Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng
Abstract Background The extract from Metasequoia glyptostroboides Hu et Cheng, a rare and endangered species native to China, exhibits numerous biological and pharmacological activities. The species is recalcitrant to rooting during micropropagation, a challenge that has yet to be resolved. In this...
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2024-11-01
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| Online Access: | https://doi.org/10.1186/s12864-024-10989-6 |
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| author | Yuping Xiong Xiaohong Chen Junyu Liu Yuan Li Zhan Bian Xinhua Zhang Songjun Zeng Jaime A. Teixeira da Silva Guohua Ma |
| author_facet | Yuping Xiong Xiaohong Chen Junyu Liu Yuan Li Zhan Bian Xinhua Zhang Songjun Zeng Jaime A. Teixeira da Silva Guohua Ma |
| author_sort | Yuping Xiong |
| collection | DOAJ |
| description | Abstract Background The extract from Metasequoia glyptostroboides Hu et Cheng, a rare and endangered species native to China, exhibits numerous biological and pharmacological activities. The species is recalcitrant to rooting during micropropagation, a challenge that has yet to be resolved. In this study, transcriptomic and hormonal analyses were conducted to appreciate the molecular mechanism of adventitious root (AR) formation in optimized rooting conditions. Results The use of 2/5-strength Woody Plant Medium (WPM) significantly promoted AR formation of M. glyptostroboides shoots while the content of endogenous auxin, cytokinins and gibberellins (GAs) varied at different stages of AR formation. Transcriptomic analysis showed the significant up- or down-regulation of differentially expressed genes (DEGs) associated with plant hormone signal transduction and the phenylpropanoid biosynthesis pathway in response to 2/5-strength WPM. DEGs related to the biosynthesis of indole-3-acetic acid, cytokinins and GAs were identified. Transcript factors involved in 13 families were also revealed. A weighted gene co-expression network analysis indicated a strong correlation between hormones and genes involved in plant hormone signal transduction and the phenylpropanoid biosynthetic pathway. Conclusions These results indicate that the AR-promoting potential of 2/5-strength WPM in M. glyptostroboides was due to complex interactions between hormones and the expression of genes related to plant hormone signal transduction and the phenylpropanoid biosynthetic pathway. |
| format | Article |
| id | doaj-art-7dc97ae6a174435a937f52f1dae4b2b5 |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-7dc97ae6a174435a937f52f1dae4b2b52024-11-24T12:11:59ZengBMCBMC Genomics1471-21642024-11-0125112110.1186/s12864-024-10989-6Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et ChengYuping Xiong0Xiaohong Chen1Junyu Liu2Yuan Li3Zhan Bian4Xinhua Zhang5Songjun Zeng6Jaime A. Teixeira da Silva7Guohua Ma8Guangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of SciencesGuangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of SciencesIndependent ResearcherGuangdong Provincial Key Laboratory of Applied Botany, South China, Botanical Garden , Chinese Academy of SciencesAbstract Background The extract from Metasequoia glyptostroboides Hu et Cheng, a rare and endangered species native to China, exhibits numerous biological and pharmacological activities. The species is recalcitrant to rooting during micropropagation, a challenge that has yet to be resolved. In this study, transcriptomic and hormonal analyses were conducted to appreciate the molecular mechanism of adventitious root (AR) formation in optimized rooting conditions. Results The use of 2/5-strength Woody Plant Medium (WPM) significantly promoted AR formation of M. glyptostroboides shoots while the content of endogenous auxin, cytokinins and gibberellins (GAs) varied at different stages of AR formation. Transcriptomic analysis showed the significant up- or down-regulation of differentially expressed genes (DEGs) associated with plant hormone signal transduction and the phenylpropanoid biosynthesis pathway in response to 2/5-strength WPM. DEGs related to the biosynthesis of indole-3-acetic acid, cytokinins and GAs were identified. Transcript factors involved in 13 families were also revealed. A weighted gene co-expression network analysis indicated a strong correlation between hormones and genes involved in plant hormone signal transduction and the phenylpropanoid biosynthetic pathway. Conclusions These results indicate that the AR-promoting potential of 2/5-strength WPM in M. glyptostroboides was due to complex interactions between hormones and the expression of genes related to plant hormone signal transduction and the phenylpropanoid biosynthetic pathway.https://doi.org/10.1186/s12864-024-10989-6Metasequoia glyptostroboides Hu et ChengTranscriptomeEndogenous hormonesAdventitious root |
| spellingShingle | Yuping Xiong Xiaohong Chen Junyu Liu Yuan Li Zhan Bian Xinhua Zhang Songjun Zeng Jaime A. Teixeira da Silva Guohua Ma Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng BMC Genomics Metasequoia glyptostroboides Hu et Cheng Transcriptome Endogenous hormones Adventitious root |
| title | Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng |
| title_full | Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng |
| title_fullStr | Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng |
| title_full_unstemmed | Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng |
| title_short | Comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in Metasequoia glyptostroboides Hu et Cheng |
| title_sort | comparative transcriptomic and hormonal analyses reveal potential regulation networks of adventitious root formation in metasequoia glyptostroboides hu et cheng |
| topic | Metasequoia glyptostroboides Hu et Cheng Transcriptome Endogenous hormones Adventitious root |
| url | https://doi.org/10.1186/s12864-024-10989-6 |
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