Synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biochar

Bacteria capable of degrading oil spillage hydrocarbons are widely identified, yet single bacterial strains often underperform in real environments due to competition with native microorganisms, leading to decreased degradation efficiency. In this study, an eco-friendly approach was developed for re...

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Main Authors: Prithwindra Sarkar, S. Jitendra Pal
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:Environmental Challenges
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Online Access:http://www.sciencedirect.com/science/article/pii/S2667010025001696
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author Prithwindra Sarkar
S. Jitendra Pal
author_facet Prithwindra Sarkar
S. Jitendra Pal
author_sort Prithwindra Sarkar
collection DOAJ
description Bacteria capable of degrading oil spillage hydrocarbons are widely identified, yet single bacterial strains often underperform in real environments due to competition with native microorganisms, leading to decreased degradation efficiency. In this study, an eco-friendly approach was developed for removing hydrocarbons from aqueous systems by immobilizing consortium of Novosphingobium sp. MCC 3051 and Alcanivorax sp. MCC 4337 onto rice husk-derived biochar (RHB). The Hydrocarbons remediation process was systematically optimized using response surface methodology (RSM). MCC 3051 and MCC 4337 exhibited a highly hydrophobic cell surface. Genetic analysis revealed multiple metabolic pathways for breaking down both alkanes and aromatic hydrocarbons. Experimentally, MCC 3051 and MCC 4337 completely degraded short-chain Hydrocarbons (C10–C22) and achieved over 97% degradation within 7 days. These findings suggest MCC 3051 and MCC 4337 hold strong promise for remediating oil-contaminated water due to its broad degradation range and high environmental adaptability. Gas Chromatography (GC) analyses identified the degradation products, signifying a synergistic effect of bacterial metabolism and adsorption in the Hydrocarbons removal mechanism. These findings highlight the potential of immobilized MCC 3051, MCC 4337 and Consortium loaded rice husk biochar (CRHB) for efficient remediation of marine oil-contaminated environments.
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spelling doaj-art-7c4b0294a6bd4a1a9d0c05a43c7e004a2025-08-20T03:43:55ZengElsevierEnvironmental Challenges2667-01002025-09-012010125010.1016/j.envc.2025.101250Synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biocharPrithwindra Sarkar0S. Jitendra Pal1Department of Chemical Engineering, National Institute of Technology Karnataka (NITK), Surathkal, Srinivasanagar P.O., Mangalore 575025, IndiaCorresponding author at: Department of Chemical Engineering, National Institute of Technology Karnataka (NITK), Surathkal, India; Department of Chemical Engineering, National Institute of Technology Karnataka (NITK), Surathkal, Srinivasanagar P.O., Mangalore 575025, IndiaBacteria capable of degrading oil spillage hydrocarbons are widely identified, yet single bacterial strains often underperform in real environments due to competition with native microorganisms, leading to decreased degradation efficiency. In this study, an eco-friendly approach was developed for removing hydrocarbons from aqueous systems by immobilizing consortium of Novosphingobium sp. MCC 3051 and Alcanivorax sp. MCC 4337 onto rice husk-derived biochar (RHB). The Hydrocarbons remediation process was systematically optimized using response surface methodology (RSM). MCC 3051 and MCC 4337 exhibited a highly hydrophobic cell surface. Genetic analysis revealed multiple metabolic pathways for breaking down both alkanes and aromatic hydrocarbons. Experimentally, MCC 3051 and MCC 4337 completely degraded short-chain Hydrocarbons (C10–C22) and achieved over 97% degradation within 7 days. These findings suggest MCC 3051 and MCC 4337 hold strong promise for remediating oil-contaminated water due to its broad degradation range and high environmental adaptability. Gas Chromatography (GC) analyses identified the degradation products, signifying a synergistic effect of bacterial metabolism and adsorption in the Hydrocarbons removal mechanism. These findings highlight the potential of immobilized MCC 3051, MCC 4337 and Consortium loaded rice husk biochar (CRHB) for efficient remediation of marine oil-contaminated environments.http://www.sciencedirect.com/science/article/pii/S2667010025001696BioremediationRice husk biochar (RHB)Microbial immobilizationConsortium loaded Rice husk biochar (CRHB)Consortium immobilized Rice husk biochar after treatment (CRHBT)Extracellular polymeric substances (EPS)
spellingShingle Prithwindra Sarkar
S. Jitendra Pal
Synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biochar
Environmental Challenges
Bioremediation
Rice husk biochar (RHB)
Microbial immobilization
Consortium loaded Rice husk biochar (CRHB)
Consortium immobilized Rice husk biochar after treatment (CRHBT)
Extracellular polymeric substances (EPS)
title Synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biochar
title_full Synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biochar
title_fullStr Synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biochar
title_full_unstemmed Synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biochar
title_short Synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biochar
title_sort synergistic bioremediation of marine oil spills using dual strain consortium immobilized rice husk biochar
topic Bioremediation
Rice husk biochar (RHB)
Microbial immobilization
Consortium loaded Rice husk biochar (CRHB)
Consortium immobilized Rice husk biochar after treatment (CRHBT)
Extracellular polymeric substances (EPS)
url http://www.sciencedirect.com/science/article/pii/S2667010025001696
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