CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B
Osteosarcoma (OS) is a primary malignant bone tumor in children and young adults because of its intertumoral heterogeneity and absence of molecular targets. This study aimed to elucidate the role of circular RNA in the pathogenesis of OS. Using bioinformatics analysis and OS clinical samples, we fou...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-11-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024137759 |
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| author | Fei Li Jiaqing Wei Xiaoxuan Ren Yawei Hao Shuaishuai Tian Lizhi Li |
| author_facet | Fei Li Jiaqing Wei Xiaoxuan Ren Yawei Hao Shuaishuai Tian Lizhi Li |
| author_sort | Fei Li |
| collection | DOAJ |
| description | Osteosarcoma (OS) is a primary malignant bone tumor in children and young adults because of its intertumoral heterogeneity and absence of molecular targets. This study aimed to elucidate the role of circular RNA in the pathogenesis of OS. Using bioinformatics analysis and OS clinical samples, we found that hsa_circ_0029634 formed during the splicing process of Zinc finger MYM-type containing 2 (circZMYM2) was highly expressed in OS tissues. Nuclear cap-binding protein subunit 2 regulated circZMYM2 bio-generation by binding to flanking sequences of ZMYM2 transcripts. In addition, knockdown circZMYM2 inhibited cell growth and metastasis in OS cell lines in vitro and inhibited tumor growth in vivo. Mechanistically, circZMYM2 competitively bound to FXR1 to regulate the translation of NACA and ARPC1B. CircZMYM2 may be a crucial regulator of OS tumorigenesis and a potential diagnostic and therapeutic biomarker for OS patients. |
| format | Article |
| id | doaj-art-80c96ad38ef24fdf83e0eb5a18770d3d |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-80c96ad38ef24fdf83e0eb5a18770d3d2024-11-30T07:11:18ZengElsevierHeliyon2405-84402024-11-011022e37744CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1BFei Li0Jiaqing Wei1Xiaoxuan Ren2Yawei Hao3Shuaishuai Tian4Lizhi Li5Department of Orthopaedics, Second Hospital of Shanxi Medical University, No. 382, Wuyi Road, Taiyuan, 030001, PR ChinaDepartment of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, 030001, PR ChinaDepartment of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, 030001, PR ChinaDepartment of Orthopaedics, Second Hospital of Shanxi Medical University, No. 382, Wuyi Road, Taiyuan, 030001, PR ChinaDepartment of Biochemistry and Molecular Biology, Shanxi Medical University, Taiyuan, 030001, PR ChinaDepartment of Orthopaedics, Second Hospital of Shanxi Medical University, No. 382, Wuyi Road, Taiyuan, 030001, PR China; Corresponding author.Osteosarcoma (OS) is a primary malignant bone tumor in children and young adults because of its intertumoral heterogeneity and absence of molecular targets. This study aimed to elucidate the role of circular RNA in the pathogenesis of OS. Using bioinformatics analysis and OS clinical samples, we found that hsa_circ_0029634 formed during the splicing process of Zinc finger MYM-type containing 2 (circZMYM2) was highly expressed in OS tissues. Nuclear cap-binding protein subunit 2 regulated circZMYM2 bio-generation by binding to flanking sequences of ZMYM2 transcripts. In addition, knockdown circZMYM2 inhibited cell growth and metastasis in OS cell lines in vitro and inhibited tumor growth in vivo. Mechanistically, circZMYM2 competitively bound to FXR1 to regulate the translation of NACA and ARPC1B. CircZMYM2 may be a crucial regulator of OS tumorigenesis and a potential diagnostic and therapeutic biomarker for OS patients.http://www.sciencedirect.com/science/article/pii/S2405844024137759OScircZMYM2FXR1NACAARPC1B |
| spellingShingle | Fei Li Jiaqing Wei Xiaoxuan Ren Yawei Hao Shuaishuai Tian Lizhi Li CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B Heliyon OS circZMYM2 FXR1 NACA ARPC1B |
| title | CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B |
| title_full | CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B |
| title_fullStr | CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B |
| title_full_unstemmed | CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B |
| title_short | CircZMYM2 plays a pivotal role in osteosarcoma by regulating the translation of NACA and ARPC1B |
| title_sort | circzmym2 plays a pivotal role in osteosarcoma by regulating the translation of naca and arpc1b |
| topic | OS circZMYM2 FXR1 NACA ARPC1B |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024137759 |
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