Molecular dissection of hemizygote-dependent dominance of super-early flowering in soybean
In plants, numerous non-Mendelian inherited dominant effects, including over-, incomplete-, and co-dominance, are frequently observed, yet they remain insufficiently understood. A novel phenotype has been identified in specific soybean transformants overexpressing a single 35S::GmFT2a copy: super-ea...
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| Language: | English |
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KeAi Communications Co., Ltd.
2025-06-01
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| Series: | Crop Journal |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2214514125000583 |
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| author | Xin Xu Yang Yu Bingjun Jiang Dong Cao Lixin Zhang Hongchang Jia Xuegang Sun Li Chen Shan Yuan Fulu Chen Zefu Lu Yanhong Liu Mahmoud Naser Tingting Wu Cunxiang Wu Qingzhu Zhang Shi Sun Tianfu Han |
| author_facet | Xin Xu Yang Yu Bingjun Jiang Dong Cao Lixin Zhang Hongchang Jia Xuegang Sun Li Chen Shan Yuan Fulu Chen Zefu Lu Yanhong Liu Mahmoud Naser Tingting Wu Cunxiang Wu Qingzhu Zhang Shi Sun Tianfu Han |
| author_sort | Xin Xu |
| collection | DOAJ |
| description | In plants, numerous non-Mendelian inherited dominant effects, including over-, incomplete-, and co-dominance, are frequently observed, yet they remain insufficiently understood. A novel phenotype has been identified in specific soybean transformants overexpressing a single 35S::GmFT2a copy: super-early flowering dominance is exclusively observed in hemizygotes, not in homozygotes. Homozygous individual exhibits siRNA-mediated DNA methylation, causing epigenetic transcriptional silencing, whereas no such effect occurs in hemizygotes. Intriguingly, two distinct rounds of DNA methylation establishment occur, each mediated by a different mechanism. The homozygotes that derived from the hemizygous mother plants carrying 35S::GmFT2a locus was associated with the initiation of CHH-context DNA methylation at 35S promoters mediated by 21 and 22 nucleotide (nt) siRNAs. Subsequently, 24 nt siRNAs contribute to additional CHG- and CG-context DNA methylation at 35S promoters during the homozygosity of genes in plants already homozygous in maternal lineage. Reducing DNA methylation levels can be achieved by generating a hemizygous genotype through a crossing experiment with a recessive genotype. This research has unveiled a phenomenon: hemizygote-dependent dominance resulting from transcriptional silencing in homozygote offsprings. It provides new insights into the molecular mechanism underlying dominant effects. |
| format | Article |
| id | doaj-art-7ada5ac53b5b45f9b3bfc6b6f1f51e4f |
| institution | Kabale University |
| issn | 2214-5141 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Crop Journal |
| spelling | doaj-art-7ada5ac53b5b45f9b3bfc6b6f1f51e4f2025-08-20T03:47:13ZengKeAi Communications Co., Ltd.Crop Journal2214-51412025-06-0113382883910.1016/j.cj.2025.02.007Molecular dissection of hemizygote-dependent dominance of super-early flowering in soybeanXin Xu0Yang Yu1Bingjun Jiang2Dong Cao3Lixin Zhang4Hongchang Jia5Xuegang Sun6Li Chen7Shan Yuan8Fulu Chen9Zefu Lu10Yanhong Liu11Mahmoud Naser12Tingting Wu13Cunxiang Wu14Qingzhu Zhang15Shi Sun16Tianfu Han17State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Beijing Key Laboratory of Agricultural Genetic Resources and Biotechnology, Institute of Biotechnology, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Oil Crops Research Institute, Chinese Academy of Agricultural Sciences, Wuhan 430062, Hubei, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, ChinaCollege of Life Sciences, Northeast Forestry University, Harbin 150040, Heilongjiang, ChinaState Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; Corresponding authors.State Key Laboratory of Crop Gene Resources and Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China; National Nanfan Research Institute, Chinese Academy of Agricultural Sciences, Sanya 572000, Hainan, China; Corresponding authors.In plants, numerous non-Mendelian inherited dominant effects, including over-, incomplete-, and co-dominance, are frequently observed, yet they remain insufficiently understood. A novel phenotype has been identified in specific soybean transformants overexpressing a single 35S::GmFT2a copy: super-early flowering dominance is exclusively observed in hemizygotes, not in homozygotes. Homozygous individual exhibits siRNA-mediated DNA methylation, causing epigenetic transcriptional silencing, whereas no such effect occurs in hemizygotes. Intriguingly, two distinct rounds of DNA methylation establishment occur, each mediated by a different mechanism. The homozygotes that derived from the hemizygous mother plants carrying 35S::GmFT2a locus was associated with the initiation of CHH-context DNA methylation at 35S promoters mediated by 21 and 22 nucleotide (nt) siRNAs. Subsequently, 24 nt siRNAs contribute to additional CHG- and CG-context DNA methylation at 35S promoters during the homozygosity of genes in plants already homozygous in maternal lineage. Reducing DNA methylation levels can be achieved by generating a hemizygous genotype through a crossing experiment with a recessive genotype. This research has unveiled a phenomenon: hemizygote-dependent dominance resulting from transcriptional silencing in homozygote offsprings. It provides new insights into the molecular mechanism underlying dominant effects.http://www.sciencedirect.com/science/article/pii/S2214514125000583SoybeanHemizygote-dependent dominanceFlowering timesiRNADNA methylation |
| spellingShingle | Xin Xu Yang Yu Bingjun Jiang Dong Cao Lixin Zhang Hongchang Jia Xuegang Sun Li Chen Shan Yuan Fulu Chen Zefu Lu Yanhong Liu Mahmoud Naser Tingting Wu Cunxiang Wu Qingzhu Zhang Shi Sun Tianfu Han Molecular dissection of hemizygote-dependent dominance of super-early flowering in soybean Crop Journal Soybean Hemizygote-dependent dominance Flowering time siRNA DNA methylation |
| title | Molecular dissection of hemizygote-dependent dominance of super-early flowering in soybean |
| title_full | Molecular dissection of hemizygote-dependent dominance of super-early flowering in soybean |
| title_fullStr | Molecular dissection of hemizygote-dependent dominance of super-early flowering in soybean |
| title_full_unstemmed | Molecular dissection of hemizygote-dependent dominance of super-early flowering in soybean |
| title_short | Molecular dissection of hemizygote-dependent dominance of super-early flowering in soybean |
| title_sort | molecular dissection of hemizygote dependent dominance of super early flowering in soybean |
| topic | Soybean Hemizygote-dependent dominance Flowering time siRNA DNA methylation |
| url | http://www.sciencedirect.com/science/article/pii/S2214514125000583 |
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