Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regions
Polysaccharides from L. fructus (LFPs) serve as important active biomacromolecules for the wide spectrum of bioactivities exhibited by Lycii fructus. However, the influence of ecological environments on the biosynthesis and structural characteristics of LFPs remains largely unexplored. The present r...
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Elsevier
2025-06-01
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| Series: | Food Chemistry: Molecular Sciences |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S266656622400039X |
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| author | Limei Tong Yinxiu Jiang Xinrun Zhang Xia Zhang Yihao Zheng Qiheng Zhao Sheng Yu Wenhua Zhang Gang Ren Zhanping Chen Yuling Zhao Sheng Guo Hui Yan Shulan Su Yang Pan Jin-ao Duan Fang Zhang |
| author_facet | Limei Tong Yinxiu Jiang Xinrun Zhang Xia Zhang Yihao Zheng Qiheng Zhao Sheng Yu Wenhua Zhang Gang Ren Zhanping Chen Yuling Zhao Sheng Guo Hui Yan Shulan Su Yang Pan Jin-ao Duan Fang Zhang |
| author_sort | Limei Tong |
| collection | DOAJ |
| description | Polysaccharides from L. fructus (LFPs) serve as important active biomacromolecules for the wide spectrum of bioactivities exhibited by Lycii fructus. However, the influence of ecological environments on the biosynthesis and structural characteristics of LFPs remains largely unexplored. The present research conducted a comprehensive strategy combining physicochemical structural elucidation, stoichiometric analysis, transcriptomic profiling, and proteomic analysis in L. fructus samples collected from typical cultivation regions including Ningxia, Xinjiang, and Qinghai. The results revealed distinct structural variations in LFPs from Ningxia compared to those from other regions in terms of Glc, Ara, GalA composition percentages as well as overall content. The omics data identified 5531 and 8084 differentially expressed genes (DEGs), as well as 3728 and 4732 differentially expressed proteins (DEPs) in Xinjiang and Qinghai compared to Ningxia, respectively. The integration of transcriptomic and proteomic analyses revealed enrichment of DEGs and DEPs involved in the biosynthesis pathway of LFPs, including UDPs, AXS, and UGDH. Temperature and precipitation were the significant ecological factors affecting LFPs biosynthesis. These comprehensive analyses provide a novel perspective for explaining the important material basis and environmental response mechanism underlying the quality formation of L. fructus. |
| format | Article |
| id | doaj-art-7cc6c7dbd18540ce8dde8ced01f076a5 |
| institution | Kabale University |
| issn | 2666-5662 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: Molecular Sciences |
| spelling | doaj-art-7cc6c7dbd18540ce8dde8ced01f076a52024-12-11T05:57:40ZengElsevierFood Chemistry: Molecular Sciences2666-56622025-06-0110100232Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regionsLimei Tong0Yinxiu Jiang1Xinrun Zhang2Xia Zhang3Yihao Zheng4Qiheng Zhao5Sheng Yu6Wenhua Zhang7Gang Ren8Zhanping Chen9Yuling Zhao10Sheng Guo11Hui Yan12Shulan Su13Yang Pan14Jin-ao Duan15Fang Zhang16Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaSchool of Pharmacy, Key Laboratory of Minority Medicine Modernization, Ministry of Education, Ningxia Medical University, Yinchuan 750021, PR China; Corresponding authors.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; School of Global Public Health, New York University, New York, NY 10003, United StatesJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; School of Chinese Materia Medica, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaBairuiyuan Gouqi Co., Ltd, Yinchuan 750200, ChinaHaixi Agriculture and Animal Husbandry Technology Extension Service Center, Delingha 817000, ChinaHaixi Agriculture and Animal Husbandry Technology Extension Service Center, Delingha 817000, ChinaJinghe Gouqi Industry Development Center of Bortala Mongolian Autonomous Prefecture, Bortala 833399, ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR ChinaJiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; Corresponding authors.Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing 210023, PR China; Corresponding authors.Polysaccharides from L. fructus (LFPs) serve as important active biomacromolecules for the wide spectrum of bioactivities exhibited by Lycii fructus. However, the influence of ecological environments on the biosynthesis and structural characteristics of LFPs remains largely unexplored. The present research conducted a comprehensive strategy combining physicochemical structural elucidation, stoichiometric analysis, transcriptomic profiling, and proteomic analysis in L. fructus samples collected from typical cultivation regions including Ningxia, Xinjiang, and Qinghai. The results revealed distinct structural variations in LFPs from Ningxia compared to those from other regions in terms of Glc, Ara, GalA composition percentages as well as overall content. The omics data identified 5531 and 8084 differentially expressed genes (DEGs), as well as 3728 and 4732 differentially expressed proteins (DEPs) in Xinjiang and Qinghai compared to Ningxia, respectively. The integration of transcriptomic and proteomic analyses revealed enrichment of DEGs and DEPs involved in the biosynthesis pathway of LFPs, including UDPs, AXS, and UGDH. Temperature and precipitation were the significant ecological factors affecting LFPs biosynthesis. These comprehensive analyses provide a novel perspective for explaining the important material basis and environmental response mechanism underlying the quality formation of L. fructus.http://www.sciencedirect.com/science/article/pii/S266656622400039XLycii fructusPolysaccharidesBiosynthesisTranscriptomic analysisProteomic analysis |
| spellingShingle | Limei Tong Yinxiu Jiang Xinrun Zhang Xia Zhang Yihao Zheng Qiheng Zhao Sheng Yu Wenhua Zhang Gang Ren Zhanping Chen Yuling Zhao Sheng Guo Hui Yan Shulan Su Yang Pan Jin-ao Duan Fang Zhang Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regions Food Chemistry: Molecular Sciences Lycii fructus Polysaccharides Biosynthesis Transcriptomic analysis Proteomic analysis |
| title | Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regions |
| title_full | Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regions |
| title_fullStr | Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regions |
| title_full_unstemmed | Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regions |
| title_short | Integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of Lycii fructus polysaccharides from different cultivation regions |
| title_sort | integrated transcriptomic and proteomic analyses provide insights into the biosynthesis of lycii fructus polysaccharides from different cultivation regions |
| topic | Lycii fructus Polysaccharides Biosynthesis Transcriptomic analysis Proteomic analysis |
| url | http://www.sciencedirect.com/science/article/pii/S266656622400039X |
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