HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE
Objective. Investigation of hypolipidemic effect of sesquiterpene γ-lactone Arglabin in hepatoma tissue culture (HTC).Materials and methods. In this study we’ve evaluated the effect of sesquiterpene γ-lactone Arglabin and gemfibrozil (reference drug) on the lipid content in the hepatoma tissue cultu...
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Siberian State Medical University (Tomsk)
2015-12-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/381 |
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| author | A. V. Ratkin Yu. A. Pfarger V. V. Ivanov S. M. Adekenov O. A. Kaidash V. S. Chuchalin V. V. Novitsky |
| author_facet | A. V. Ratkin Yu. A. Pfarger V. V. Ivanov S. M. Adekenov O. A. Kaidash V. S. Chuchalin V. V. Novitsky |
| author_sort | A. V. Ratkin |
| collection | DOAJ |
| description | Objective. Investigation of hypolipidemic effect of sesquiterpene γ-lactone Arglabin in hepatoma tissue culture (HTC).Materials and methods. In this study we’ve evaluated the effect of sesquiterpene γ-lactone Arglabin and gemfibrozil (reference drug) on the lipid content in the hepatoma tissue culture (HTC) which were incubated with a fat emulsion “Lipofundin” by fluorescent method with vital dye Nile Red. The cell viability was investigated using the MTT-test and staining by Trypan blue.Results. Cultivation of cell cultures of rat’s hepatoma cell line HTC with Arglabin and gemfibrozil in concentrations from 10 to 50 μmol and from 0.25 to 0.5 mmol, respectively, had no cytotoxic effect. HTC cell viability did not change compared with the corresponding rate in the control culture. Experimental hyperlipidemia in hepatoma culture was induced by the addition in the incubation medium of fat emulsion “Lipofundin” in a final concentration of 0.05 %. The fluorescence intensity of Nile Red in the cells was increased 4-fold (p < 0.05), which indicates a significant accumulation of lipids in the cytosol of cells. In these steady-state Arglabin and gemfibrozil at concentrations 75–100 μM and 0.25–1.0 mM, respectively, reduced the content of lipid in cells. Conclusion. In the model of hyperlipidemia induced by lipofundin, sesquiterpene γ-lactone Arglabin prevents the accumulation of lipids in the HTC cell line, as evidenced by a decrease in Nile Red fluorescence. However hypolipidemic effect of Arglabin is associated with cytotoxic effects, which is typical for anticancer drugs. |
| format | Article |
| id | doaj-art-7bda0113a8904bbd9ddb25c2b958bea8 |
| institution | Kabale University |
| issn | 1682-0363 1819-3684 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj-art-7bda0113a8904bbd9ddb25c2b958bea82025-08-20T04:00:12ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842015-12-01146758010.20538/1682-0363-2015-6-75-80368HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTUREA. V. Ratkin0Yu. A. Pfarger1V. V. Ivanov2S. M. Adekenov3O. A. Kaidash4V. S. Chuchalin5V. V. Novitsky6Siberian State Medical University, Tomsk, Russian FederationSiberian State Medical University, Tomsk, Russian FederationSiberian State Medical University, Tomsk, Russian FederationInternational Scientific-Industrial Holding “Phytochemistry”, Karaganda, KazakhstanSiberian State Medical University, Tomsk, Russian FederationSiberian State Medical University, Tomsk, Russian FederationSiberian State Medical University, Tomsk, Russian FederationObjective. Investigation of hypolipidemic effect of sesquiterpene γ-lactone Arglabin in hepatoma tissue culture (HTC).Materials and methods. In this study we’ve evaluated the effect of sesquiterpene γ-lactone Arglabin and gemfibrozil (reference drug) on the lipid content in the hepatoma tissue culture (HTC) which were incubated with a fat emulsion “Lipofundin” by fluorescent method with vital dye Nile Red. The cell viability was investigated using the MTT-test and staining by Trypan blue.Results. Cultivation of cell cultures of rat’s hepatoma cell line HTC with Arglabin and gemfibrozil in concentrations from 10 to 50 μmol and from 0.25 to 0.5 mmol, respectively, had no cytotoxic effect. HTC cell viability did not change compared with the corresponding rate in the control culture. Experimental hyperlipidemia in hepatoma culture was induced by the addition in the incubation medium of fat emulsion “Lipofundin” in a final concentration of 0.05 %. The fluorescence intensity of Nile Red in the cells was increased 4-fold (p < 0.05), which indicates a significant accumulation of lipids in the cytosol of cells. In these steady-state Arglabin and gemfibrozil at concentrations 75–100 μM and 0.25–1.0 mM, respectively, reduced the content of lipid in cells. Conclusion. In the model of hyperlipidemia induced by lipofundin, sesquiterpene γ-lactone Arglabin prevents the accumulation of lipids in the HTC cell line, as evidenced by a decrease in Nile Red fluorescence. However hypolipidemic effect of Arglabin is associated with cytotoxic effects, which is typical for anticancer drugs.https://bulletin.ssmu.ru/jour/article/view/381sesquiterpene γ-lactone arglabingemfibrozilhepatoma tissue culture (htc)hypolipidemic effect |
| spellingShingle | A. V. Ratkin Yu. A. Pfarger V. V. Ivanov S. M. Adekenov O. A. Kaidash V. S. Chuchalin V. V. Novitsky HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE Бюллетень сибирской медицины sesquiterpene γ-lactone arglabin gemfibrozil hepatoma tissue culture (htc) hypolipidemic effect |
| title | HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE |
| title_full | HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE |
| title_fullStr | HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE |
| title_full_unstemmed | HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE |
| title_short | HYPOLIPIDEMIC EFFECT OF ARGLABIN IN HEPATOMA TISSUE CULTURE |
| title_sort | hypolipidemic effect of arglabin in hepatoma tissue culture |
| topic | sesquiterpene γ-lactone arglabin gemfibrozil hepatoma tissue culture (htc) hypolipidemic effect |
| url | https://bulletin.ssmu.ru/jour/article/view/381 |
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