Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoids
Dogs are increasingly recognized as valuable large animal models for understanding human intestinal diseases, as they naturally develop conditions similar to those in humans, such as Enterohemorrhagic E. coli, Clostridium difficile infection, inflammatory bowel disease, and ulcerative colitis. Given...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1483421/full |
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| author | Shino Yoshida Meg Nakazawa Minae Kawasaki Yoko M. Ambrosini |
| author_facet | Shino Yoshida Meg Nakazawa Minae Kawasaki Yoko M. Ambrosini |
| author_sort | Shino Yoshida |
| collection | DOAJ |
| description | Dogs are increasingly recognized as valuable large animal models for understanding human intestinal diseases, as they naturally develop conditions similar to those in humans, such as Enterohemorrhagic E. coli, Clostridium difficile infection, inflammatory bowel disease, and ulcerative colitis. Given the similarity in gut flora between dogs and humans, canine in vitro intestinal models are ideal for translational research. However, conventional extracellular matrix-embedded organoids present challenges in accessing the lumen, which is critical for gut function. This study aimed to investigate the feasibility of inducing polarity reversal and differentiation in canine apical-out colonic organoids (colonoids), evaluate their barrier integrity, and visualize host-pathogen interactions. Our results demonstrated successful polarity reversal and differentiation induction while maintaining barrier integrity. Polarity reversal allowed for enhanced observation of host-pathogen interactions, facilitating visual assessments and membrane integrity evaluations using both pathogenic and nonpathogenic E. coli. This process led to the downregulation of stem cell marker LGR5 and upregulation of intestinal epithelial cell marker ALPI, indicating differentiation. Further differentiation was observed with the use of a differentiation culture medium, resulting in significant upregulation of ALPI and goblet cell marker MUC2. The findings suggest that apical-out canine colonoids can serve as physiologic and valuable models for studying the pathogenic mechanisms and clinical significance of intestinal diseases in dogs. This model has the potential to advance both canine and human gastrointestinal research, enhancing our understanding of gastrointestinal physiology and pathology and aiding in the development of novel therapeutics. |
| format | Article |
| id | doaj-art-7f128acb31eb4dcb9435da4c7e81d51a |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-7f128acb31eb4dcb9435da4c7e81d51a2024-12-18T06:43:58ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692024-12-011110.3389/fvets.2024.14834211483421Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoidsShino YoshidaMeg NakazawaMinae KawasakiYoko M. AmbrosiniDogs are increasingly recognized as valuable large animal models for understanding human intestinal diseases, as they naturally develop conditions similar to those in humans, such as Enterohemorrhagic E. coli, Clostridium difficile infection, inflammatory bowel disease, and ulcerative colitis. Given the similarity in gut flora between dogs and humans, canine in vitro intestinal models are ideal for translational research. However, conventional extracellular matrix-embedded organoids present challenges in accessing the lumen, which is critical for gut function. This study aimed to investigate the feasibility of inducing polarity reversal and differentiation in canine apical-out colonic organoids (colonoids), evaluate their barrier integrity, and visualize host-pathogen interactions. Our results demonstrated successful polarity reversal and differentiation induction while maintaining barrier integrity. Polarity reversal allowed for enhanced observation of host-pathogen interactions, facilitating visual assessments and membrane integrity evaluations using both pathogenic and nonpathogenic E. coli. This process led to the downregulation of stem cell marker LGR5 and upregulation of intestinal epithelial cell marker ALPI, indicating differentiation. Further differentiation was observed with the use of a differentiation culture medium, resulting in significant upregulation of ALPI and goblet cell marker MUC2. The findings suggest that apical-out canine colonoids can serve as physiologic and valuable models for studying the pathogenic mechanisms and clinical significance of intestinal diseases in dogs. This model has the potential to advance both canine and human gastrointestinal research, enhancing our understanding of gastrointestinal physiology and pathology and aiding in the development of novel therapeutics.https://www.frontiersin.org/articles/10.3389/fvets.2024.1483421/fullorganoidcaninepolarity reversalapical-outintestinedifferentiation |
| spellingShingle | Shino Yoshida Meg Nakazawa Minae Kawasaki Yoko M. Ambrosini Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoids Frontiers in Veterinary Science organoid canine polarity reversal apical-out intestine differentiation |
| title | Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoids |
| title_full | Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoids |
| title_fullStr | Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoids |
| title_full_unstemmed | Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoids |
| title_short | Bacterial attachment and junctional transport function in induced apical-out polarized and differentiated canine intestinal organoids |
| title_sort | bacterial attachment and junctional transport function in induced apical out polarized and differentiated canine intestinal organoids |
| topic | organoid canine polarity reversal apical-out intestine differentiation |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1483421/full |
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