Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background
Over the past decade, the use of human stem cell-derived β cells (SC-β cells) to model pancreatic β cell development, function and disease has become increasingly common. Though protocols are rapidly improving, current directed differentiation strategies do not yield a pure population of insulin-pos...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-12-01
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| Series: | Stem Cell Research |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1873506124002575 |
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| author | Karla F. Leavens Catherine Osorio-Quintero Elisabeth Ashlyne Yeuteuh Francesca T. Perez-Profeta Anna Ada Dattoli Fabian L. Cardenas-Diaz Deborah L. French Paul Gadue |
| author_facet | Karla F. Leavens Catherine Osorio-Quintero Elisabeth Ashlyne Yeuteuh Francesca T. Perez-Profeta Anna Ada Dattoli Fabian L. Cardenas-Diaz Deborah L. French Paul Gadue |
| author_sort | Karla F. Leavens |
| collection | DOAJ |
| description | Over the past decade, the use of human stem cell-derived β cells (SC-β cells) to model pancreatic β cell development, function and disease has become increasingly common. Though protocols are rapidly improving, current directed differentiation strategies do not yield a pure population of insulin-positive SC-β cells in vitro. Therefore, it is experimentally advantageous to have reporter lines that allow for live sorting of insulin-positive populations. To aid in these studies, we have knocked mNeonGreen fluorescent protein into the endogenous insulin locus of the commonly used H1 (WA01) human embryonic stem cell line. |
| format | Article |
| id | doaj-art-7e9dd37f13724c6c96ca51f2e1be1d9f |
| institution | Kabale University |
| issn | 1873-5061 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Stem Cell Research |
| spelling | doaj-art-7e9dd37f13724c6c96ca51f2e1be1d9f2024-12-13T10:56:48ZengElsevierStem Cell Research1873-50612024-12-0181103559Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC backgroundKarla F. Leavens0Catherine Osorio-Quintero1Elisabeth Ashlyne Yeuteuh2Francesca T. Perez-Profeta3Anna Ada Dattoli4Fabian L. Cardenas-Diaz5Deborah L. French6Paul Gadue7Department of Pediatrics, Perelman School of Medicine, University of Pennsylvania and Division of Endocrinology and Diabetes, The Children’s Hospital of Philadelphia, Philadelphia, PA, USA; Corresponding author.Center for Cellular and Molecular Therapeutics, The Children’s Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia, Philadelphia, PA, USADepartment of Pediatrics, Perelman School of Medicine, University of Pennsylvania and Division of Endocrinology and Diabetes, The Children’s Hospital of Philadelphia, Philadelphia, PA, USADepartment of Pediatrics, Perelman School of Medicine, University of Pennsylvania and Division of Endocrinology and Diabetes, The Children’s Hospital of Philadelphia, Philadelphia, PA, USACenter for Cellular and Molecular Therapeutics, The Children’s Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia, Philadelphia, PA, USACenter for Cellular and Molecular Therapeutics, The Children’s Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia, Philadelphia, PA, USA; Department of Biology, School of Arts and Sciences, University of Pennsylvania, Philadelphia, PA, USACenter for Cellular and Molecular Therapeutics, The Children’s Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia, Philadelphia, PA, USACenter for Cellular and Molecular Therapeutics, The Children’s Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pathology and Laboratory Medicine, The Children’s Hospital of Philadelphia, Philadelphia, PA, USAOver the past decade, the use of human stem cell-derived β cells (SC-β cells) to model pancreatic β cell development, function and disease has become increasingly common. Though protocols are rapidly improving, current directed differentiation strategies do not yield a pure population of insulin-positive SC-β cells in vitro. Therefore, it is experimentally advantageous to have reporter lines that allow for live sorting of insulin-positive populations. To aid in these studies, we have knocked mNeonGreen fluorescent protein into the endogenous insulin locus of the commonly used H1 (WA01) human embryonic stem cell line.http://www.sciencedirect.com/science/article/pii/S1873506124002575 |
| spellingShingle | Karla F. Leavens Catherine Osorio-Quintero Elisabeth Ashlyne Yeuteuh Francesca T. Perez-Profeta Anna Ada Dattoli Fabian L. Cardenas-Diaz Deborah L. French Paul Gadue Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background Stem Cell Research |
| title | Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background |
| title_full | Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background |
| title_fullStr | Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background |
| title_full_unstemmed | Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background |
| title_short | Generation of a fluorescent mNeonGreen insulin reporter line in the H1 (WA01) hESC background |
| title_sort | generation of a fluorescent mneongreen insulin reporter line in the h1 wa01 hesc background |
| url | http://www.sciencedirect.com/science/article/pii/S1873506124002575 |
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