Effects of Aerobic Exercise on Leukocyte-Mediated Liver Destruction in a Rat Model of Metabolic Syndrome
Objective: In this study, the levels of malondialdehyde (MDA), myeloperoxidase (MPO), and 3-nitrotyrosine (3-NT), which are known as oxidative/nitrosative stress markers, were investigated in the liver tissues of rats with metabolic syndrome model induced by a high fructose diet, and the possible pr...
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| Format: | Article |
| Language: | English |
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Galenos Publishing House
2024-10-01
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| Series: | Gazi Medical Journal |
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| Online Access: | https://gazimedj.com/articles/effects-of-aerobic-exercise-on-leukocyte-mediated-liver-destruction-in-a-rat-model-of-metabolic-syndrome/doi/gmj.2024.4178 |
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| author | Fatmanur Er Leyla Çimen Ceren Suveren Canan Yılmaz Nurten Türközkan |
| author_facet | Fatmanur Er Leyla Çimen Ceren Suveren Canan Yılmaz Nurten Türközkan |
| author_sort | Fatmanur Er |
| collection | DOAJ |
| description | Objective: In this study, the levels of malondialdehyde (MDA), myeloperoxidase (MPO), and 3-nitrotyrosine (3-NT), which are known as oxidative/nitrosative stress markers, were investigated in the liver tissues of rats with metabolic syndrome model induced by a high fructose diet, and the possible protective effects of aerobic exercise in fructose-fed rats were determined.
Methods: Rats were divided into four groups: control, fructose, exercise, and fructose plus exercise. Metabolic syndrome was induced in rats using 20% (w/v) fructose solution in tap water, and exercise was administered every day at the same hour for an experimental period of 8 weeks in total, 30 min a day, five days a week. After eight weeks, systolic blood pressure (SBP), serum lipid, glucose, insulin, MDA MPO, and 3-NT levels were quantified.
Results: The metabolic syndrome model was successfully demonstrated by fructose administration. Compared with the C group, F caused a significant increase in SBP, serum insulin, and triglyceride levels and liver MDA, MPO, and 3-NT levels. Exercise counteracted and healed the changes in SBP, serum insulin, triglyceride, and liver MDA, MPO, and 3-NT levels in fructose-fed rats (p<0.05).
Conclusion: These results indicate that high fructose consumption causes metabolic syndrome in rats, and aerobic exercise has beneficial effects on the components of metabolic syndrome. Exercise not only reduces the known risk factors of the disease, but also protects the liver while preventing oxidative and nitrosative damage caused by the MPO-H2O2 system in the liver, which increases with the effect of fructose and is necessary for the formation of non-alcoholic fatty liver disease. |
| format | Article |
| id | doaj-art-7ef765ecd42f4790bddc5492ecc6b9f8 |
| institution | Kabale University |
| issn | 2147-2092 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Galenos Publishing House |
| record_format | Article |
| series | Gazi Medical Journal |
| spelling | doaj-art-7ef765ecd42f4790bddc5492ecc6b9f82025-01-16T10:52:31ZengGalenos Publishing HouseGazi Medical Journal2147-20922024-10-0135440140610.12996/gmj.2024.4178Effects of Aerobic Exercise on Leukocyte-Mediated Liver Destruction in a Rat Model of Metabolic SyndromeFatmanur Er0https://orcid.org/0000-0002-9203-4974Leyla Çimen1https://orcid.org/0000-0003-0692-6421Ceren Suveren2https://orcid.org/0000-0002-2698-1500Canan Yılmaz3https://orcid.org/0000-0002-6799-6522Nurten Türközkan4https://orcid.org/0009-0009-9104-5661Department of Action and Training, Atatürk University Faculty of Sports Sciences, Erzurum, TürkiyeDepartment of Medical Biochemistry, Faculty of Medicine, Gaziantep Islam Science and Technology University, Gaziantep, TürkiyeDepartment of Training Sciences, Gazi University Faculty of Sports Sciences, Ankara, TürkiyeDepartment of Biochemistry, Faculty of Medicine, Gazi University, Ankara, TürkiyeRetiredObjective: In this study, the levels of malondialdehyde (MDA), myeloperoxidase (MPO), and 3-nitrotyrosine (3-NT), which are known as oxidative/nitrosative stress markers, were investigated in the liver tissues of rats with metabolic syndrome model induced by a high fructose diet, and the possible protective effects of aerobic exercise in fructose-fed rats were determined. Methods: Rats were divided into four groups: control, fructose, exercise, and fructose plus exercise. Metabolic syndrome was induced in rats using 20% (w/v) fructose solution in tap water, and exercise was administered every day at the same hour for an experimental period of 8 weeks in total, 30 min a day, five days a week. After eight weeks, systolic blood pressure (SBP), serum lipid, glucose, insulin, MDA MPO, and 3-NT levels were quantified. Results: The metabolic syndrome model was successfully demonstrated by fructose administration. Compared with the C group, F caused a significant increase in SBP, serum insulin, and triglyceride levels and liver MDA, MPO, and 3-NT levels. Exercise counteracted and healed the changes in SBP, serum insulin, triglyceride, and liver MDA, MPO, and 3-NT levels in fructose-fed rats (p<0.05). Conclusion: These results indicate that high fructose consumption causes metabolic syndrome in rats, and aerobic exercise has beneficial effects on the components of metabolic syndrome. Exercise not only reduces the known risk factors of the disease, but also protects the liver while preventing oxidative and nitrosative damage caused by the MPO-H2O2 system in the liver, which increases with the effect of fructose and is necessary for the formation of non-alcoholic fatty liver disease.https://gazimedj.com/articles/effects-of-aerobic-exercise-on-leukocyte-mediated-liver-destruction-in-a-rat-model-of-metabolic-syndrome/doi/gmj.2024.4178metabolic syndromenon-alcoholic fatty liver diseasemalondialdehydemyeloperoxidase3-nitrotyrosine |
| spellingShingle | Fatmanur Er Leyla Çimen Ceren Suveren Canan Yılmaz Nurten Türközkan Effects of Aerobic Exercise on Leukocyte-Mediated Liver Destruction in a Rat Model of Metabolic Syndrome Gazi Medical Journal metabolic syndrome non-alcoholic fatty liver disease malondialdehyde myeloperoxidase 3-nitrotyrosine |
| title | Effects of Aerobic Exercise on Leukocyte-Mediated Liver Destruction in a Rat Model of Metabolic Syndrome |
| title_full | Effects of Aerobic Exercise on Leukocyte-Mediated Liver Destruction in a Rat Model of Metabolic Syndrome |
| title_fullStr | Effects of Aerobic Exercise on Leukocyte-Mediated Liver Destruction in a Rat Model of Metabolic Syndrome |
| title_full_unstemmed | Effects of Aerobic Exercise on Leukocyte-Mediated Liver Destruction in a Rat Model of Metabolic Syndrome |
| title_short | Effects of Aerobic Exercise on Leukocyte-Mediated Liver Destruction in a Rat Model of Metabolic Syndrome |
| title_sort | effects of aerobic exercise on leukocyte mediated liver destruction in a rat model of metabolic syndrome |
| topic | metabolic syndrome non-alcoholic fatty liver disease malondialdehyde myeloperoxidase 3-nitrotyrosine |
| url | https://gazimedj.com/articles/effects-of-aerobic-exercise-on-leukocyte-mediated-liver-destruction-in-a-rat-model-of-metabolic-syndrome/doi/gmj.2024.4178 |
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