Application of a Sensitive Capture Sequencing Approach to Reservoir Surveillance Detects Novel Viruses in Zambian Wild Rodents
We utilized a pan-viral capture sequencing assay, VirCapSeq-VERT, to assess viral diversity in rodents from the Eastern Province of Zambia as a model for pre-pandemic viral reservoir surveillance. We report rodent adeno-, parvo-, paramyxo-, and picornaviruses that represent novel species or isolates...
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2024-11-01
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| Online Access: | https://www.mdpi.com/1999-4915/16/11/1754 |
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| author | Lavel C. Moonga Jones Chipinga John P. Collins Vishal Kapoor Ngonda Saasa King S. Nalubamba Bernard M. Hang’ombe Boniface Namangala Tapiwa Lundu Xiang-Jun Lu Samuel Yingst J. Kenneth Wickiser Thomas Briese |
| author_facet | Lavel C. Moonga Jones Chipinga John P. Collins Vishal Kapoor Ngonda Saasa King S. Nalubamba Bernard M. Hang’ombe Boniface Namangala Tapiwa Lundu Xiang-Jun Lu Samuel Yingst J. Kenneth Wickiser Thomas Briese |
| author_sort | Lavel C. Moonga |
| collection | DOAJ |
| description | We utilized a pan-viral capture sequencing assay, VirCapSeq-VERT, to assess viral diversity in rodents from the Eastern Province of Zambia as a model for pre-pandemic viral reservoir surveillance. We report rodent adeno-, parvo-, paramyxo-, and picornaviruses that represent novel species or isolates, including murine adenovirus 4, two additional species in the genus <i>Chaphamaparvovirus</i>, two paramyxoviruses distantly related to unclassified viruses in the genus <i>Jeilongvirus</i>, and the first <i>Aichivirus A</i> sequence identified from rodents in Africa. Our results emphasize the importance of rodents as a reservoir for potential zoonotic viruses. |
| format | Article |
| id | doaj-art-7d78215185cd4f929d11c787af6a19bb |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-7d78215185cd4f929d11c787af6a19bb2024-11-26T18:25:33ZengMDPI AGViruses1999-49152024-11-011611175410.3390/v16111754Application of a Sensitive Capture Sequencing Approach to Reservoir Surveillance Detects Novel Viruses in Zambian Wild RodentsLavel C. Moonga0Jones Chipinga1John P. Collins2Vishal Kapoor3Ngonda Saasa4King S. Nalubamba5Bernard M. Hang’ombe6Boniface Namangala7Tapiwa Lundu8Xiang-Jun Lu9Samuel Yingst10J. Kenneth Wickiser11Thomas Briese12Department of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, ZambiaVaughan Regional Medical Center, Selma, AL 36701, USAGlobal Alliance for Preventing Pandemics at the Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USAGlobal Alliance for Preventing Pandemics at the Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USADepartment of Disease Control, School of Veterinary Medicine, University of Zambia, Lusaka 10101, ZambiaDepartment of Clinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, ZambiaDepartment of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, ZambiaDepartment of Paraclinical Studies, School of Veterinary Medicine, University of Zambia, Lusaka 10101, ZambiaDepartment of Biomedical Sciences, School of Veterinary Medicine, University of Zambia, Lusaka 10101, ZambiaGlobal Alliance for Preventing Pandemics at the Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USAGlobal Alliance for Preventing Pandemics at the Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USAGlobal Alliance for Preventing Pandemics at the Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USAGlobal Alliance for Preventing Pandemics at the Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USAWe utilized a pan-viral capture sequencing assay, VirCapSeq-VERT, to assess viral diversity in rodents from the Eastern Province of Zambia as a model for pre-pandemic viral reservoir surveillance. We report rodent adeno-, parvo-, paramyxo-, and picornaviruses that represent novel species or isolates, including murine adenovirus 4, two additional species in the genus <i>Chaphamaparvovirus</i>, two paramyxoviruses distantly related to unclassified viruses in the genus <i>Jeilongvirus</i>, and the first <i>Aichivirus A</i> sequence identified from rodents in Africa. Our results emphasize the importance of rodents as a reservoir for potential zoonotic viruses.https://www.mdpi.com/1999-4915/16/11/1754pre-pandemic surveillanceOne Healthpathogen discoveryadenoviruschaphamaparvovirusparamyxovirus |
| spellingShingle | Lavel C. Moonga Jones Chipinga John P. Collins Vishal Kapoor Ngonda Saasa King S. Nalubamba Bernard M. Hang’ombe Boniface Namangala Tapiwa Lundu Xiang-Jun Lu Samuel Yingst J. Kenneth Wickiser Thomas Briese Application of a Sensitive Capture Sequencing Approach to Reservoir Surveillance Detects Novel Viruses in Zambian Wild Rodents Viruses pre-pandemic surveillance One Health pathogen discovery adenovirus chaphamaparvovirus paramyxovirus |
| title | Application of a Sensitive Capture Sequencing Approach to Reservoir Surveillance Detects Novel Viruses in Zambian Wild Rodents |
| title_full | Application of a Sensitive Capture Sequencing Approach to Reservoir Surveillance Detects Novel Viruses in Zambian Wild Rodents |
| title_fullStr | Application of a Sensitive Capture Sequencing Approach to Reservoir Surveillance Detects Novel Viruses in Zambian Wild Rodents |
| title_full_unstemmed | Application of a Sensitive Capture Sequencing Approach to Reservoir Surveillance Detects Novel Viruses in Zambian Wild Rodents |
| title_short | Application of a Sensitive Capture Sequencing Approach to Reservoir Surveillance Detects Novel Viruses in Zambian Wild Rodents |
| title_sort | application of a sensitive capture sequencing approach to reservoir surveillance detects novel viruses in zambian wild rodents |
| topic | pre-pandemic surveillance One Health pathogen discovery adenovirus chaphamaparvovirus paramyxovirus |
| url | https://www.mdpi.com/1999-4915/16/11/1754 |
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