Experimental infectivity of Theileria luwenshuni and Theileria uilenbergi in Chinese Kunming mice

Theileria luwenshuni and Theileria uilenbergi are important tick-borne pathogens and cause substantial losses to the sheep industry in China. The improvement in detection techniques has allowed the identification of multi-homing parasitism in Theileria parasites. Herein we evaluated the experimental...

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Main Authors: You-quan LI, Peng-fei GUO, Jun-long LIU, Zhi-jie LIU, Yuan HAN, Xuan LI, Ai-hong LIU, Gui-quan GUAN, Guang-yuan LIU, Jian-xun LUO, Hong YIN
Format: Article
Language:English
Published: KeAi Communications Co., Ltd. 2018-02-01
Series:Journal of Integrative Agriculture
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Online Access:http://www.sciencedirect.com/science/article/pii/S2095311917618124
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author You-quan LI
Peng-fei GUO
Jun-long LIU
Zhi-jie LIU
Yuan HAN
Xuan LI
Ai-hong LIU
Gui-quan GUAN
Guang-yuan LIU
Jian-xun LUO
Hong YIN
author_facet You-quan LI
Peng-fei GUO
Jun-long LIU
Zhi-jie LIU
Yuan HAN
Xuan LI
Ai-hong LIU
Gui-quan GUAN
Guang-yuan LIU
Jian-xun LUO
Hong YIN
author_sort You-quan LI
collection DOAJ
description Theileria luwenshuni and Theileria uilenbergi are important tick-borne pathogens and cause substantial losses to the sheep industry in China. The improvement in detection techniques has allowed the identification of multi-homing parasitism in Theileria parasites. Herein we evaluated the experimental infectivity of T. luwenshuni and T. uilenbergi in Chinese Kunming mice by screening blood samples of experimentally inoculated mice by microscopic examination (ME) and PCR. T. luwenshuni infected Chinese Kunming mice and 20 mice inoculated with this parasite were positive by ME and PCR. In addition, T. uilenbergi infected mice and 20 mice inoculated with this species were positive by ME and PCR. However, the number of red blood cells and the levels of hemoglobin of 40 infected mice had no obvious changes in the course of infection. Our results demonstrated the multi-homing parasitism of T. luwenshuni and T. uilenbergi, which were believed to be parasites of sheep and goats. This study was the first to demonstrate the infection of T. luwenshuni and T. uilenbergi in Kunming mice.
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publisher KeAi Communications Co., Ltd.
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series Journal of Integrative Agriculture
spelling doaj-art-7c571152eaae48c0be87e7f06d9afa2d2025-08-20T03:56:59ZengKeAi Communications Co., Ltd.Journal of Integrative Agriculture2095-31192018-02-0117248849210.1016/S2095-3119(17)61812-4Experimental infectivity of Theileria luwenshuni and Theileria uilenbergi in Chinese Kunming miceYou-quan LI0Peng-fei GUO1Jun-long LIU2Zhi-jie LIU3Yuan HAN4Xuan LI5Ai-hong LIU6Gui-quan GUAN7Guang-yuan LIU8Jian-xun LUO9Hong YIN10State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P.R. China; LI You-quan, Tel: +86-931-8342515, Fax: +86-931-8340977State Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P.R. ChinaState Key Laboratory of Veterinary Etiological Biology/Key Laboratory of Veterinary Parasitology of Gansu Province, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, P.R. China; Jiangsu Co-Innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, P.R. China; Correspondence YIN HongTheileria luwenshuni and Theileria uilenbergi are important tick-borne pathogens and cause substantial losses to the sheep industry in China. The improvement in detection techniques has allowed the identification of multi-homing parasitism in Theileria parasites. Herein we evaluated the experimental infectivity of T. luwenshuni and T. uilenbergi in Chinese Kunming mice by screening blood samples of experimentally inoculated mice by microscopic examination (ME) and PCR. T. luwenshuni infected Chinese Kunming mice and 20 mice inoculated with this parasite were positive by ME and PCR. In addition, T. uilenbergi infected mice and 20 mice inoculated with this species were positive by ME and PCR. However, the number of red blood cells and the levels of hemoglobin of 40 infected mice had no obvious changes in the course of infection. Our results demonstrated the multi-homing parasitism of T. luwenshuni and T. uilenbergi, which were believed to be parasites of sheep and goats. This study was the first to demonstrate the infection of T. luwenshuni and T. uilenbergi in Kunming mice.http://www.sciencedirect.com/science/article/pii/S2095311917618124Theileria luwenshuniTheileria uilenbergiinfectionmousePCRmicroscopic examination
spellingShingle You-quan LI
Peng-fei GUO
Jun-long LIU
Zhi-jie LIU
Yuan HAN
Xuan LI
Ai-hong LIU
Gui-quan GUAN
Guang-yuan LIU
Jian-xun LUO
Hong YIN
Experimental infectivity of Theileria luwenshuni and Theileria uilenbergi in Chinese Kunming mice
Journal of Integrative Agriculture
Theileria luwenshuni
Theileria uilenbergi
infection
mouse
PCR
microscopic examination
title Experimental infectivity of Theileria luwenshuni and Theileria uilenbergi in Chinese Kunming mice
title_full Experimental infectivity of Theileria luwenshuni and Theileria uilenbergi in Chinese Kunming mice
title_fullStr Experimental infectivity of Theileria luwenshuni and Theileria uilenbergi in Chinese Kunming mice
title_full_unstemmed Experimental infectivity of Theileria luwenshuni and Theileria uilenbergi in Chinese Kunming mice
title_short Experimental infectivity of Theileria luwenshuni and Theileria uilenbergi in Chinese Kunming mice
title_sort experimental infectivity of theileria luwenshuni and theileria uilenbergi in chinese kunming mice
topic Theileria luwenshuni
Theileria uilenbergi
infection
mouse
PCR
microscopic examination
url http://www.sciencedirect.com/science/article/pii/S2095311917618124
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