Anticancer and Antioxidant Activities of Methanolic Extract of Ziziphus Spina-Christi Leaves against Human Gastric Carcinoma Cells
Background: Ziziphus spina-christi (Rhamnaceae) is employed in Middle Eastern countries for the treatment of different diseases. Earlier reports revealed that it is cytotoxic to oral cancer cells. Objective: The current study determined the antioxidant activity and the anticancer potential of methan...
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| Main Author: | |
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| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2024-12-01
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| Series: | Journal of Pharmacy and Bioallied Sciences |
| Subjects: | |
| Online Access: | https://journals.lww.com/10.4103/jpbs.jpbs_631_24 |
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| Summary: | Background:
Ziziphus spina-christi (Rhamnaceae) is employed in Middle Eastern countries for the treatment of different diseases. Earlier reports revealed that it is cytotoxic to oral cancer cells.
Objective:
The current study determined the antioxidant activity and the anticancer potential of methanolic extract of Z. spina-christi against gastric carcinoma.
Materials and Methods:
LC-MS analysis was used to determine the different phytoconstituents predicted to be present in the extract. The effect of the Z. spina-christi leaf extract against human gastric carcinoma (AGS) cell lines was evaluated using MTT assay and cellular apoptosis techniques. A ferric-reducing antioxidant power assay was used to assess the antioxidant effect.
Results and Conclusion:
The LC-MS analysis revealed 24 phytoconstituents and Z. spina-christi extract was cytotoxic on AGS cells with an IC50 value of 125.1 μg/ml. Incubation of AGS cells with the extract decreased the percentage of live cells with a significant increase in late apoptotic phase and necrotic phase in the cellular apoptosis assay. The Z. spina-christi extract showed a potent antioxidant effect. The results show that Z. spina-christi extract possesses cytotoxic and antioxidant actions. |
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| ISSN: | 0976-4879 0975-7406 |