Oocyte recovery and comparison of maturation rates of different quality oocytes in cattle
<b>Aim: </b>The aim of the presented study; oocyte number and quality obtained from bovine ovaries procured from abattoir and evaluation of the relationship between different quality oocyte maturation rate for in vitro embryo production.<p> <b>Materials and Methods:</b>...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2021-03-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1341 |
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| Summary: | <b>Aim: </b>The aim of the presented study; oocyte number and quality obtained
from bovine ovaries procured from abattoir and evaluation of
the relationship between different quality oocyte maturation rate for
in vitro embryo production.<p>
<b>Materials and Methods:</b> For this purpose, 370 ovaries were transported
with 0.9% isotonic sodium chloride containing 100,000 IU
penicilin-streptomycin and brought to the laboratory within 2-4 hours.
40 of the ovaries were not used in the study due to their cyst and
adhesion nature. 1894 oocytes were obtained from the remaining
330 ovaries by aspiration method. The quality of the obtained oocytes
was evaluated as A, B, C and D quality according to the cytoplasm
density and the distribution of the cumulus cells around the oocyte.
1384 of them, A and B quality, were left to maturation with a commercial
maturation medium (BO-IVM,Bioscience, UK)at 38.8 °C with
atmospheric O<sub>2</sub> at s5.5% CO<sub>2</sub> for 24 hours.
Results: With the aspiration method, including 800 A, 584 B, 450 C
and 60 D quality a total of 1894 oocytes were obtained. In addition,
it was determined that the number of 2.42 A, 1.76 B, 1.36 C and 0.18
D per ovary was reduced. Only A and B quality oocytes were used
for maturation. The maturation rate was determined as 90.3% in A
quality oocytes and 83.3% in B quality oocytes (p?0.01). The total
maturation rate of the study was determined as 87.4%.<p>
<b>Conclusion: </b>As a result, it was determined that maturation rates
change according to oocyte quality and maturation rates reduce with
the decrease in quality. Therefore, it was concluded that it is necessary
to evaluate the oocyte quality before maturation. |
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| ISSN: | 1309-6958 2146-1953 |