Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software
Summary: Here, we present a protocol for using Myotally, a user-friendly software for fast, automated quantification of muscle fiber size, number, and central nucleation from immunofluorescent stains of mouse skeletal muscle cross-sections. We describe steps for installing the software, preparing co...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-03-01
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| Series: | STAR Protocols |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166724007202 |
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| author | Pieter Both Soochi Kim Jengmin Kang Marina Arjona Daniel I. Benjamin Christopher W. Nutter Armon Goshayeshi Thomas A. Rando |
| author_facet | Pieter Both Soochi Kim Jengmin Kang Marina Arjona Daniel I. Benjamin Christopher W. Nutter Armon Goshayeshi Thomas A. Rando |
| author_sort | Pieter Both |
| collection | DOAJ |
| description | Summary: Here, we present a protocol for using Myotally, a user-friendly software for fast, automated quantification of muscle fiber size, number, and central nucleation from immunofluorescent stains of mouse skeletal muscle cross-sections. We describe steps for installing the software, preparing compatible images, finding the file path, and selecting key parameters like image quality and size limits. We also detail optional features, such as measuring mean fluorescence. By automating these traditionally labor-intensive processes, Myotally improves research efficiency and data consistency. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics. |
| format | Article |
| id | doaj-art-7b650e05d46e4aa798ab847c6ecb5230 |
| institution | Kabale University |
| issn | 2666-1667 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | STAR Protocols |
| spelling | doaj-art-7b650e05d46e4aa798ab847c6ecb52302025-01-12T05:25:46ZengElsevierSTAR Protocols2666-16672025-03-0161103555Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally softwarePieter Both0Soochi Kim1Jengmin Kang2Marina Arjona3Daniel I. Benjamin4Christopher W. Nutter5Armon Goshayeshi6Thomas A. Rando7Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA; Stem Cell Biology and Regenerative Medicine Graduate Program, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA; Department of Biotechnology and Bioinformatics, Korea University, Sejong 30019, Republic of KoreaDepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USADepartment of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA 94305, USA; Neurology Service, Veterans Affairs Palo Alto Health Care System, Palo Alto, CA 94304, USA; Corresponding authorSummary: Here, we present a protocol for using Myotally, a user-friendly software for fast, automated quantification of muscle fiber size, number, and central nucleation from immunofluorescent stains of mouse skeletal muscle cross-sections. We describe steps for installing the software, preparing compatible images, finding the file path, and selecting key parameters like image quality and size limits. We also detail optional features, such as measuring mean fluorescence. By automating these traditionally labor-intensive processes, Myotally improves research efficiency and data consistency. : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.http://www.sciencedirect.com/science/article/pii/S2666166724007202Cell BiologyMicroscopyComputer sciences |
| spellingShingle | Pieter Both Soochi Kim Jengmin Kang Marina Arjona Daniel I. Benjamin Christopher W. Nutter Armon Goshayeshi Thomas A. Rando Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software STAR Protocols Cell Biology Microscopy Computer sciences |
| title | Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software |
| title_full | Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software |
| title_fullStr | Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software |
| title_full_unstemmed | Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software |
| title_short | Protocol for quantifying muscle fiber size, number, and central nucleation of mouse skeletal muscle cross-sections using Myotally software |
| title_sort | protocol for quantifying muscle fiber size number and central nucleation of mouse skeletal muscle cross sections using myotally software |
| topic | Cell Biology Microscopy Computer sciences |
| url | http://www.sciencedirect.com/science/article/pii/S2666166724007202 |
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