Reports2045-23222024-12-0114111710.1038/s41598-024-81590-wLearned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expressionJazmine D. W. Yaeger0Justin K. Achua1Clarissa D. Booth2Delan Khalid3Megan M. John4Leighton J. Ledesma5Trent L. Greschke6Ashley M. Potter7Chase B. Howe8Kevin T. Krupp9Justin P. Smith10Patrick J. Ronan11Cliff H. Summers12Cellular Therapies and Stem Cell Biology Group, Sanford ResearchDivision of Urology, University of Colorado School of Medicine, Anschutz Medical CampusPediatrics and Rare Diseases Group, Sanford ResearchSchool of Medicine, BMP, University of PittsburghDepartment of Biology, University of South DakotaDepartment of Biology, University of South DakotaDepartment of Biology, University of South DakotaDepartment of Clinical Sciences, Colorado State UniversityNeuroscience Group, Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South DakotaDepartment of Biology, University of South DakotaAdvanced Analytics GroupNeuroscience Group, Division of Basic Biomedical Sciences, Sanford School of Medicine, University of South DakotaDepartment of Biology, University of South DakotaAbstract Psychological distress, including anxiety or mood disorders, emanates from the onset of chronic/unpredictable stressful events. Symptoms in the form of maladaptive behaviors are learned and difficult to treat. While the origin of stress-induced disorders seems to be where learning and stress intersect, this relationship and molecular pathways involved remain largely unresolved. The hippocampus, studied for its role in learning, is divided into regions that designate the passage of neuronal signaling during memory formation, including dentate gyrus (DG), CA3, CA2, and CA1. Inputs into these hippocampal subregions, like those from hypothalamic orexinergic neurons, may modify learning outcomes. We have previously shown the orexin system to balance stress states, where receptor subtypes prompt opposing actions on behavior. Here, we explore the connection between hippocampal orexin receptors and learning during stress. In a social stress/learning paradigm separating mice into stress resilient and vulnerable populations, hippocampal Orx1R and Orx2R transcription is regulated in a phenotype-dependent fashion. We further identified Orx1R as highly expressed in the hilus of DG, while Orx2R is abundant in CA2. Finally, we designed an experiment where mice were provided prior exposure to a stressful environment, which ultimately modified behavior, as well as transcription of hippocampal orexin receptors.https://doi.org/10.1038/s41598-024-81590-wAmygdalaFamiliarityGene expressionHippocampusOrexin receptorsSpatial learning |
| spellingShingle | Jazmine D. W. Yaeger Justin K. Achua Clarissa D. Booth Delan Khalid Megan M. John Leighton J. Ledesma Trent L. Greschke Ashley M. Potter Chase B. Howe Kevin T. Krupp Justin P. Smith Patrick J. Ronan Cliff H. Summers Learned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expression Scientific Reports Amygdala Familiarity Gene expression Hippocampus Orexin receptors Spatial learning |
| title | Learned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expression |
| title_full | Learned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expression |
| title_fullStr | Learned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expression |
| title_full_unstemmed | Learned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expression |
| title_short | Learned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expression |
| title_sort | learned phenotypes emerge during social stress modifying hippocampal orexin receptor gene expression |
| topic | Amygdala Familiarity Gene expression Hippocampus Orexin receptors Spatial learning |
| url | https://doi.org/10.1038/s41598-024-81590-w |
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