Melatonin induces drought stress tolerance by regulating the physiological mechanisms, antioxidant enzymes, and leaf structural modifications in Rosa centifolia L
Melatonin is considered an effective bio-stimulant that is crucial in managing several abiotic stresses including drought. However, its potential mechanisms against drought stress in fragrant roses are not well understood. Here, we aim to investigate the role of melatonin on Rosa centifolia plants c...
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Elsevier
2025-01-01
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| Series: | Heliyon |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844024172672 |
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| author | Muhammad Ahsan Adnan Younis Aftab Jamal Mohammed O. Alshaharni Uthman Balgith Algopishi Abeer Al-Andal Mateen Sajid Muhammad Naeem Jawad Ahmad Khan Emanuele Radicetti Mohammad Valipour Gulzar Akhtar |
| author_facet | Muhammad Ahsan Adnan Younis Aftab Jamal Mohammed O. Alshaharni Uthman Balgith Algopishi Abeer Al-Andal Mateen Sajid Muhammad Naeem Jawad Ahmad Khan Emanuele Radicetti Mohammad Valipour Gulzar Akhtar |
| author_sort | Muhammad Ahsan |
| collection | DOAJ |
| description | Melatonin is considered an effective bio-stimulant that is crucial in managing several abiotic stresses including drought. However, its potential mechanisms against drought stress in fragrant roses are not well understood. Here, we aim to investigate the role of melatonin on Rosa centifolia plants cultivated under drought stress (40 % field capacity) and normal irrigation (80 % field capacity). Plant growth traits, gaseous exchange, antioxidants, osmolytes, oxidative stress, and leaf anatomical attributes were measured. All pots were arranged with a completely randomized design with two-factor factorial setup. Foliar application of melatonin was carried out on the next day of drought treatment and was repeated weekly, while normal watering was regarded as control. Drought stress significantly enhanced oxidative stress markers and reduced growth parameters in water-deficit rose plants. However, melatonin spray (100 μM) produced increased plant height (16 %), flower yield (16 %), petal fresh and dry biomass (7 % and 38 %), total chlorophyll (48 %), contents of carotenoid (54 %), and gaseous exchange traits such as stomatal conductance (25 %), photosynthetic rate (91 %), and transpiration rate (3 %), in water-deficient plants. Likewise, the accretion of catalase, superoxide dismutase, soluble protein, proline, and glycine betaine contents was recorded by 22 %, 45 %, 58 %, 7 %, and 6 %, respectively, in drought-stressed plants, due to melatonin treatment. Increment of oxidative stress indicators i.e. malondialdehyde (−37 %) and hydrogen peroxide (−27 %) was diminished by melatonin triggered by drought stress. Furthermore, leaf cortex (51 %), vascular bundle area (76 %), palisade cell area (59 %), and lamina thickness (42 %) were remarkably increased with melatonin foliar sprays in water-deficit plants. The results of this study recommend that melatonin is a protective agent against drought stress and has potential application prospects in the rose-producing regions suffering from water deficiency. Future studies should focus on molecular responses of R. centifolia to drought stress to further develop stress alleviation strategies in floricultural crops. |
| format | Article |
| id | doaj-art-7ab8a8387620445bb52fa764c469316e |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-7ab8a8387620445bb52fa764c469316e2025-01-17T04:50:36ZengElsevierHeliyon2405-84402025-01-01111e41236Melatonin induces drought stress tolerance by regulating the physiological mechanisms, antioxidant enzymes, and leaf structural modifications in Rosa centifolia LMuhammad Ahsan0Adnan Younis1Aftab Jamal2Mohammed O. Alshaharni3Uthman Balgith Algopishi4Abeer Al-Andal5Mateen Sajid6Muhammad Naeem7Jawad Ahmad Khan8Emanuele Radicetti9Mohammad Valipour10Gulzar Akhtar11Department of Horticultural Sciences, The Islamia University of Bahawalpur, Bahawalpur, 63100, Pakistan; Corresponding author.Institute of Horticultural Sciences, University of Agriculture, Faisalabad, 38040, PakistanDepartment of Soil and Environmental Sciences, Faculty of Crop Production Sciences, The University of Agriculture, Peshawar, 25130, PakistanBiology Department, College of Science, King Khalid University, Abha, 61321, Saudi ArabiaDepartment of Biology, College of Science, King Khalid University, Abha, 61413, Saudi ArabiaDepartment of Biology, College of Science, King Khalid University, Abha, 61413, Saudi ArabiaDepartment of Horticulture, Ghazi University, Dera Ghazi Khan, 32200, PakistanDepartment of Pharmacy, Shah Abdul Latif University Khairpur, 66111, PakistanDepartment of Pharmacy, Shah Abdul Latif University Khairpur, 66111, PakistanDepartment of Chemical, Pharmaceutical and Agricultural Sciences (DOCPAS), University of Ferrara, 44121, Ferrara, Italy; Corresponding author.Department of Engineering and Engineering Technology, Metropolitan State University of Denver, Denver, CO, 80217, USADepartment of Horticulture, Muhammad Nawaz Shareef University of Agriculture, Multan, 66000, PakistanMelatonin is considered an effective bio-stimulant that is crucial in managing several abiotic stresses including drought. However, its potential mechanisms against drought stress in fragrant roses are not well understood. Here, we aim to investigate the role of melatonin on Rosa centifolia plants cultivated under drought stress (40 % field capacity) and normal irrigation (80 % field capacity). Plant growth traits, gaseous exchange, antioxidants, osmolytes, oxidative stress, and leaf anatomical attributes were measured. All pots were arranged with a completely randomized design with two-factor factorial setup. Foliar application of melatonin was carried out on the next day of drought treatment and was repeated weekly, while normal watering was regarded as control. Drought stress significantly enhanced oxidative stress markers and reduced growth parameters in water-deficit rose plants. However, melatonin spray (100 μM) produced increased plant height (16 %), flower yield (16 %), petal fresh and dry biomass (7 % and 38 %), total chlorophyll (48 %), contents of carotenoid (54 %), and gaseous exchange traits such as stomatal conductance (25 %), photosynthetic rate (91 %), and transpiration rate (3 %), in water-deficient plants. Likewise, the accretion of catalase, superoxide dismutase, soluble protein, proline, and glycine betaine contents was recorded by 22 %, 45 %, 58 %, 7 %, and 6 %, respectively, in drought-stressed plants, due to melatonin treatment. Increment of oxidative stress indicators i.e. malondialdehyde (−37 %) and hydrogen peroxide (−27 %) was diminished by melatonin triggered by drought stress. Furthermore, leaf cortex (51 %), vascular bundle area (76 %), palisade cell area (59 %), and lamina thickness (42 %) were remarkably increased with melatonin foliar sprays in water-deficit plants. The results of this study recommend that melatonin is a protective agent against drought stress and has potential application prospects in the rose-producing regions suffering from water deficiency. Future studies should focus on molecular responses of R. centifolia to drought stress to further develop stress alleviation strategies in floricultural crops.http://www.sciencedirect.com/science/article/pii/S2405844024172672Enzyme activityPhotosynthesisProlineSustainable floricultureWater deficiency |
| spellingShingle | Muhammad Ahsan Adnan Younis Aftab Jamal Mohammed O. Alshaharni Uthman Balgith Algopishi Abeer Al-Andal Mateen Sajid Muhammad Naeem Jawad Ahmad Khan Emanuele Radicetti Mohammad Valipour Gulzar Akhtar Melatonin induces drought stress tolerance by regulating the physiological mechanisms, antioxidant enzymes, and leaf structural modifications in Rosa centifolia L Heliyon Enzyme activity Photosynthesis Proline Sustainable floriculture Water deficiency |
| title | Melatonin induces drought stress tolerance by regulating the physiological mechanisms, antioxidant enzymes, and leaf structural modifications in Rosa centifolia L |
| title_full | Melatonin induces drought stress tolerance by regulating the physiological mechanisms, antioxidant enzymes, and leaf structural modifications in Rosa centifolia L |
| title_fullStr | Melatonin induces drought stress tolerance by regulating the physiological mechanisms, antioxidant enzymes, and leaf structural modifications in Rosa centifolia L |
| title_full_unstemmed | Melatonin induces drought stress tolerance by regulating the physiological mechanisms, antioxidant enzymes, and leaf structural modifications in Rosa centifolia L |
| title_short | Melatonin induces drought stress tolerance by regulating the physiological mechanisms, antioxidant enzymes, and leaf structural modifications in Rosa centifolia L |
| title_sort | melatonin induces drought stress tolerance by regulating the physiological mechanisms antioxidant enzymes and leaf structural modifications in rosa centifolia l |
| topic | Enzyme activity Photosynthesis Proline Sustainable floriculture Water deficiency |
| url | http://www.sciencedirect.com/science/article/pii/S2405844024172672 |
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