Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout Mice
Infection with the protozoan Trypanosoma cruzi causes Chagas disease and consequently leads to severe inflammatory heart condition; however, the mechanisms driving this inflammatory response have not been completely elucidated. Nitric oxide (NO) is a key mediator of parasite killing in T. cruzi-infe...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2019/5091630 |
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| author | Carolina Panis Vanessa Jacob Victorino Vera Lúcia Hideko Tatakihara Rubens Cecchini Luiz Vicente Rizzo Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogatta Marli Cardoso Martins-Pinge Phileno Pinge-Filho |
| author_facet | Carolina Panis Vanessa Jacob Victorino Vera Lúcia Hideko Tatakihara Rubens Cecchini Luiz Vicente Rizzo Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogatta Marli Cardoso Martins-Pinge Phileno Pinge-Filho |
| author_sort | Carolina Panis |
| collection | DOAJ |
| description | Infection with the protozoan Trypanosoma cruzi causes Chagas disease and consequently leads to severe inflammatory heart condition; however, the mechanisms driving this inflammatory response have not been completely elucidated. Nitric oxide (NO) is a key mediator of parasite killing in T. cruzi-infected mice, and previous studies have suggested that leukotrienes (LTs) essentially regulate the NO activity in the heart. We used infected 5-lipoxygenase-deficient mice (5-LO−/−) to explore the participation of nitric oxide synthase isoforms, inducible (iNOS) and constitutive (cNOS), in heart injury, cytokine profile, and oxidative stress during the early stage of T. cruzi infection. Our evidence suggests that the cNOS of the host is involved in the resistance of 5-LO−/− mice during T. cruzi infection. iNOS inhibition generated a remarkable increase in T. cruzi infection in the blood and heart of mice, whereas cNOS inhibition reduced cardiac parasitism (amastigote nests). Furthermore, this inhibition associates with a higher IFN-γ production and lower lipid peroxidation status. These data provide a better understanding about the influence of NO-interfering therapies for the inflammatory response toward T. cruzi infection. |
| format | Article |
| id | doaj-art-81048a03a4b84c0798e733856bde11e5 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-81048a03a4b84c0798e733856bde11e52025-02-03T05:53:02ZengWileyMediators of Inflammation0962-93511466-18612019-01-01201910.1155/2019/50916305091630Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout MiceCarolina Panis0Vanessa Jacob Victorino1Vera Lúcia Hideko Tatakihara2Rubens Cecchini3Luiz Vicente Rizzo4Lucy Megumi Yamauchi5Sueli Fumie Yamada-Ogatta6Marli Cardoso Martins-Pinge7Phileno Pinge-Filho8Laboratório de Mediadores Inflamatórios, Universidade Estadual do Oeste do Paraná, Francisco Beltrão, Paraná 85605-010, BrazilLaboratório de Mediadores Inflamatórios, Universidade Estadual do Oeste do Paraná, Francisco Beltrão, Paraná 85605-010, BrazilLaboratório de Imunopatologia Experimental, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, 86051-970 Paraná, BrazilLaboratório de Patofisiologia e Radicais Livres, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná 86051-970, BrazilHospital Israelita Albert Einstein, Avenida Albert Einstein 627-701, Subsolo Bloco A., 05651-901 São Paulo, São Paulo, BrazilDepartamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná 86051-970, BrazilDepartamento de Microbiologia, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná 86051-970, BrazilDepartamento de Ciências Fisiológicas, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, Paraná 86051-970, BrazilLaboratório de Imunopatologia Experimental, Centro de Ciências Biológicas, Universidade Estadual de Londrina, Londrina, 86051-970 Paraná, BrazilInfection with the protozoan Trypanosoma cruzi causes Chagas disease and consequently leads to severe inflammatory heart condition; however, the mechanisms driving this inflammatory response have not been completely elucidated. Nitric oxide (NO) is a key mediator of parasite killing in T. cruzi-infected mice, and previous studies have suggested that leukotrienes (LTs) essentially regulate the NO activity in the heart. We used infected 5-lipoxygenase-deficient mice (5-LO−/−) to explore the participation of nitric oxide synthase isoforms, inducible (iNOS) and constitutive (cNOS), in heart injury, cytokine profile, and oxidative stress during the early stage of T. cruzi infection. Our evidence suggests that the cNOS of the host is involved in the resistance of 5-LO−/− mice during T. cruzi infection. iNOS inhibition generated a remarkable increase in T. cruzi infection in the blood and heart of mice, whereas cNOS inhibition reduced cardiac parasitism (amastigote nests). Furthermore, this inhibition associates with a higher IFN-γ production and lower lipid peroxidation status. These data provide a better understanding about the influence of NO-interfering therapies for the inflammatory response toward T. cruzi infection.http://dx.doi.org/10.1155/2019/5091630 |
| spellingShingle | Carolina Panis Vanessa Jacob Victorino Vera Lúcia Hideko Tatakihara Rubens Cecchini Luiz Vicente Rizzo Lucy Megumi Yamauchi Sueli Fumie Yamada-Ogatta Marli Cardoso Martins-Pinge Phileno Pinge-Filho Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout Mice Mediators of Inflammation |
| title | Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout Mice |
| title_full | Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout Mice |
| title_fullStr | Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout Mice |
| title_full_unstemmed | Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout Mice |
| title_short | Differences in cNOS/iNOS Activity during Resistance to Trypanosoma cruzi Infection in 5-Lipoxygenase Knockout Mice |
| title_sort | differences in cnos inos activity during resistance to trypanosoma cruzi infection in 5 lipoxygenase knockout mice |
| url | http://dx.doi.org/10.1155/2019/5091630 |
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