Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains
Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), threatens global food security and the livelihood of small-scale rice producers. Analyses of Xoo collections from Asia, Africa and the Americas demonstrated complete continental segregation, despite robust global rice...
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eLife Sciences Publications Ltd
2023-06-01
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| Online Access: | https://elifesciences.org/articles/84864 |
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| author | Van Schepler-Luu Coline Sciallano Melissa Stiebner Chonghui Ji Gabriel Boulard Amadou Diallo Florence Auguy Si Nian Char Yugander Arra Kyrylo Schenstnyi Marcel Buchholzer Eliza PI Loo Atugonza L Bilaro David Lihepanyama Mohammed Mkuya Rosemary Murori Ricardo Oliva Sebastien Cunnac Bing Yang Boris Szurek Wolf B Frommer |
| author_facet | Van Schepler-Luu Coline Sciallano Melissa Stiebner Chonghui Ji Gabriel Boulard Amadou Diallo Florence Auguy Si Nian Char Yugander Arra Kyrylo Schenstnyi Marcel Buchholzer Eliza PI Loo Atugonza L Bilaro David Lihepanyama Mohammed Mkuya Rosemary Murori Ricardo Oliva Sebastien Cunnac Bing Yang Boris Szurek Wolf B Frommer |
| author_sort | Van Schepler-Luu |
| collection | DOAJ |
| description | Bacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), threatens global food security and the livelihood of small-scale rice producers. Analyses of Xoo collections from Asia, Africa and the Americas demonstrated complete continental segregation, despite robust global rice trade. Here, we report unprecedented BB outbreaks in Tanzania. The causative strains, unlike endemic African Xoo, carry Asian-type TAL effectors targeting the sucrose transporter SWEET11a and iTALes suppressing Xa1. Phylogenomics clustered these strains with Xoo from Southern-China. African rice varieties do not carry effective resistance. To protect African rice production against this emerging threat, we developed a hybrid CRISPR-Cas9/Cpf1 system to edit all known TALe-binding elements in three SWEET promoters of the East African elite variety Komboka. The edited lines show broad-spectrum resistance against Asian and African strains of Xoo, including strains recently discovered in Tanzania. The strategy could help to protect global rice crops from BB pandemics. |
| format | Article |
| id | doaj-art-7de9fc58c42c46bcbcf943a0f4c8fcd5 |
| institution | Kabale University |
| issn | 2050-084X |
| language | English |
| publishDate | 2023-06-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj-art-7de9fc58c42c46bcbcf943a0f4c8fcd52025-01-07T17:37:33ZengeLife Sciences Publications LtdeLife2050-084X2023-06-011210.7554/eLife.84864Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strainsVan Schepler-Luu0https://orcid.org/0000-0002-0709-2783Coline Sciallano1https://orcid.org/0000-0001-8988-7733Melissa Stiebner2https://orcid.org/0000-0001-8626-0951Chonghui Ji3Gabriel Boulard4Amadou Diallo5Florence Auguy6Si Nian Char7https://orcid.org/0000-0002-5759-0764Yugander Arra8https://orcid.org/0000-0001-6778-4258Kyrylo Schenstnyi9https://orcid.org/0000-0003-1595-6382Marcel Buchholzer10https://orcid.org/0000-0001-7485-6918Eliza PI Loo11https://orcid.org/0000-0002-7622-1139Atugonza L Bilaro12David Lihepanyama13Mohammed Mkuya14Rosemary Murori15Ricardo Oliva16Sebastien Cunnac17Bing Yang18https://orcid.org/0000-0002-2293-3384Boris Szurek19https://orcid.org/0000-0002-1808-7082Wolf B Frommer20https://orcid.org/0000-0001-6465-0115Institute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany; International Rice Research Institute, Los Baños, PhilippinesPlant Health Institute of Montpellier (PHIM), Université Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, FranceInstitute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf, GermanyDivision of Plant Science and Technology, Bond Life Sciences Center, University of Missouri, Columbia, United StatesPlant Health Institute of Montpellier (PHIM), Université Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, FrancePlant Health Institute of Montpellier (PHIM), Université Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, FrancePlant Health Institute of Montpellier (PHIM), Université Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, FranceDivision of Plant Science and Technology, Bond Life Sciences Center, University of Missouri, Columbia, United StatesInstitute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf, GermanyInstitute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf, GermanyInstitute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf, GermanyInstitute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf, GermanyTanzania Agricultural Research Institute (TARI)-Uyole Centre, Mbeya, United Republic of TanzaniaTanzania Agricultural Research Institute (TARI)-Uyole Centre, Mbeya, United Republic of TanzaniaInternational Rice Research Institute, Eastern and Southern Africa Region, Nairobi, KenyaInternational Rice Research Institute (IRRI), Africa Regional Office, Nairobi, KenyaInternational Rice Research Institute, Los Baños, PhilippinesPlant Health Institute of Montpellier (PHIM), Université Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, FranceDivision of Plant Science and Technology, Bond Life Sciences Center, University of Missouri, Columbia, United States; Donald Danforth Plant Science Center, St. Louis, United StatesPlant Health Institute of Montpellier (PHIM), Université Montpellier, IRD, CIRAD, INRAE, Institut Agro, Montpellier, FranceInstitute for Molecular Physiology, Heinrich Heine University Düsseldorf, Düsseldorf, Germany; Institute for Transformative Biomolecules, ITbM, Nagoya University, Nagoya, JapanBacterial leaf blight (BB) of rice, caused by Xanthomonas oryzae pv. oryzae (Xoo), threatens global food security and the livelihood of small-scale rice producers. Analyses of Xoo collections from Asia, Africa and the Americas demonstrated complete continental segregation, despite robust global rice trade. Here, we report unprecedented BB outbreaks in Tanzania. The causative strains, unlike endemic African Xoo, carry Asian-type TAL effectors targeting the sucrose transporter SWEET11a and iTALes suppressing Xa1. Phylogenomics clustered these strains with Xoo from Southern-China. African rice varieties do not carry effective resistance. To protect African rice production against this emerging threat, we developed a hybrid CRISPR-Cas9/Cpf1 system to edit all known TALe-binding elements in three SWEET promoters of the East African elite variety Komboka. The edited lines show broad-spectrum resistance against Asian and African strains of Xoo, including strains recently discovered in Tanzania. The strategy could help to protect global rice crops from BB pandemics.https://elifesciences.org/articles/84864Oryza sativaXanthomonas oryzae pv. oryzaeAgrobacterium tumefacienspathogen |
| spellingShingle | Van Schepler-Luu Coline Sciallano Melissa Stiebner Chonghui Ji Gabriel Boulard Amadou Diallo Florence Auguy Si Nian Char Yugander Arra Kyrylo Schenstnyi Marcel Buchholzer Eliza PI Loo Atugonza L Bilaro David Lihepanyama Mohammed Mkuya Rosemary Murori Ricardo Oliva Sebastien Cunnac Bing Yang Boris Szurek Wolf B Frommer Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains eLife Oryza sativa Xanthomonas oryzae pv. oryzae Agrobacterium tumefaciens pathogen |
| title | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_full | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_fullStr | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_full_unstemmed | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_short | Genome editing of an African elite rice variety confers resistance against endemic and emerging Xanthomonas oryzae pv. oryzae strains |
| title_sort | genome editing of an african elite rice variety confers resistance against endemic and emerging xanthomonas oryzae pv oryzae strains |
| topic | Oryza sativa Xanthomonas oryzae pv. oryzae Agrobacterium tumefaciens pathogen |
| url | https://elifesciences.org/articles/84864 |
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