In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats
We evaluated the in vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats. First, in vitro antidiabetic activity was analysed through the inhibition of α-amylase and α-glucosidase. Then, the acute toxicity...
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Elsevier
2025-01-01
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| Series: | Journal of Functional Foods |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1756464624005784 |
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| author | Fabrícia de Souza Ferreira Hassler Clementino Cavalcante Larissa Maria Gomes Dutra Adriano Francisco Alves Stephson Andriola Almeida Gonçalves Tatiana Colombo Pimentel Carlos Eduardo Barão Marcos dos Santos Lima Alana Natalícia Vasconcelos de Araújo Januse Millia Dantas de Araújo Kamila Sabino Batista Jailane de Souza Aquino Angela Maria Tribuzy de Magalhães Cordeiro |
| author_facet | Fabrícia de Souza Ferreira Hassler Clementino Cavalcante Larissa Maria Gomes Dutra Adriano Francisco Alves Stephson Andriola Almeida Gonçalves Tatiana Colombo Pimentel Carlos Eduardo Barão Marcos dos Santos Lima Alana Natalícia Vasconcelos de Araújo Januse Millia Dantas de Araújo Kamila Sabino Batista Jailane de Souza Aquino Angela Maria Tribuzy de Magalhães Cordeiro |
| author_sort | Fabrícia de Souza Ferreira |
| collection | DOAJ |
| description | We evaluated the in vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats. First, in vitro antidiabetic activity was analysed through the inhibition of α-amylase and α-glucosidase. Then, the acute toxicity of mucilage was evaluated in Wistar rats for 14 days. After, an experiment to evaluate the effects of mucilage on obesity was conducted with 30 male Wistar rats randomised into three groups: healthy (HG); obese (OG); and obese treated with facheiro mucilage (FOG) by gavage (200 mg/kg). The HG received commercial diet and the OG and FOG received a cafeteria diet for 12 weeks. Facheiro mucilage showed antidiabetic activity in vitro by reducing the α-amylase (64.15 ± 3.86 %) and α-glucosidase activity (86.43 ± 1.09 %), without signs of acute toxicity in vivo. Mucilage treatment reduced insulinemia (OG = 29.70 ± 4.52 µIU/mL vs. FOG = 14.04 ± 5.08 µIU/mL, p = 0.01, effect size: 3.25), homeostasis assessment model (OG = 0.99 ± 0.12, FOG = 0.76 ± 0.18, p = 0.03, effect size: 1.50), uric acid (OG = 6.65 ± 1.63 mg/dL vs. FOG = 4.07 ± 0.65 mg/dL, p = 0.0001, effect size: 2.07), creatinine (OG = 0.90 ± 0.08 mg/dL vs. FOG = 1.05 ± 0.15 mg/dL, p = 0.0001, effect size: 1.24), alanine transaminase (OG = 29.70 ± 4.52 IU/L vs. FOG = 14.05 ± 5.08 IU/L, p = 0.0001, effect size: 3.25), aspartate transaminase (OG = 58.55 ± 7.10 IU/L vs. FOG = 22.35 ± 3.61 IU/L, p = 0.0001, effect size: 6.42) and the hepatic steatosis in the FOG compared to the OG. Mucilage intake increased the total concentrations of organic acids and sugars in renal and hepatic tissues of the FOG when compared to the OG. However, overall, it did not alter the murinometric parameters. Our results suggest that facheiro mucilage had antidiabetic activity in vitro, which corroborated the improvement of insulinemia and insulin resistance in FOG rats; in addition, it showed renoprotective and hepatoprotective effects, highlighting a reversal in the severity of hepatic steatosis in obese rats. |
| format | Article |
| id | doaj-art-7d34a323e37c47cc8193a59ca14cd2f5 |
| institution | Kabale University |
| issn | 1756-4646 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Functional Foods |
| spelling | doaj-art-7d34a323e37c47cc8193a59ca14cd2f52025-01-12T05:24:41ZengElsevierJournal of Functional Foods1756-46462025-01-01124106575In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese ratsFabrícia de Souza Ferreira0Hassler Clementino Cavalcante1Larissa Maria Gomes Dutra2Adriano Francisco Alves3Stephson Andriola Almeida Gonçalves4Tatiana Colombo Pimentel5Carlos Eduardo Barão6Marcos dos Santos Lima7Alana Natalícia Vasconcelos de Araújo8Januse Millia Dantas de Araújo9Kamila Sabino Batista10Jailane de Souza Aquino11Angela Maria Tribuzy de Magalhães Cordeiro12Experimental Nutrition Laboratory—LANEX, Department of Nutrition, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Post Graduate Program in Food Science and Technology, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, BrazilExperimental Nutrition Laboratory—LANEX, Department of Nutrition, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Post Graduate Program in Nutrition Sciences, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, BrazilExperimental Nutrition Laboratory—LANEX, Department of Nutrition, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Post Graduate Program in Food Science and Technology, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, BrazilDepartment of Physiology and Pathology, Federal University of Paraíba, 58051-900 João Pessoa, Paraíba, BrazilCitilab Clinical Analysis Laboratory, 58187-000 Picuí, Paraíba, BrazilFederal Institute of Paraná, Campus Paranavaí, 87703-536 Paranavaí, Paraná, BrazilFederal Institute of Paraná, Campus Paranavaí, 87703-536 Paranavaí, Paraná, BrazilDepartment of Food Technology, Federal Institute of Sertão Pernambucano (IF- Sertão PE), 56316-686 Petrolina, Pernambuco, BrazilExperimental Nutrition Laboratory—LANEX, Department of Nutrition, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Post Graduate Program in Nutrition Sciences, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, BrazilExperimental Nutrition Laboratory—LANEX, Department of Nutrition, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Post Graduate Program in Nutrition Sciences, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, BrazilNational Institute of Semi-Arid Regions – INSA, 58434-700 Campina Grande, Paraíba, BrazilExperimental Nutrition Laboratory—LANEX, Department of Nutrition, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Post Graduate Program in Food Science and Technology, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Post Graduate Program in Nutrition Sciences, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Corresponding author at: Experimental Nutrition Laboratory, Department of Nutrition, Federal University of Paraíba (UFPB), João Pessoa, Paraíba, Brazil.Post Graduate Program in Food Science and Technology, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, Brazil; Department of Food Science and Technology, Federal University of Paraíba (UFPB), Campus I, 58051-900 João Pessoa, Paraíba, BrazilWe evaluated the in vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats. First, in vitro antidiabetic activity was analysed through the inhibition of α-amylase and α-glucosidase. Then, the acute toxicity of mucilage was evaluated in Wistar rats for 14 days. After, an experiment to evaluate the effects of mucilage on obesity was conducted with 30 male Wistar rats randomised into three groups: healthy (HG); obese (OG); and obese treated with facheiro mucilage (FOG) by gavage (200 mg/kg). The HG received commercial diet and the OG and FOG received a cafeteria diet for 12 weeks. Facheiro mucilage showed antidiabetic activity in vitro by reducing the α-amylase (64.15 ± 3.86 %) and α-glucosidase activity (86.43 ± 1.09 %), without signs of acute toxicity in vivo. Mucilage treatment reduced insulinemia (OG = 29.70 ± 4.52 µIU/mL vs. FOG = 14.04 ± 5.08 µIU/mL, p = 0.01, effect size: 3.25), homeostasis assessment model (OG = 0.99 ± 0.12, FOG = 0.76 ± 0.18, p = 0.03, effect size: 1.50), uric acid (OG = 6.65 ± 1.63 mg/dL vs. FOG = 4.07 ± 0.65 mg/dL, p = 0.0001, effect size: 2.07), creatinine (OG = 0.90 ± 0.08 mg/dL vs. FOG = 1.05 ± 0.15 mg/dL, p = 0.0001, effect size: 1.24), alanine transaminase (OG = 29.70 ± 4.52 IU/L vs. FOG = 14.05 ± 5.08 IU/L, p = 0.0001, effect size: 3.25), aspartate transaminase (OG = 58.55 ± 7.10 IU/L vs. FOG = 22.35 ± 3.61 IU/L, p = 0.0001, effect size: 6.42) and the hepatic steatosis in the FOG compared to the OG. Mucilage intake increased the total concentrations of organic acids and sugars in renal and hepatic tissues of the FOG when compared to the OG. However, overall, it did not alter the murinometric parameters. Our results suggest that facheiro mucilage had antidiabetic activity in vitro, which corroborated the improvement of insulinemia and insulin resistance in FOG rats; in addition, it showed renoprotective and hepatoprotective effects, highlighting a reversal in the severity of hepatic steatosis in obese rats.http://www.sciencedirect.com/science/article/pii/S1756464624005784CactusHepatic steatosisInsulinObesityRenal functionToxicity |
| spellingShingle | Fabrícia de Souza Ferreira Hassler Clementino Cavalcante Larissa Maria Gomes Dutra Adriano Francisco Alves Stephson Andriola Almeida Gonçalves Tatiana Colombo Pimentel Carlos Eduardo Barão Marcos dos Santos Lima Alana Natalícia Vasconcelos de Araújo Januse Millia Dantas de Araújo Kamila Sabino Batista Jailane de Souza Aquino Angela Maria Tribuzy de Magalhães Cordeiro In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats Journal of Functional Foods Cactus Hepatic steatosis Insulin Obesity Renal function Toxicity |
| title | In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats |
| title_full | In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats |
| title_fullStr | In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats |
| title_full_unstemmed | In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats |
| title_short | In vitro antidiabetic activity of facheiro (Pilosocereus pachycladus) mucilage and its effects on glycaemic, renal and hepatic parameters in obese rats |
| title_sort | in vitro antidiabetic activity of facheiro pilosocereus pachycladus mucilage and its effects on glycaemic renal and hepatic parameters in obese rats |
| topic | Cactus Hepatic steatosis Insulin Obesity Renal function Toxicity |
| url | http://www.sciencedirect.com/science/article/pii/S1756464624005784 |
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