ANTIMICROBIAL ACTIVITY AND MECHANICAL PROPERTIES OF EDIBLE FILM ENRICHED WITH FINGERED CITRON WATER EXTRACT AND PROBIOTIC
Food consumption is vital, so edible and biodegradable materials are being developed against environmental factors. The aim of the study was to develop edible film containing fingered citron leaf extract and determine its biological activities and mechanical properties. In the study, the antimic...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Alma Mater Publishing House "Vasile Alecsandri" University of Bacau
2024-12-01
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| Series: | Scientific Study & Research: Chemistry & Chemical Engineering, Biotechnology, Food Industry |
| Subjects: | |
| Online Access: | https://pubs.ub.ro/?pg=revues&rev=cscc6&num=202404&vol=4&aid=5686 |
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| Summary: | Food consumption is vital, so edible and biodegradable
materials are being developed against environmental factors. The aim of the
study was to develop edible film containing fingered citron leaf extract and
determine its biological activities and mechanical properties. In the study,
the antimicrobial effect of fingered citron water (FCW) extract against
pathogens was determined by disc diffusion, micro and macro dilution tests.
Additionally, the antimicrobial activity of edible films containing the extract
and Limosilactobacillus fermentum MA-7 was determined by well diffusion
assay. The highest and lowest inhibition diameter of extract against
pathogens was detected for Escherichia coli O157:H7 (11.61 mm) and
Vibrio anguillarum A4 (9.09 mm). Minimal inhibition and bactericidal
concentration (MIC and MBC) values ranged between 5 - 40 µg·µL-1. Also,
it was determined that FCW extract prepared at 50 and 100 mg·mL-1
concentration had an inhibitory effect against E. coli O157:H7 at 0, 24 and
48th hours by viable cell count. Then, the antimicrobial activity of the
prepared films was evaluated and the highest and lowest inhibition zone
diameters against E. coli O157:H7 (12.85 mm) and Streptococcus
agalactiae Pas. Ins. (4.02 mm) were determined. It was determined that the
edible film prepared with FCW extract and probiotics has the potential to
protect against food spoilage. |
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| ISSN: | 1582-540X |