Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses

Unraveling Phylogenetic Relationships via <em>A. carbonarius</em> and <em>A. tubingensis</em> Sequence Analyses

The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. In this work, we report a clarification of the phylogenetic relationship between <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis</i> via the screening of...

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Bibliographic Details
Main Authors: Sabah Ben Fredj Melki, Ehi Sheena Omoregie, Ahmed Mliki
Format: Article
Language:English
Published: MDPI AG 2023-11-01
Series:Biology and Life Sciences Forum
Subjects:
<i>Aspergillus carbonarius</i>
<i>Aspergillus tubingensis</i>
ß-tubulin
eEF-1
ITS-5.8rDNA
niiA-niaD
Online Access:https://www.mdpi.com/2673-9976/31/1/31
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Summary:The aspergilli comprise a diverse group of filamentous fungi spanning over 200 million years of evolution. In this work, we report a clarification of the phylogenetic relationship between <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis</i> via the screening of ITS-5.8RDNA, (niiA-niaD), ß-tubulin, and eEF-1 genes. The phylogenetic analysis of aspergilli ITS-5.8rDNA sequences divided <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis</i> into two coherent clusters and showed a close intergeneric relationship, which is in keeping with the existing morphological and taxonomic classification. Herein, <i>A. carbonarius</i> exhibits a complete similarity (100%) for the three target genes: intergenic region of (niiA-niaD), ß-tubulin, and eEF-1. The tree resulting from a bootstrap analysis indicates branch points when the significance is 98% minimum for each gene (ß-tubulin and eEF-1). The phylogenetic analysis of the aspergilli genome structure provided a quantitative evaluation of the forces driving long-term eukaryotic genome evolution. It also led to an experimentally validated model of mating-type locus evolution, suggesting the potential for sexual reproduction in <i>A</i>. <i>carbonarius</i> and <i>A. tubingensis.</i> This is the first work describing the evolutionary relationship between both species. Nevertheless, this discovery might be viewed as typical during the process of new taxonomic evolution.
ISSN:2673-9976

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