Ameliorative Effects of Raisin Polyphenol Extract on Oxidative Stress and Aging In Vitro and In Vivo via Regulation of Sirt1–Nrf2 Signaling Pathway
Raisins are an important source of polyphenolic compounds in plant foods, and polyphenols are associated with antioxidant and anti-aging activity. In this work, 628 polyphenols in raisin extracts were characterized using UPLC-MS/MS, mainly including tricetin 3′-glucuronide, diisobutyl phthalate, but...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-12-01
|
| Series: | Foods |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2304-8158/14/1/71 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1841549218954608640 |
|---|---|
| author | Wenjing Gao Caiyun Zhao Xin Shang Bin Li Jintian Guo Jingteng Wang Bin Wu Yinghua Fu |
| author_facet | Wenjing Gao Caiyun Zhao Xin Shang Bin Li Jintian Guo Jingteng Wang Bin Wu Yinghua Fu |
| author_sort | Wenjing Gao |
| collection | DOAJ |
| description | Raisins are an important source of polyphenolic compounds in plant foods, and polyphenols are associated with antioxidant and anti-aging activity. In this work, 628 polyphenols in raisin extracts were characterized using UPLC-MS/MS, mainly including tricetin 3′-glucuronide, diisobutyl phthalate, butyl isobutyl phthalate, isoquercitrin and 6-hydroxykaempferol-7-O-glucoside. The oxidative stress in H<sub>2</sub>O<sub>2</sub>-induced HepG2 cells and D-gal-induced aging mice was alleviated by raisin polyphenols (RPs) via increases in the cellular levels of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH), along with decreases in malonaldehyde (MDA), reactive oxygen species (ROS) and advanced glycosylation end-products (AGEs) levels. In addition, it was observed that RPs enhanced Sirt1 and Sirt3 expression, initiating the Keap1-Nrf2 signaling pathway, by upregulating the levels of nuclear Nrf2, facilitating the expressions of the antioxidant proteins NQO1 and HO-1, and downregulating Keap1 and cytoplasmic Nrf2 protein levels in H<sub>2</sub>O<sub>2</sub>-induced HepG2 cells and D-gal-induced aging mice. In summary, RP exerted antioxidant and anti-aging effects via regulating the Sirt1–Nrf2 signaling pathway in vitro and in vivo. |
| format | Article |
| id | doaj-art-7ce3b87b6a2747558978fd1e6d3e3f08 |
| institution | Kabale University |
| issn | 2304-8158 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj-art-7ce3b87b6a2747558978fd1e6d3e3f082025-01-10T13:17:42ZengMDPI AGFoods2304-81582024-12-011417110.3390/foods14010071Ameliorative Effects of Raisin Polyphenol Extract on Oxidative Stress and Aging In Vitro and In Vivo via Regulation of Sirt1–Nrf2 Signaling PathwayWenjing Gao0Caiyun Zhao1Xin Shang2Bin Li3Jintian Guo4Jingteng Wang5Bin Wu6Yinghua Fu7College of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaInstitute of Agro-Products Storage and Processing, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, ChinaCollege of Life Science and Technology, Xinjiang University, Urumqi 830017, ChinaRaisins are an important source of polyphenolic compounds in plant foods, and polyphenols are associated with antioxidant and anti-aging activity. In this work, 628 polyphenols in raisin extracts were characterized using UPLC-MS/MS, mainly including tricetin 3′-glucuronide, diisobutyl phthalate, butyl isobutyl phthalate, isoquercitrin and 6-hydroxykaempferol-7-O-glucoside. The oxidative stress in H<sub>2</sub>O<sub>2</sub>-induced HepG2 cells and D-gal-induced aging mice was alleviated by raisin polyphenols (RPs) via increases in the cellular levels of superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH), along with decreases in malonaldehyde (MDA), reactive oxygen species (ROS) and advanced glycosylation end-products (AGEs) levels. In addition, it was observed that RPs enhanced Sirt1 and Sirt3 expression, initiating the Keap1-Nrf2 signaling pathway, by upregulating the levels of nuclear Nrf2, facilitating the expressions of the antioxidant proteins NQO1 and HO-1, and downregulating Keap1 and cytoplasmic Nrf2 protein levels in H<sub>2</sub>O<sub>2</sub>-induced HepG2 cells and D-gal-induced aging mice. In summary, RP exerted antioxidant and anti-aging effects via regulating the Sirt1–Nrf2 signaling pathway in vitro and in vivo.https://www.mdpi.com/2304-8158/14/1/71raisin polyphenolsoxidative stressoxidatively damaged HepG2 cellsaging miceSirt1–Nrf2 signaling pathway |
| spellingShingle | Wenjing Gao Caiyun Zhao Xin Shang Bin Li Jintian Guo Jingteng Wang Bin Wu Yinghua Fu Ameliorative Effects of Raisin Polyphenol Extract on Oxidative Stress and Aging In Vitro and In Vivo via Regulation of Sirt1–Nrf2 Signaling Pathway Foods raisin polyphenols oxidative stress oxidatively damaged HepG2 cells aging mice Sirt1–Nrf2 signaling pathway |
| title | Ameliorative Effects of Raisin Polyphenol Extract on Oxidative Stress and Aging In Vitro and In Vivo via Regulation of Sirt1–Nrf2 Signaling Pathway |
| title_full | Ameliorative Effects of Raisin Polyphenol Extract on Oxidative Stress and Aging In Vitro and In Vivo via Regulation of Sirt1–Nrf2 Signaling Pathway |
| title_fullStr | Ameliorative Effects of Raisin Polyphenol Extract on Oxidative Stress and Aging In Vitro and In Vivo via Regulation of Sirt1–Nrf2 Signaling Pathway |
| title_full_unstemmed | Ameliorative Effects of Raisin Polyphenol Extract on Oxidative Stress and Aging In Vitro and In Vivo via Regulation of Sirt1–Nrf2 Signaling Pathway |
| title_short | Ameliorative Effects of Raisin Polyphenol Extract on Oxidative Stress and Aging In Vitro and In Vivo via Regulation of Sirt1–Nrf2 Signaling Pathway |
| title_sort | ameliorative effects of raisin polyphenol extract on oxidative stress and aging in vitro and in vivo via regulation of sirt1 nrf2 signaling pathway |
| topic | raisin polyphenols oxidative stress oxidatively damaged HepG2 cells aging mice Sirt1–Nrf2 signaling pathway |
| url | https://www.mdpi.com/2304-8158/14/1/71 |
| work_keys_str_mv | AT wenjinggao ameliorativeeffectsofraisinpolyphenolextractonoxidativestressandaginginvitroandinvivoviaregulationofsirt1nrf2signalingpathway AT caiyunzhao ameliorativeeffectsofraisinpolyphenolextractonoxidativestressandaginginvitroandinvivoviaregulationofsirt1nrf2signalingpathway AT xinshang ameliorativeeffectsofraisinpolyphenolextractonoxidativestressandaginginvitroandinvivoviaregulationofsirt1nrf2signalingpathway AT binli ameliorativeeffectsofraisinpolyphenolextractonoxidativestressandaginginvitroandinvivoviaregulationofsirt1nrf2signalingpathway AT jintianguo ameliorativeeffectsofraisinpolyphenolextractonoxidativestressandaginginvitroandinvivoviaregulationofsirt1nrf2signalingpathway AT jingtengwang ameliorativeeffectsofraisinpolyphenolextractonoxidativestressandaginginvitroandinvivoviaregulationofsirt1nrf2signalingpathway AT binwu ameliorativeeffectsofraisinpolyphenolextractonoxidativestressandaginginvitroandinvivoviaregulationofsirt1nrf2signalingpathway AT yinghuafu ameliorativeeffectsofraisinpolyphenolextractonoxidativestressandaginginvitroandinvivoviaregulationofsirt1nrf2signalingpathway |