Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells
Abstract Purpose Antihyperglycemic drug artemisinin (Art) has recently gained attention as a potential anticancer treatment. In this study, the poly(lactic-co-glycolic acid) (PLGA) polymer was used to create Art-containing nanofibers (NFs) using the electrospinning technique. Methods The morphologic...
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BMC
2024-11-01
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| Series: | Cancer Nanotechnology |
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| Online Access: | https://doi.org/10.1186/s12645-024-00296-w |
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| author | Mohammad Eslami Vaghar Mehdi Dadashpour Elahe Derakhshan Vahid Vahedian Seyed Abbas Shahrtash Akram Firouzi Amandi Majid Eslami Maliheh Hasannia Zahra Mehrabi Leila Roshangar |
| author_facet | Mohammad Eslami Vaghar Mehdi Dadashpour Elahe Derakhshan Vahid Vahedian Seyed Abbas Shahrtash Akram Firouzi Amandi Majid Eslami Maliheh Hasannia Zahra Mehrabi Leila Roshangar |
| author_sort | Mohammad Eslami Vaghar |
| collection | DOAJ |
| description | Abstract Purpose Antihyperglycemic drug artemisinin (Art) has recently gained attention as a potential anticancer treatment. In this study, the poly(lactic-co-glycolic acid) (PLGA) polymer was used to create Art-containing nanofibers (NFs) using the electrospinning technique. Methods The morphological characteristics, rate of degradation, and drug release profile of the NFs were described. In addition, we examined both the cytotoxic effects and internalization of reactive oxygen species (ROS), as well as the expression levels of apoptotic genes following the treatment of SK-BR-3 breast cancer cells with Art and Art-loaded PLGA nanofibers. Results The bead-free, smooth surface, and randomly aligned electrospun NFs released the medication quickly at first and then steadily for more than 2 weeks. They also showed a rather steady rate of deterioration over the course of 24 days. After 48h, SK-BR-3 cells exposed to ART-loaded NFs shown a substantial cytotoxicity compared to free Art. Additionally, Art-loaded NFs effectively increased intracellular ROS levels, inducing death in cancer cells. Gene expression studies further demonstrated the ability of the produced Art-loaded NFs to significantly modify Bax and Bcl-2 levels as well as activate caspases-3 and P53 compared to free Art. Conclusion Overall, the results demonstrated the Art-loaded PLGA nanofibers anticancer effectiveness, indicating that it may be employed as an implantable drug delivery system to decrease breast cancer recurrence following surgical resection. |
| format | Article |
| id | doaj-art-7fc29c7296e14fa4a63ac5814bf6169d |
| institution | Kabale University |
| issn | 1868-6958 1868-6966 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | Cancer Nanotechnology |
| spelling | doaj-art-7fc29c7296e14fa4a63ac5814bf6169d2024-11-24T12:10:55ZengBMCCancer Nanotechnology1868-69581868-69662024-11-0115111610.1186/s12645-024-00296-wArtemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cellsMohammad Eslami Vaghar0Mehdi Dadashpour1Elahe Derakhshan2Vahid Vahedian3Seyed Abbas Shahrtash4Akram Firouzi Amandi5Majid Eslami6Maliheh Hasannia7Zahra Mehrabi8Leila Roshangar9Faculty of Medicine, Tehran Medical Sciences, Islamic Azad UniversityDepartment of Medical Biotechnology, Faculty of Medicine, Semnan University of Medical SciencesDepartment of Biotechnology, Faculty of Chemical Engineering, Tarbiat Modares UniversityDepartment of Hematology, Transfusion Medicine and Cellular Therapy/Cell Therapy Center (CTC-USP), Chemical Hospital and Cancer Institute (ICESP), Faculty of Medicine, University of Sao Paulo (FMUSP-HC)Department of Pharmaceutical Engineering, University of TehranDepartment of Immunology, Faculty of Medicine, Tabriz University of Medical SciencesDepartment of Bacteriology and Virology, Faculty of Medicine, Semnan University of Medical SciencesCancer Research Center, Semnan University of Medical SciencesDepartment of Clinical Biochemistry, Medical School, Kermanshah University of Medical SciencesStem Cell Research Center, Tabriz University of Medical SciencesAbstract Purpose Antihyperglycemic drug artemisinin (Art) has recently gained attention as a potential anticancer treatment. In this study, the poly(lactic-co-glycolic acid) (PLGA) polymer was used to create Art-containing nanofibers (NFs) using the electrospinning technique. Methods The morphological characteristics, rate of degradation, and drug release profile of the NFs were described. In addition, we examined both the cytotoxic effects and internalization of reactive oxygen species (ROS), as well as the expression levels of apoptotic genes following the treatment of SK-BR-3 breast cancer cells with Art and Art-loaded PLGA nanofibers. Results The bead-free, smooth surface, and randomly aligned electrospun NFs released the medication quickly at first and then steadily for more than 2 weeks. They also showed a rather steady rate of deterioration over the course of 24 days. After 48h, SK-BR-3 cells exposed to ART-loaded NFs shown a substantial cytotoxicity compared to free Art. Additionally, Art-loaded NFs effectively increased intracellular ROS levels, inducing death in cancer cells. Gene expression studies further demonstrated the ability of the produced Art-loaded NFs to significantly modify Bax and Bcl-2 levels as well as activate caspases-3 and P53 compared to free Art. Conclusion Overall, the results demonstrated the Art-loaded PLGA nanofibers anticancer effectiveness, indicating that it may be employed as an implantable drug delivery system to decrease breast cancer recurrence following surgical resection.https://doi.org/10.1186/s12645-024-00296-wArtemisininElectrospinningNanofiberPLGABreast cancer |
| spellingShingle | Mohammad Eslami Vaghar Mehdi Dadashpour Elahe Derakhshan Vahid Vahedian Seyed Abbas Shahrtash Akram Firouzi Amandi Majid Eslami Maliheh Hasannia Zahra Mehrabi Leila Roshangar Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells Cancer Nanotechnology Artemisinin Electrospinning Nanofiber PLGA Breast cancer |
| title | Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells |
| title_full | Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells |
| title_fullStr | Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells |
| title_full_unstemmed | Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells |
| title_short | Artemisinin-loaded mesoporous silica nanoparticles/electrospun poly(lactic-co-glycolic acid) composite nanofibers for enhanced anticancer efficiency in breast cancer cells |
| title_sort | artemisinin loaded mesoporous silica nanoparticles electrospun poly lactic co glycolic acid composite nanofibers for enhanced anticancer efficiency in breast cancer cells |
| topic | Artemisinin Electrospinning Nanofiber PLGA Breast cancer |
| url | https://doi.org/10.1186/s12645-024-00296-w |
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