Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae)
Abstract Objective The ghost crab Ocypode stimpsoni (Decapoda) is designated as a protected marine species in Korea due to its declining population. In this study, we successfully identified 17 microsatellite markers for O. stimpsoni through next-generation sequencing. Results Out of the 63 loci exa...
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| Language: | English |
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BMC
2024-12-01
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| Series: | BMC Research Notes |
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| Online Access: | https://doi.org/10.1186/s13104-024-07049-3 |
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| author | Ha Yeun Song Young Ji Choi Dea-Sung Lee Hyun Ki Choi Kyung Min Choi Kang-Yeol Yu Namki Cho Seungki Lee |
| author_facet | Ha Yeun Song Young Ji Choi Dea-Sung Lee Hyun Ki Choi Kyung Min Choi Kang-Yeol Yu Namki Cho Seungki Lee |
| author_sort | Ha Yeun Song |
| collection | DOAJ |
| description | Abstract Objective The ghost crab Ocypode stimpsoni (Decapoda) is designated as a protected marine species in Korea due to its declining population. In this study, we successfully identified 17 microsatellite markers for O. stimpsoni through next-generation sequencing. Results Out of the 63 loci examined, 26 were effectively amplified in a sample of 100 individuals. These 17 loci, comprising four tri-nucleotide and 13 tetra-nucleotide repeats, exhibited a range of 4 to 26 alleles per locus (with an average of 14.1 alleles) across 100 samples from three O. stimpsoni populations. The mean observed and expected heterozygosities were calculated to be 0.885 and 0.836, respectively. These 17 newly identified polymorphic microsatellite loci hold valuable utility for investigating the genetic structure and diversity of this protected marine species. |
| format | Article |
| id | doaj-art-807789a79cd347238cfb32f082b9f16c |
| institution | Kabale University |
| issn | 1756-0500 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj-art-807789a79cd347238cfb32f082b9f16c2025-01-05T12:07:35ZengBMCBMC Research Notes1756-05002024-12-011711710.1186/s13104-024-07049-3Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae)Ha Yeun Song0Young Ji Choi1Dea-Sung Lee2Hyun Ki Choi3Kyung Min Choi4Kang-Yeol Yu5Namki Cho6Seungki Lee7Honam National Institute of Biological ResourcesHonam National Institute of Biological ResourcesNational Marine Biodiversity Institute of KoreaNational Institute of Biological ResourcesHonam National Institute of Biological ResourcesHonam National Institute of Biological ResourcesCollege of Pharmacy, Chonnam National UniversityNational Institute of Biological ResourcesAbstract Objective The ghost crab Ocypode stimpsoni (Decapoda) is designated as a protected marine species in Korea due to its declining population. In this study, we successfully identified 17 microsatellite markers for O. stimpsoni through next-generation sequencing. Results Out of the 63 loci examined, 26 were effectively amplified in a sample of 100 individuals. These 17 loci, comprising four tri-nucleotide and 13 tetra-nucleotide repeats, exhibited a range of 4 to 26 alleles per locus (with an average of 14.1 alleles) across 100 samples from three O. stimpsoni populations. The mean observed and expected heterozygosities were calculated to be 0.885 and 0.836, respectively. These 17 newly identified polymorphic microsatellite loci hold valuable utility for investigating the genetic structure and diversity of this protected marine species.https://doi.org/10.1186/s13104-024-07049-3Ocypode stimpsoniMicrosatellite lociGenetic variabilityConservation |
| spellingShingle | Ha Yeun Song Young Ji Choi Dea-Sung Lee Hyun Ki Choi Kyung Min Choi Kang-Yeol Yu Namki Cho Seungki Lee Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae) BMC Research Notes Ocypode stimpsoni Microsatellite loci Genetic variability Conservation |
| title | Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae) |
| title_full | Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae) |
| title_fullStr | Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae) |
| title_full_unstemmed | Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae) |
| title_short | Identification and characterization of 17 novel polynucleotide microsatellite markers in Ocypode stimpsoni (Decapoda: Ocypodidae) |
| title_sort | identification and characterization of 17 novel polynucleotide microsatellite markers in ocypode stimpsoni decapoda ocypodidae |
| topic | Ocypode stimpsoni Microsatellite loci Genetic variability Conservation |
| url | https://doi.org/10.1186/s13104-024-07049-3 |
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