Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4
ObjectiveThe aim of this study was to investigate the effect of curcumin nanocrystals (Cur-NCs) on ferroptosis in high-glucose (HG)-induced HK-2 cells and streptozotocin (STZ)-induced diabetic nephropathy model (DN) rats. The purpose is to determine whether Cur NCs can become a promising treatment o...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Pharmacology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fphar.2024.1508312/full |
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| author | Mengjiao Xue Mengjiao Xue Yiwei Tian Hua Zhang Hua Zhang Shijie Dai Yangsheng Wu Juan Jin Juan Jin Jian Chen |
| author_facet | Mengjiao Xue Mengjiao Xue Yiwei Tian Hua Zhang Hua Zhang Shijie Dai Yangsheng Wu Juan Jin Juan Jin Jian Chen |
| author_sort | Mengjiao Xue |
| collection | DOAJ |
| description | ObjectiveThe aim of this study was to investigate the effect of curcumin nanocrystals (Cur-NCs) on ferroptosis in high-glucose (HG)-induced HK-2 cells and streptozotocin (STZ)-induced diabetic nephropathy model (DN) rats. The purpose is to determine whether Cur NCs can become a promising treatment option for diabetes nephropathy by reducing ferroptosis.MethodsCur-NCs were prepared using microfluidic technology and studied using dynamic light scattering and transmission electron microscopy. HK-2 cells were treated with 30 mM HG to create a renal tubule damage cell model. Then, cell viability was evaluated in HK-2 cells treated with varying concentrations of Cur-NCs (0.23, 0.47, 0.94, 1.87, 3.75, 7.5, 15, and 30 μg/mL) using Cell Counting Kit-8 (CCK-8). Furthermore, in vivo experiments were carried out to investigate the roles of Cur-NCs in STZ-induced DN rats.ResultsThe results showed that HG treatment greatly enhanced the levels of LDH, MDA, Iron, lipid ROS, apoptosis, NCOA4, TFR-1, while decreasing the expression of GSH, GPX4, SLC7A11, and FTH-1. These effects induced by HG could be attenuated by Cur-NCs. Cur-NCs also reduced the HG-induced decrease in cell viability, as well as the increase in lipid ROS and cell apoptosis, however erastin could inhibit their effects. Furthermore, the in vivo results showed that Cur-NCs reduced ferroptosis and inhibited renal damage in DN rats.ConclusionThis study demonstrates that Cur-NCs can significantly attenuate ferroptosis in a STZ-induced renal damage model by recovering GPX4, implying that Cur-NCs may be a promising therapy option for DN. |
| format | Article |
| id | doaj-art-804b2010b05240918e59a47ea2d9cba9 |
| institution | Kabale University |
| issn | 1663-9812 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Pharmacology |
| spelling | doaj-art-804b2010b05240918e59a47ea2d9cba92025-01-06T06:59:00ZengFrontiers Media S.A.Frontiers in Pharmacology1663-98122025-01-011510.3389/fphar.2024.15083121508312Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4Mengjiao Xue0Mengjiao Xue1Yiwei Tian2Hua Zhang3Hua Zhang4Shijie Dai5Yangsheng Wu6Juan Jin7Juan Jin8Jian Chen9School of Clinical Medicine, Hangzhou Medical College, Hangzhou, ChinaDepartment of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, ChinaSchool of Pharmacy, Shanghai Jiaotong University, Shanghai, ChinaSchool of Clinical Medicine, Hangzhou Medical College, Hangzhou, ChinaDepartment of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, ChinaCollege of Life Science, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, ChinaCollege of Life Science, Academy of Chinese Medical Sciences, Zhejiang Chinese Medical University, Hangzhou, ChinaSchool of Clinical Medicine, Hangzhou Medical College, Hangzhou, ChinaDepartment of Nephrology, the First Affiliated Hospital of Zhejiang Chinese Medical University (Zhejiang Provincial Hospital of Chinese Medicine), Hangzhou, ChinaSchool of Pharmacy, Shanghai Jiaotong University, Shanghai, ChinaObjectiveThe aim of this study was to investigate the effect of curcumin nanocrystals (Cur-NCs) on ferroptosis in high-glucose (HG)-induced HK-2 cells and streptozotocin (STZ)-induced diabetic nephropathy model (DN) rats. The purpose is to determine whether Cur NCs can become a promising treatment option for diabetes nephropathy by reducing ferroptosis.MethodsCur-NCs were prepared using microfluidic technology and studied using dynamic light scattering and transmission electron microscopy. HK-2 cells were treated with 30 mM HG to create a renal tubule damage cell model. Then, cell viability was evaluated in HK-2 cells treated with varying concentrations of Cur-NCs (0.23, 0.47, 0.94, 1.87, 3.75, 7.5, 15, and 30 μg/mL) using Cell Counting Kit-8 (CCK-8). Furthermore, in vivo experiments were carried out to investigate the roles of Cur-NCs in STZ-induced DN rats.ResultsThe results showed that HG treatment greatly enhanced the levels of LDH, MDA, Iron, lipid ROS, apoptosis, NCOA4, TFR-1, while decreasing the expression of GSH, GPX4, SLC7A11, and FTH-1. These effects induced by HG could be attenuated by Cur-NCs. Cur-NCs also reduced the HG-induced decrease in cell viability, as well as the increase in lipid ROS and cell apoptosis, however erastin could inhibit their effects. Furthermore, the in vivo results showed that Cur-NCs reduced ferroptosis and inhibited renal damage in DN rats.ConclusionThis study demonstrates that Cur-NCs can significantly attenuate ferroptosis in a STZ-induced renal damage model by recovering GPX4, implying that Cur-NCs may be a promising therapy option for DN.https://www.frontiersin.org/articles/10.3389/fphar.2024.1508312/fullcurcuminnanocrystalsferroptosisdiabetic nephropathyGPX4 |
| spellingShingle | Mengjiao Xue Mengjiao Xue Yiwei Tian Hua Zhang Hua Zhang Shijie Dai Yangsheng Wu Juan Jin Juan Jin Jian Chen Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 Frontiers in Pharmacology curcumin nanocrystals ferroptosis diabetic nephropathy GPX4 |
| title | Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 |
| title_full | Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 |
| title_fullStr | Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 |
| title_full_unstemmed | Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 |
| title_short | Curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 |
| title_sort | curcumin nanocrystals ameliorate ferroptosis of diabetic nephropathy through glutathione peroxidase 4 |
| topic | curcumin nanocrystals ferroptosis diabetic nephropathy GPX4 |
| url | https://www.frontiersin.org/articles/10.3389/fphar.2024.1508312/full |
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