Oncostatin M [OSM] protein immuno-expression in oral squamous cell carcinoma – A prospective study
Background: An important factor in the progression of tumours is the formation of blood vessels in the tumour stroma. When there is an upregulation of angiogenesis stimulators or a downregulation of angiogenesis inhibitors, the tumour may manifest its capacity for angiogenesis. Immune cells secrete...
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| Language: | English |
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Elsevier
2024-06-01
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| Series: | Oral Oncology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772906024002218 |
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| author | Sunil Prakash Nandhini Gunasekaran Ramya Mahalingam Rajkumar Krishnan |
| author_facet | Sunil Prakash Nandhini Gunasekaran Ramya Mahalingam Rajkumar Krishnan |
| author_sort | Sunil Prakash |
| collection | DOAJ |
| description | Background: An important factor in the progression of tumours is the formation of blood vessels in the tumour stroma. When there is an upregulation of angiogenesis stimulators or a downregulation of angiogenesis inhibitors, the tumour may manifest its capacity for angiogenesis. Immune cells secrete a substance called Oncostatin M (OSM), which has been linked to cancer angiogenesis and subsequent pathogenesis. This study is aimed to assess OSM expression in oral squamous cell carcinoma. Objective: To compare the intensity of expression of Oncostatin M [OSM] in normal oral mucosa and Oral Squamous Cell Carcinoma and to analyse their role in the disease progression by enhancing angiogenesis and metastasis. Materials and methods: The study analysed the expression of OSM with the immunohistochemical aid. The samples were treated with Anti-OSM polyclonal antibody and immunohistochemical staining was done using NovoLinkTM NovocastraTM Polymer Detection System (Leica Microsystems). Stained samples were categorized based on the staining percentage and staining intensity. Results: The comparison of staining percentage between the epithelium of control group and the OSCC group revealed that it was statistically significant [p value = 0.002685]. Similarly, the comparison of staining intensity [p value = 0.001202] and staining index [p value = 0.001817] between the control group and the OSCC group revealed that it was statistically significant. Conclusion: The expression of oncostatin M in normal epithelium and OSCC group with the given immunohistochemical staining percentage, intensity and staining index suggests strong diagnostic potential of oncostatin M which can also be used for further therapeutic. |
| format | Article |
| id | doaj-art-7c44a3d1fb434b65b3163c85c47b3c75 |
| institution | Kabale University |
| issn | 2772-9060 |
| language | English |
| publishDate | 2024-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Oral Oncology Reports |
| spelling | doaj-art-7c44a3d1fb434b65b3163c85c47b3c752025-01-09T06:16:00ZengElsevierOral Oncology Reports2772-90602024-06-0110100375Oncostatin M [OSM] protein immuno-expression in oral squamous cell carcinoma – A prospective studySunil Prakash0Nandhini Gunasekaran1Ramya Mahalingam2Rajkumar Krishnan3Department of Oral Pathology & Microbiology, SRM Dental College, Ramapuram, Chennai, 600089, IndiaCorresponding author. Department of Oral Pathology & Microbiology, SRM Dental College, Bharathi Salai, Ramapuram, Chennai, 600089, Tamil Nadu, India.; Department of Oral Pathology & Microbiology, SRM Dental College, Ramapuram, Chennai, 600089, IndiaDepartment of Oral Pathology & Microbiology, SRM Dental College, Ramapuram, Chennai, 600089, IndiaDepartment of Oral Pathology & Microbiology, SRM Dental College, Ramapuram, Chennai, 600089, IndiaBackground: An important factor in the progression of tumours is the formation of blood vessels in the tumour stroma. When there is an upregulation of angiogenesis stimulators or a downregulation of angiogenesis inhibitors, the tumour may manifest its capacity for angiogenesis. Immune cells secrete a substance called Oncostatin M (OSM), which has been linked to cancer angiogenesis and subsequent pathogenesis. This study is aimed to assess OSM expression in oral squamous cell carcinoma. Objective: To compare the intensity of expression of Oncostatin M [OSM] in normal oral mucosa and Oral Squamous Cell Carcinoma and to analyse their role in the disease progression by enhancing angiogenesis and metastasis. Materials and methods: The study analysed the expression of OSM with the immunohistochemical aid. The samples were treated with Anti-OSM polyclonal antibody and immunohistochemical staining was done using NovoLinkTM NovocastraTM Polymer Detection System (Leica Microsystems). Stained samples were categorized based on the staining percentage and staining intensity. Results: The comparison of staining percentage between the epithelium of control group and the OSCC group revealed that it was statistically significant [p value = 0.002685]. Similarly, the comparison of staining intensity [p value = 0.001202] and staining index [p value = 0.001817] between the control group and the OSCC group revealed that it was statistically significant. Conclusion: The expression of oncostatin M in normal epithelium and OSCC group with the given immunohistochemical staining percentage, intensity and staining index suggests strong diagnostic potential of oncostatin M which can also be used for further therapeutic.http://www.sciencedirect.com/science/article/pii/S2772906024002218Oncostatin MAngiogenesisTumor invasionInterleukins and tumor stroma |
| spellingShingle | Sunil Prakash Nandhini Gunasekaran Ramya Mahalingam Rajkumar Krishnan Oncostatin M [OSM] protein immuno-expression in oral squamous cell carcinoma – A prospective study Oral Oncology Reports Oncostatin M Angiogenesis Tumor invasion Interleukins and tumor stroma |
| title | Oncostatin M [OSM] protein immuno-expression in oral squamous cell carcinoma – A prospective study |
| title_full | Oncostatin M [OSM] protein immuno-expression in oral squamous cell carcinoma – A prospective study |
| title_fullStr | Oncostatin M [OSM] protein immuno-expression in oral squamous cell carcinoma – A prospective study |
| title_full_unstemmed | Oncostatin M [OSM] protein immuno-expression in oral squamous cell carcinoma – A prospective study |
| title_short | Oncostatin M [OSM] protein immuno-expression in oral squamous cell carcinoma – A prospective study |
| title_sort | oncostatin m osm protein immuno expression in oral squamous cell carcinoma a prospective study |
| topic | Oncostatin M Angiogenesis Tumor invasion Interleukins and tumor stroma |
| url | http://www.sciencedirect.com/science/article/pii/S2772906024002218 |
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