The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> study
The aim of the study was to investigate the influence of the original porous silver–containing sorbent on the morphofunctional parameters of red blood cells during in vitro hemoperfusion. Material and methods. Donor blood was perfused through glass columns filled with a sorbent based on porous alumi...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | Russian |
| Published: |
Russian Academy of Sciences, Siberian Branch Publishing House
2022-10-01
|
| Series: | Сибирский научный медицинский журнал |
| Subjects: | |
| Online Access: | https://sibmed.elpub.ru/jour/article/view/881 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849318793546825728 |
|---|---|
| author | L. N. Rachkovskaya A. A. Smagin V. V. Nimaev A. Yu. Demura E. E. Rachkovsky E. S. Yastrebova V. P. Maltsev M. A. Korolev A. Yu. Letyagin |
| author_facet | L. N. Rachkovskaya A. A. Smagin V. V. Nimaev A. Yu. Demura E. E. Rachkovsky E. S. Yastrebova V. P. Maltsev M. A. Korolev A. Yu. Letyagin |
| author_sort | L. N. Rachkovskaya |
| collection | DOAJ |
| description | The aim of the study was to investigate the influence of the original porous silver–containing sorbent on the morphofunctional parameters of red blood cells during in vitro hemoperfusion. Material and methods. Donor blood was perfused through glass columns filled with a sorbent based on porous aluminum oxide, polydimethylsiloxane and silver nanoclusters and a sorbent without silver. The effect of a silver-containing sorbent on the change in morphofunctional parameters of red blood cells after perfusion through sorbents was determined by scanning flow cytometry. Results and their discussion. Due to the uniformity of the distribution of silver (0.1 %) over the sorbent granules, the parameters of the porous structure – the specific surface area and pore volume – practically do not change compared to the sorbent without silver. Morphological parameters of original donor blood and after hemoperfusion are within the norm. The functional parameters are also normal, although the introduction of silver in to the sorbent slightly increases the number of active band 3 (B3) proteins on erythrocyte membranes, both in comparison with the donor red cell mass as a control and in comparison with the sorbent without silver. There is also an increase in the ultimate extensibility of the erythrocyte membrane compared to the original blood (2.2 times) and the sorbent without silver (1.4 times). Conclusions. A sorbent modified with silver and a sorbent without silver does not have a damaging toxic effect on the morphofunctional parameters of blood under perfusion conditions. The mechanisms affecting the indicators of the ultimate extensibility of the erythrocyte membrane after blood perfusion through a silver-containing sorbent require further research. |
| format | Article |
| id | doaj-art-7f9e7d5233b64fe599c44efe119d3fa0 |
| institution | Kabale University |
| issn | 2410-2512 2410-2520 |
| language | Russian |
| publishDate | 2022-10-01 |
| publisher | Russian Academy of Sciences, Siberian Branch Publishing House |
| record_format | Article |
| series | Сибирский научный медицинский журнал |
| spelling | doaj-art-7f9e7d5233b64fe599c44efe119d3fa02025-08-20T03:50:44ZrusRussian Academy of Sciences, Siberian Branch Publishing HouseСибирский научный медицинский журнал2410-25122410-25202022-10-01425192410.18699/SSMJ20220503447The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> studyL. N. Rachkovskaya0A. A. Smagin1V. V. Nimaev2A. Yu. Demura3E. E. Rachkovsky4E. S. Yastrebova5V. P. Maltsev6M. A. Korolev7A. Yu. Letyagin8Recearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics of SB RASRecearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics of SB RASRecearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics of SB RASRecearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics of SB RASRecearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics of SB RASVoevodsky Institute of Chemical Kinetics and Combustion of SB RASVoevodsky Institute of Chemical Kinetics and Combustion of SB RASRecearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics of SB RASRecearch Institute of Clinical and Experimental Lymphology – Branch of the Institute of Cytology and Genetics of SB RASThe aim of the study was to investigate the influence of the original porous silver–containing sorbent on the morphofunctional parameters of red blood cells during in vitro hemoperfusion. Material and methods. Donor blood was perfused through glass columns filled with a sorbent based on porous aluminum oxide, polydimethylsiloxane and silver nanoclusters and a sorbent without silver. The effect of a silver-containing sorbent on the change in morphofunctional parameters of red blood cells after perfusion through sorbents was determined by scanning flow cytometry. Results and their discussion. Due to the uniformity of the distribution of silver (0.1 %) over the sorbent granules, the parameters of the porous structure – the specific surface area and pore volume – practically do not change compared to the sorbent without silver. Morphological parameters of original donor blood and after hemoperfusion are within the norm. The functional parameters are also normal, although the introduction of silver in to the sorbent slightly increases the number of active band 3 (B3) proteins on erythrocyte membranes, both in comparison with the donor red cell mass as a control and in comparison with the sorbent without silver. There is also an increase in the ultimate extensibility of the erythrocyte membrane compared to the original blood (2.2 times) and the sorbent without silver (1.4 times). Conclusions. A sorbent modified with silver and a sorbent without silver does not have a damaging toxic effect on the morphofunctional parameters of blood under perfusion conditions. The mechanisms affecting the indicators of the ultimate extensibility of the erythrocyte membrane after blood perfusion through a silver-containing sorbent require further research.https://sibmed.elpub.ru/jour/article/view/881sorbentsilveraluminum oxidepolydimethylsiloxaneerythrocytessafety |
| spellingShingle | L. N. Rachkovskaya A. A. Smagin V. V. Nimaev A. Yu. Demura E. E. Rachkovsky E. S. Yastrebova V. P. Maltsev M. A. Korolev A. Yu. Letyagin The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> study Сибирский научный медицинский журнал sorbent silver aluminum oxide polydimethylsiloxane erythrocytes safety |
| title | The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> study |
| title_full | The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> study |
| title_fullStr | The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> study |
| title_full_unstemmed | The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> study |
| title_short | The effect of silver-containing sorbent on red blood cells during hemosorption: an <i>in vitro</i> study |
| title_sort | effect of silver containing sorbent on red blood cells during hemosorption an i in vitro i study |
| topic | sorbent silver aluminum oxide polydimethylsiloxane erythrocytes safety |
| url | https://sibmed.elpub.ru/jour/article/view/881 |
| work_keys_str_mv | AT lnrachkovskaya theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT aasmagin theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT vvnimaev theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT ayudemura theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT eerachkovsky theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT esyastrebova theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT vpmaltsev theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT makorolev theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT ayuletyagin theeffectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT lnrachkovskaya effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT aasmagin effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT vvnimaev effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT ayudemura effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT eerachkovsky effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT esyastrebova effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT vpmaltsev effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT makorolev effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy AT ayuletyagin effectofsilvercontainingsorbentonredbloodcellsduringhemosorptionaniinvitroistudy |