Involvement of a 1-Cys peroxiredoxin in bacterial virulence.
The killing of bacterial pathogens by macrophages occurs via the oxidative burst and bacteria have evolved to overcome this challenge and survive, using several virulence and defense strategies, including antioxidant mechanisms. We show here that the 1-Cys peroxiredoxin LsfA from the opportunistic p...
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-10-01
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| Series: | PLoS Pathogens |
| Online Access: | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1004442&type=printable |
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| author | Gilberto Hideo Kaihami José Roberto Fogaça de Almeida Suelen Silvana dos Santos Luis Eduardo Soares Netto Sandro Rogério de Almeida Regina Lúcia Baldini |
| author_facet | Gilberto Hideo Kaihami José Roberto Fogaça de Almeida Suelen Silvana dos Santos Luis Eduardo Soares Netto Sandro Rogério de Almeida Regina Lúcia Baldini |
| author_sort | Gilberto Hideo Kaihami |
| collection | DOAJ |
| description | The killing of bacterial pathogens by macrophages occurs via the oxidative burst and bacteria have evolved to overcome this challenge and survive, using several virulence and defense strategies, including antioxidant mechanisms. We show here that the 1-Cys peroxiredoxin LsfA from the opportunistic pathogen Pseudomonas aeruginosa is endowed with thiol-dependent peroxidase activity that protects the bacteria from H(2)O(2) and that this protein is implicated in pathogenicity. LsfA belongs to the poorly studied Prx6 subfamily of peroxiredoxins. The function of these peroxiredoxins has not been characterized in bacteria, and their contribution to host-pathogen interactions remains unknown. Infection of macrophages with the lsfA mutant strains resulted in higher levels of the cytokine TNF-α production due to the activation of the NF-kB and MAPK pathways, that are partially inhibited by the wild-type P. aeruginosa strain. A redox fluorescent probe was more oxidized in the lsfA mutant-infected macrophages than it was in the macrophages infected with the wild-type strain, suggesting that the oxidative burst was overstimulated in the absence of LsfA. Although no differences in the phagocytosis rates were observed when macrophages were infected with wild-type and mutant bacteria in a gentamicin exclusion assay, a higher number of wild-type bacterial cells was found in the supernatant. This difference was not observed when macrophages were pre-treated with a NADPH oxidase inhibitor, confirming the role of LsfA in the bacterial resistance to ROS generated via NADPH oxidase. In an acute pneumonia model, mice infected with the mutant strains presented higher cytokine release in the lungs and increased activated neutrophil recruitment, with reduced bacterial burden and improved survival rates compared to mice infected with the wild-type bacteria. LsfA is the first bacterial 1-Cys Prx shown to modulate host immune responses and its characterization will allow a better understanding of the role of redox signaling in host-pathogen interactions. |
| format | Article |
| id | doaj-art-7b46e67ff76344969f2327714dd33681 |
| institution | Kabale University |
| issn | 1553-7366 1553-7374 |
| language | English |
| publishDate | 2014-10-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS Pathogens |
| spelling | doaj-art-7b46e67ff76344969f2327714dd336812025-01-16T05:30:58ZengPublic Library of Science (PLoS)PLoS Pathogens1553-73661553-73742014-10-011010e100444210.1371/journal.ppat.1004442Involvement of a 1-Cys peroxiredoxin in bacterial virulence.Gilberto Hideo KaihamiJosé Roberto Fogaça de AlmeidaSuelen Silvana dos SantosLuis Eduardo Soares NettoSandro Rogério de AlmeidaRegina Lúcia BaldiniThe killing of bacterial pathogens by macrophages occurs via the oxidative burst and bacteria have evolved to overcome this challenge and survive, using several virulence and defense strategies, including antioxidant mechanisms. We show here that the 1-Cys peroxiredoxin LsfA from the opportunistic pathogen Pseudomonas aeruginosa is endowed with thiol-dependent peroxidase activity that protects the bacteria from H(2)O(2) and that this protein is implicated in pathogenicity. LsfA belongs to the poorly studied Prx6 subfamily of peroxiredoxins. The function of these peroxiredoxins has not been characterized in bacteria, and their contribution to host-pathogen interactions remains unknown. Infection of macrophages with the lsfA mutant strains resulted in higher levels of the cytokine TNF-α production due to the activation of the NF-kB and MAPK pathways, that are partially inhibited by the wild-type P. aeruginosa strain. A redox fluorescent probe was more oxidized in the lsfA mutant-infected macrophages than it was in the macrophages infected with the wild-type strain, suggesting that the oxidative burst was overstimulated in the absence of LsfA. Although no differences in the phagocytosis rates were observed when macrophages were infected with wild-type and mutant bacteria in a gentamicin exclusion assay, a higher number of wild-type bacterial cells was found in the supernatant. This difference was not observed when macrophages were pre-treated with a NADPH oxidase inhibitor, confirming the role of LsfA in the bacterial resistance to ROS generated via NADPH oxidase. In an acute pneumonia model, mice infected with the mutant strains presented higher cytokine release in the lungs and increased activated neutrophil recruitment, with reduced bacterial burden and improved survival rates compared to mice infected with the wild-type bacteria. LsfA is the first bacterial 1-Cys Prx shown to modulate host immune responses and its characterization will allow a better understanding of the role of redox signaling in host-pathogen interactions.https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1004442&type=printable |
| spellingShingle | Gilberto Hideo Kaihami José Roberto Fogaça de Almeida Suelen Silvana dos Santos Luis Eduardo Soares Netto Sandro Rogério de Almeida Regina Lúcia Baldini Involvement of a 1-Cys peroxiredoxin in bacterial virulence. PLoS Pathogens |
| title | Involvement of a 1-Cys peroxiredoxin in bacterial virulence. |
| title_full | Involvement of a 1-Cys peroxiredoxin in bacterial virulence. |
| title_fullStr | Involvement of a 1-Cys peroxiredoxin in bacterial virulence. |
| title_full_unstemmed | Involvement of a 1-Cys peroxiredoxin in bacterial virulence. |
| title_short | Involvement of a 1-Cys peroxiredoxin in bacterial virulence. |
| title_sort | involvement of a 1 cys peroxiredoxin in bacterial virulence |
| url | https://journals.plos.org/plospathogens/article/file?id=10.1371/journal.ppat.1004442&type=printable |
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