Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral <i>Galaxea fascicularis</i>: Revealing Key Proteins Related to Gamete Maturation and Fertilization
Sexually propagated scleractinian corals are in high demand for coral reef restoration. However, for threatened reef-building corals, many of the molecular mechanisms related to their reproduction remain largely unknown, which forms a major bottleneck in the large-scale cultivation of sexually repro...
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MDPI AG
2024-12-01
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| Series: | Journal of Marine Science and Engineering |
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| Online Access: | https://www.mdpi.com/2077-1312/12/12/2341 |
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| author | Yinyin Zhou Jingzhao Ke Lingyu Zheng Shaoyang Mo Xiangbo Liu He Zhao Wentao Zhu Xiubao Li |
| author_facet | Yinyin Zhou Jingzhao Ke Lingyu Zheng Shaoyang Mo Xiangbo Liu He Zhao Wentao Zhu Xiubao Li |
| author_sort | Yinyin Zhou |
| collection | DOAJ |
| description | Sexually propagated scleractinian corals are in high demand for coral reef restoration. However, for threatened reef-building corals, many of the molecular mechanisms related to their reproduction remain largely unknown, which forms a major bottleneck in the large-scale cultivation of sexually reproducing corals. In this study, we analyzed the proteomic signatures of red eggs and spermatozoa from the ecologically significant coral <i>Galaxea fascicularis</i>, using a data-independent acquisition mass spectrometry (DIA-MS) method. A total of 7741 and 7279 proteins from mature red eggs and spermatozoa were identified, respectively. Among these proteins, 596 proteins were spermatozoa-specific and 1056 were egg-specific. Additionally, a total of 4413 differentially abundant proteins (DAPs) were identified, among which 3121 proteins were up-regulated in red eggs and 1292 proteins were up-regulated in spermatozoa. Furthermore, anenrichment analyses showed that DAPs identified in red eggs were mainly involved in the progesterone-mediated oocyte maturation pathway and lectin pathway; and DAPs detected in spermatozoa were mainly involved in the insulin secretion pathway and metabolic pathways for the generation of energy. This result will contribute to the discovery of the intrinsic regulation pathway of gamete maturation and fertilization. Furthermore, at least 57 proteins associated with gamete maturation and reproduction were identified, including the red fluorescent protein (RFP), vitellogenin proteins (VG), the egg protein (EP), the testis-specific serine/threonine-protein kinase family (TSSKs), and the EF-hand Ca<sup>2+</sup>-binding protein family (EFHC1 and EFHC2). Particularly, the third yolk protein EUPHY was reported for the first time in <i>G. fascicularis</i>. In conclusion, this study unveiled groundbreaking molecular insights into coral sexual reproduction, paving the way for more effective conservation and sustainable development of coral reef ecosystems |
| format | Article |
| id | doaj-art-7f0871bf8b3346459cea6caf6b9b3119 |
| institution | Kabale University |
| issn | 2077-1312 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Marine Science and Engineering |
| spelling | doaj-art-7f0871bf8b3346459cea6caf6b9b31192024-12-27T14:33:39ZengMDPI AGJournal of Marine Science and Engineering2077-13122024-12-011212234110.3390/jmse12122341Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral <i>Galaxea fascicularis</i>: Revealing Key Proteins Related to Gamete Maturation and FertilizationYinyin Zhou0Jingzhao Ke1Lingyu Zheng2Shaoyang Mo3Xiangbo Liu4He Zhao5Wentao Zhu6Xiubao Li7School of Marine Biology and Fisheries, Hainan University, Haikou 570228, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou 570228, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou 570228, ChinaSchool of Marine Science and Engineering, Hainan University, Haikou 570228, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou 570228, ChinaCollege of Ecology and Environment, Hainan University, Haikou 570228, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou 570228, ChinaSchool of Marine Biology and Fisheries, Hainan University, Haikou 570228, ChinaSexually propagated scleractinian corals are in high demand for coral reef restoration. However, for threatened reef-building corals, many of the molecular mechanisms related to their reproduction remain largely unknown, which forms a major bottleneck in the large-scale cultivation of sexually reproducing corals. In this study, we analyzed the proteomic signatures of red eggs and spermatozoa from the ecologically significant coral <i>Galaxea fascicularis</i>, using a data-independent acquisition mass spectrometry (DIA-MS) method. A total of 7741 and 7279 proteins from mature red eggs and spermatozoa were identified, respectively. Among these proteins, 596 proteins were spermatozoa-specific and 1056 were egg-specific. Additionally, a total of 4413 differentially abundant proteins (DAPs) were identified, among which 3121 proteins were up-regulated in red eggs and 1292 proteins were up-regulated in spermatozoa. Furthermore, anenrichment analyses showed that DAPs identified in red eggs were mainly involved in the progesterone-mediated oocyte maturation pathway and lectin pathway; and DAPs detected in spermatozoa were mainly involved in the insulin secretion pathway and metabolic pathways for the generation of energy. This result will contribute to the discovery of the intrinsic regulation pathway of gamete maturation and fertilization. Furthermore, at least 57 proteins associated with gamete maturation and reproduction were identified, including the red fluorescent protein (RFP), vitellogenin proteins (VG), the egg protein (EP), the testis-specific serine/threonine-protein kinase family (TSSKs), and the EF-hand Ca<sup>2+</sup>-binding protein family (EFHC1 and EFHC2). Particularly, the third yolk protein EUPHY was reported for the first time in <i>G. fascicularis</i>. In conclusion, this study unveiled groundbreaking molecular insights into coral sexual reproduction, paving the way for more effective conservation and sustainable development of coral reef ecosystemshttps://www.mdpi.com/2077-1312/12/12/2341coral<i>Galaxea fascicularis</i>sexual reproductioncoral reef restorationDIA-based quantitative proteomics |
| spellingShingle | Yinyin Zhou Jingzhao Ke Lingyu Zheng Shaoyang Mo Xiangbo Liu He Zhao Wentao Zhu Xiubao Li Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral <i>Galaxea fascicularis</i>: Revealing Key Proteins Related to Gamete Maturation and Fertilization Journal of Marine Science and Engineering coral <i>Galaxea fascicularis</i> sexual reproduction coral reef restoration DIA-based quantitative proteomics |
| title | Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral <i>Galaxea fascicularis</i>: Revealing Key Proteins Related to Gamete Maturation and Fertilization |
| title_full | Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral <i>Galaxea fascicularis</i>: Revealing Key Proteins Related to Gamete Maturation and Fertilization |
| title_fullStr | Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral <i>Galaxea fascicularis</i>: Revealing Key Proteins Related to Gamete Maturation and Fertilization |
| title_full_unstemmed | Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral <i>Galaxea fascicularis</i>: Revealing Key Proteins Related to Gamete Maturation and Fertilization |
| title_short | Data-Independent Acquisition-Based Quantitative Proteomics Analysis of Fertile Red Eggs and Spermatozoa in Hermatypic Coral <i>Galaxea fascicularis</i>: Revealing Key Proteins Related to Gamete Maturation and Fertilization |
| title_sort | data independent acquisition based quantitative proteomics analysis of fertile red eggs and spermatozoa in hermatypic coral i galaxea fascicularis i revealing key proteins related to gamete maturation and fertilization |
| topic | coral <i>Galaxea fascicularis</i> sexual reproduction coral reef restoration DIA-based quantitative proteomics |
| url | https://www.mdpi.com/2077-1312/12/12/2341 |
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