Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma Cells
Background: Ziziphus spina-christi (Rhamnaceae) is employed in Middle Eastern countries for the treatment of different diseases. Earlier reports revealed that it is cytotoxic to oral cancer cells. Objective: The current study determined the antioxidant activity and the anticancer potential of methan...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
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| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_631_24 |
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| _version_ | 1841544140170461184 |
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| author | Samir Abdulkarim Alharbi |
| author_facet | Samir Abdulkarim Alharbi |
| author_sort | Samir Abdulkarim Alharbi |
| collection | DOAJ |
| description | Background:
Ziziphus spina-christi (Rhamnaceae) is employed in Middle Eastern countries for the treatment of different diseases. Earlier reports revealed that it is cytotoxic to oral cancer cells.
Objective:
The current study determined the antioxidant activity and the anticancer potential of methanolic extract of Z. spina-christi against gastric carcinoma.
Materials and Methods:
LC-MS analysis was used to determine the different phytoconstituents predicted to be present in the extract. The effect of the Z. spina-christi leaf extract against human gastric carcinoma (AGS) cell lines was evaluated using MTT assay and cellular apoptosis techniques. A ferric-reducing antioxidant power assay was used to assess the antioxidant effect.
Results and Conclusion:
The LC-MS analysis revealed 24 phytoconstituents and Z. spina-christi extract was cytotoxic on AGS cells with an IC50 value of 125.1 μg/ml. Incubation of AGS cells with the extract decreased the percentage of live cells with a significant increase in late apoptotic phase and necrotic phase in the cellular apoptosis assay. The Z. spina-christi extract showed a potent antioxidant effect. The results show that Z. spina-christi extract possesses cytotoxic and antioxidant actions. |
| format | Article |
| id | doaj-art-7c7fdb7f4e804d82926d62663d97d489 |
| institution | Kabale University |
| issn | 0976-4879 0975-7406 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Pharmacy and Bioallied Sciences |
| spelling | doaj-art-7c7fdb7f4e804d82926d62663d97d4892025-01-12T14:21:38ZengWolters Kluwer Medknow PublicationsJournal of Pharmacy and Bioallied Sciences0976-48790975-74062024-12-0116Suppl 4S3354S335710.4103/jpbs.jpbs_631_24Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma CellsSamir Abdulkarim AlharbiBackground: Ziziphus spina-christi (Rhamnaceae) is employed in Middle Eastern countries for the treatment of different diseases. Earlier reports revealed that it is cytotoxic to oral cancer cells. Objective: The current study determined the antioxidant activity and the anticancer potential of methanolic extract of Z. spina-christi against gastric carcinoma. Materials and Methods: LC-MS analysis was used to determine the different phytoconstituents predicted to be present in the extract. The effect of the Z. spina-christi leaf extract against human gastric carcinoma (AGS) cell lines was evaluated using MTT assay and cellular apoptosis techniques. A ferric-reducing antioxidant power assay was used to assess the antioxidant effect. Results and Conclusion: The LC-MS analysis revealed 24 phytoconstituents and Z. spina-christi extract was cytotoxic on AGS cells with an IC50 value of 125.1 μg/ml. Incubation of AGS cells with the extract decreased the percentage of live cells with a significant increase in late apoptotic phase and necrotic phase in the cellular apoptosis assay. The Z. spina-christi extract showed a potent antioxidant effect. The results show that Z. spina-christi extract possesses cytotoxic and antioxidant actions.https://journals.lww.com/10.4103/jpbs.jpbs_631_24cellular apoptosisdna fragmentationgastric cancerlc-msmtt assay |
| spellingShingle | Samir Abdulkarim Alharbi Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma Cells Journal of Pharmacy and Bioallied Sciences cellular apoptosis dna fragmentation gastric cancer lc-ms mtt assay |
| title | Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma Cells |
| title_full | Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma Cells |
| title_fullStr | Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma Cells |
| title_full_unstemmed | Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma Cells |
| title_short | Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma Cells |
| title_sort | anticancer and antioxidant activities of methanolic extract of ziziphus spina christi leaves against human gastric carcinoma cells |
| topic | cellular apoptosis dna fragmentation gastric cancer lc-ms mtt assay |
| url | https://journals.lww.com/10.4103/jpbs.jpbs_631_24 |
| work_keys_str_mv | AT samirabdulkarimalharbi anticancerandantioxidantactivitiesofmethanolicextractofziziphusspinachristileavesagainsthumangastriccarcinomacells |