One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso
Data on antimicrobial resistance in Burkina Faso’s agricultural sector is still limited. This study assessed the occurrence of extended-spectrum β-lactamase-producing <i>Escherichia coli</i> (ESBL-<i>Ec</i>) and <i>Klebsiella</i> spp. (ESBL-<i>K</i>) i...
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MDPI AG
2024-10-01
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| author | Fatimata Bintou Josiane Diarra Isidore Juste Ouindgueta Bonkoungou Zakaria Garba Namwin Siourimè Somda Djifahamaï Soma Marguerite Edith Malatala Nikiema Evariste Bako Souleymane Sore Natéwindé Sawadogo Nicolas Barro Kaisa Haukka |
| author_facet | Fatimata Bintou Josiane Diarra Isidore Juste Ouindgueta Bonkoungou Zakaria Garba Namwin Siourimè Somda Djifahamaï Soma Marguerite Edith Malatala Nikiema Evariste Bako Souleymane Sore Natéwindé Sawadogo Nicolas Barro Kaisa Haukka |
| author_sort | Fatimata Bintou Josiane Diarra |
| collection | DOAJ |
| description | Data on antimicrobial resistance in Burkina Faso’s agricultural sector is still limited. This study assessed the occurrence of extended-spectrum β-lactamase-producing <i>Escherichia coli</i> (ESBL-<i>Ec</i>) and <i>Klebsiella</i> spp. (ESBL-<i>K</i>) in lettuce, environment, and gardeners’ stools in market gardens in Ouagadougou, Burkina Faso. A total of 356 samples were collected from three vegetable gardens (76 lettuce, 76 soil, 62 manure, 63 irrigation water, and 79 human stools). The ESBL-selective medium was used for initial selection of ESBL-producing bacteria, and the isolates were further identified using biochemical tests. An antibiotic susceptibility test was performed using the disk diffusion method. The overall prevalence of ESBL-<i>Ec</i> and/or ESBL-<i>K</i> in the samples was 232/356 (65.2%). Of the lettuce samples, the prevalence of ESBL-<i>Ec</i> was 19/76 (25.0%) and ESBL-<i>K</i> 33/76 (43.4%). In the market gardens environment, the prevalence of ESBL-<i>Ec</i> was 32/201 (15.9%) and ESBL-<i>K</i> 124/201 (61.7%). In the gardeners’ stools, the prevalence of ESBL-<i>Ec</i> was 42/79 (53.2%) and ESBL-<i>K</i> 24/79 (30.4%). Two ESBL-<i>K</i> isolates were found to produce NDM carbapenemase. Due to the high prevalence of ESBL-producing bacteria, which may also be carbapenemase producers, it is necessary to monitor pathogens in agricultural products with a “One Health” approach to limit and prevent infections in the population. |
| format | Article |
| id | doaj-art-7d90c45539c749bba29f0b676b53c3ad |
| institution | Kabale University |
| issn | 2076-2607 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Microorganisms |
| spelling | doaj-art-7d90c45539c749bba29f0b676b53c3ad2024-11-26T18:14:22ZengMDPI AGMicroorganisms2076-26072024-10-011211217010.3390/microorganisms12112170One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina FasoFatimata Bintou Josiane Diarra0Isidore Juste Ouindgueta Bonkoungou1Zakaria Garba2Namwin Siourimè Somda3Djifahamaï Soma4Marguerite Edith Malatala Nikiema5Evariste Bako6Souleymane Sore7Natéwindé Sawadogo8Nicolas Barro9Kaisa Haukka10Department of Biochemistry and Microbiology, Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoDepartment of Biochemistry and Microbiology, Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoClinical Research Unit of Nanoro, Institut de Recherche en Sciences de la Santé, Ouagadougou 11 BP 218, Burkina FasoDépartement Technologie Alimentaire (DTA)/IRSAT/CNRST, Ouagadougou 03 BP 7047, Burkina FasoDepartment of Biochemistry and Microbiology, Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoLaboratoire de Virologie et Biotechnologies Végétales, Institut de L’Environnement et de Recherches Agricoles (INERA), CNRST, Ouagadougou 04 BP 8645, Burkina FasoDepartment of Biochemistry and Microbiology, Centre Universitaire de Tenkodogo, Ouagadougou 12 BP 417, Burkina FasoDirection des Laboratoires de Biologie Médicale, Ministère de la Santé, Ouagadougou 03 BP 7022, Burkina FasoDepartment of Sociology, Université Thomas SANKARA, Ouagadougou 12 BP 417, Burkina FasoDepartment of Biochemistry and Microbiology, Université Joseph KI-ZERBO, Ouagadougou 03 BP 7021, Burkina FasoDepartment of Microbiology, University of Helsinki, 00014 Helsinki, FinlandData on antimicrobial resistance in Burkina Faso’s agricultural sector is still limited. This study assessed the occurrence of extended-spectrum β-lactamase-producing <i>Escherichia coli</i> (ESBL-<i>Ec</i>) and <i>Klebsiella</i> spp. (ESBL-<i>K</i>) in lettuce, environment, and gardeners’ stools in market gardens in Ouagadougou, Burkina Faso. A total of 356 samples were collected from three vegetable gardens (76 lettuce, 76 soil, 62 manure, 63 irrigation water, and 79 human stools). The ESBL-selective medium was used for initial selection of ESBL-producing bacteria, and the isolates were further identified using biochemical tests. An antibiotic susceptibility test was performed using the disk diffusion method. The overall prevalence of ESBL-<i>Ec</i> and/or ESBL-<i>K</i> in the samples was 232/356 (65.2%). Of the lettuce samples, the prevalence of ESBL-<i>Ec</i> was 19/76 (25.0%) and ESBL-<i>K</i> 33/76 (43.4%). In the market gardens environment, the prevalence of ESBL-<i>Ec</i> was 32/201 (15.9%) and ESBL-<i>K</i> 124/201 (61.7%). In the gardeners’ stools, the prevalence of ESBL-<i>Ec</i> was 42/79 (53.2%) and ESBL-<i>K</i> 24/79 (30.4%). Two ESBL-<i>K</i> isolates were found to produce NDM carbapenemase. Due to the high prevalence of ESBL-producing bacteria, which may also be carbapenemase producers, it is necessary to monitor pathogens in agricultural products with a “One Health” approach to limit and prevent infections in the population.https://www.mdpi.com/2076-2607/12/11/2170<i>Escherichia coli</i><i>Klebsiella</i> spp.ESBLmarket gardensBurkina Faso |
| spellingShingle | Fatimata Bintou Josiane Diarra Isidore Juste Ouindgueta Bonkoungou Zakaria Garba Namwin Siourimè Somda Djifahamaï Soma Marguerite Edith Malatala Nikiema Evariste Bako Souleymane Sore Natéwindé Sawadogo Nicolas Barro Kaisa Haukka One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso Microorganisms <i>Escherichia coli</i> <i>Klebsiella</i> spp. ESBL market gardens Burkina Faso |
| title | One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso |
| title_full | One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso |
| title_fullStr | One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso |
| title_full_unstemmed | One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso |
| title_short | One Health Approach to Study the Occurrence and Antimicrobial Resistance of Extended-Spectrum β-Lactamase- and Carbapenemase-Producing <i>Escherichia coli</i> and <i>Klebsiella</i> spp. in Urban Agriculture in Burkina Faso |
| title_sort | one health approach to study the occurrence and antimicrobial resistance of extended spectrum β lactamase and carbapenemase producing i escherichia coli i and i klebsiella i spp in urban agriculture in burkina faso |
| topic | <i>Escherichia coli</i> <i>Klebsiella</i> spp. ESBL market gardens Burkina Faso |
| url | https://www.mdpi.com/2076-2607/12/11/2170 |
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