A single-plasmid-based, easily curable CRISPR/Cas9 system for rapid, iterative genome editing in Pseudomonas putida KT2440
Abstract Background Pseudomonas putida KT2440, a non-pathogenic soil bacterium, is a key platform strain in synthetic biology and industrial applications due to its robustness and metabolic versatility. Various systems have been developed for genome editing in P. putida, including transposon modules...
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Main Authors: | Qifeng Wen, JinJin Chen, Jin Li, Ida Putu Wiweka Dharmasiddhi, Maohua Yang, Jianmin Xing, Yilan Liu |
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Format: | Article |
Language: | English |
Published: |
BMC
2024-12-01
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Series: | Microbial Cell Factories |
Subjects: | |
Online Access: | https://doi.org/10.1186/s12934-024-02634-4 |
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