Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses
The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. In this work, we report a clarification of the phylogenetic relationship between <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis</i> via the screening of...
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2023-11-01
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| author | Sabah Ben Fredj Melki Ehi Sheena Omoregie Ahmed Mliki |
| author_facet | Sabah Ben Fredj Melki Ehi Sheena Omoregie Ahmed Mliki |
| author_sort | Sabah Ben Fredj Melki |
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| description | The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. In this work, we report a clarification of the phylogenetic relationship between <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis</i> via the screening of ITS-5.8RDNA, (niiA-niaD), ß-tubulin, and eEF-1 genes. The phylogenetic analysis of aspergilli ITS-5.8rDNA sequences divided <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis</i> into two coherent clusters and showed a close intergeneric relationship, which is in keeping with the existing morphological and taxonomic classification. Herein, <i>A. carbonarius</i> exhibits a complete similarity (100%) for the three target genes: intergenic region of (niiA-niaD), ß-tubulin, and eEF-1. The tree resulting from a bootstrap analysis indicates branch points when the significance is 98% minimum for each gene (ß-tubulin and eEF-1). The phylogenetic analysis of the aspergilli genome structure provided a quantitative evaluation of the forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis.</i> This is the first work describing the evolutionary relationship between both species. Nevertheless, this discovery might be viewed as typical during the process of new taxonomic evolution. |
| format | Article |
| id | doaj-art-7ee1a1eba863434c84d8e8f2a4458d26 |
| institution | Kabale University |
| issn | 2673-9976 |
| language | English |
| publishDate | 2023-11-01 |
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| spelling | doaj-art-7ee1a1eba863434c84d8e8f2a4458d262024-12-27T14:14:38ZengMDPI AGBiology and Life Sciences Forum2673-99762023-11-013113110.3390/ECM2023-16445Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence AnalysesSabah Ben Fredj Melki0Ehi Sheena Omoregie1Ahmed Mliki2Phytopathology and Detection Methodology Unit (PMDV) Mycomol Laboratory, INRAE Versailles Route de Saint Cyr, 78026 Versailles, FranceClinical Biochemistry & Toxicology Laboratory, University of Benin (UNIBEN), Nigeria 300213, BeninCenter of Biotechnology of Borj Cerdria-Tunisia, Laboratory of Molecular Plant Physiology, B.P. 901, Hammam-Lif 2050, TunisiaThe aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. In this work, we report a clarification of the phylogenetic relationship between <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis</i> via the screening of ITS-5.8RDNA, (niiA-niaD), ß-tubulin, and eEF-1 genes. The phylogenetic analysis of aspergilli ITS-5.8rDNA sequences divided <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis</i> into two coherent clusters and showed a close intergeneric relationship, which is in keeping with the existing morphological and taxonomic classification. Herein, <i>A. carbonarius</i> exhibits a complete similarity (100%) for the three target genes: intergenic region of (niiA-niaD), ß-tubulin, and eEF-1. The tree resulting from a bootstrap analysis indicates branch points when the significance is 98% minimum for each gene (ß-tubulin and eEF-1). The phylogenetic analysis of the aspergilli genome structure provided a quantitative evaluation of the forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis.</i> This is the first work describing the evolutionary relationship between both species. Nevertheless, this discovery might be viewed as typical during the process of new taxonomic evolution.https://www.mdpi.com/2673-9976/31/1/31<i>Aspergillus carbonarius</i><i>Aspergillus tubingensis</i>ß-tubulineEF-1ITS-5.8rDNAniiA-niaD |
| spellingShingle | Sabah Ben Fredj Melki Ehi Sheena Omoregie Ahmed Mliki Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses Biology and Life Sciences Forum <i>Aspergillus carbonarius</i> <i>Aspergillus tubingensis</i> ß-tubulin eEF-1 ITS-5.8rDNA niiA-niaD |
| title | Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses |
| title_full | Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses |
| title_fullStr | Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses |
| title_full_unstemmed | Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses |
| title_short | Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses |
| title_sort | unraveling phylogenetic relationships via em a carbonarius em and em a tubingensis em sequence analyses |
| topic | <i>Aspergillus carbonarius</i> <i>Aspergillus tubingensis</i> ß-tubulin eEF-1 ITS-5.8rDNA niiA-niaD |
| url | https://www.mdpi.com/2673-9976/31/1/31 |
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