Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa
Abstract Wild relatives of wheat are valuable sources for enhancing the genetic diversity of common wheat. Aegilops comosa, an annual diploid species with an MM genome constitution, possesses numerous agronomically valuable traits that can be exploited for wheat improvement. In this study, we report...
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Nature Portfolio
2024-12-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-024-04346-1 |
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| author | Hongna Li Shams ur Rehman Rui Song Liang Qiao Xiaohua Hao Jing Zhang Kairong Li Lifeng Hou Wanyi Hu Le Wang Shisheng Chen |
| author_facet | Hongna Li Shams ur Rehman Rui Song Liang Qiao Xiaohua Hao Jing Zhang Kairong Li Lifeng Hou Wanyi Hu Le Wang Shisheng Chen |
| author_sort | Hongna Li |
| collection | DOAJ |
| description | Abstract Wild relatives of wheat are valuable sources for enhancing the genetic diversity of common wheat. Aegilops comosa, an annual diploid species with an MM genome constitution, possesses numerous agronomically valuable traits that can be exploited for wheat improvement. In this study, we report a chromosome-level genome assembly of Ae. comosa accession PI 551049, generated using PacBio high-fidelity (HiFi) reads and high-throughput chromosome conformation capture (Hi-C) data. The assembly spans 4.47 Gb, featuring a contig N50 of 23.59 Mb and a scaffold N50 of 619.05 Mb. A total of 39,057 gene models were annotated through a combination of homoeologous proteins, Iso-seq, and RNA-seq data. Comparative genome analysis revealed a terminal intrachromosomal translocation on chromosome 2 M in Ae. comosa. This newly constructed reference genome of Ae. comosa will serve as an important genomic resource for comparative genomic studies and the cloning of agriculturally important genes. |
| format | Article |
| id | doaj-art-80884f25f8c74d63b5b40ed24f1a284c |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-80884f25f8c74d63b5b40ed24f1a284c2025-01-05T12:08:16ZengNature PortfolioScientific Data2052-44632024-12-011111910.1038/s41597-024-04346-1Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosaHongna Li0Shams ur Rehman1Rui Song2Liang Qiao3Xiaohua Hao4Jing Zhang5Kairong Li6Lifeng Hou7Wanyi Hu8Le Wang9Shisheng Chen10State Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangCollege of Plant Science, Jilin UniversityState Key Laboratory of Wheat Improvement, Peking University Institute of Advanced Agricultural Sciences, Shandong Laboratory of Advanced Agriculture Sciences in WeifangAbstract Wild relatives of wheat are valuable sources for enhancing the genetic diversity of common wheat. Aegilops comosa, an annual diploid species with an MM genome constitution, possesses numerous agronomically valuable traits that can be exploited for wheat improvement. In this study, we report a chromosome-level genome assembly of Ae. comosa accession PI 551049, generated using PacBio high-fidelity (HiFi) reads and high-throughput chromosome conformation capture (Hi-C) data. The assembly spans 4.47 Gb, featuring a contig N50 of 23.59 Mb and a scaffold N50 of 619.05 Mb. A total of 39,057 gene models were annotated through a combination of homoeologous proteins, Iso-seq, and RNA-seq data. Comparative genome analysis revealed a terminal intrachromosomal translocation on chromosome 2 M in Ae. comosa. This newly constructed reference genome of Ae. comosa will serve as an important genomic resource for comparative genomic studies and the cloning of agriculturally important genes.https://doi.org/10.1038/s41597-024-04346-1 |
| spellingShingle | Hongna Li Shams ur Rehman Rui Song Liang Qiao Xiaohua Hao Jing Zhang Kairong Li Lifeng Hou Wanyi Hu Le Wang Shisheng Chen Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa Scientific Data |
| title | Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa |
| title_full | Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa |
| title_fullStr | Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa |
| title_full_unstemmed | Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa |
| title_short | Chromosome-scale assembly and annotation of the wild wheat relative Aegilops comosa |
| title_sort | chromosome scale assembly and annotation of the wild wheat relative aegilops comosa |
| url | https://doi.org/10.1038/s41597-024-04346-1 |
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