Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant <i>Saccharomycodes ludwigii</i> APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate
The presence of various inhibitory compounds in lignocellulosic hydrolysates poses a significant challenge for bioethanol production, requiring yeasts with exceptional multistress tolerance. This study introduces the novel application and demonstrates the robust performance of the nonconventional ye...
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2025-06-01
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| Series: | Energies |
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| Online Access: | https://www.mdpi.com/1996-1073/18/13/3428 |
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| author | Preekamol Klanrit Sudarat Thanonkeo Warayutt Pilap Jirawan Apiraksakorn Khanittha Fiala Ratanaporn Leesing Mamoru Yamada Pornthap Thanonkeo |
| author_facet | Preekamol Klanrit Sudarat Thanonkeo Warayutt Pilap Jirawan Apiraksakorn Khanittha Fiala Ratanaporn Leesing Mamoru Yamada Pornthap Thanonkeo |
| author_sort | Preekamol Klanrit |
| collection | DOAJ |
| description | The presence of various inhibitory compounds in lignocellulosic hydrolysates poses a significant challenge for bioethanol production, requiring yeasts with exceptional multistress tolerance. This study introduces the novel application and demonstrates the robust performance of the nonconventional yeast <i>Saccharomycodes ludwigii</i> APRE2 for efficient bioethanol production directly from undetoxified sugarcane bagasse hydrolysate (SBH) at 37 °C. This approach critically eliminates the need for the costly detoxification pretreatments often required in industrial processes. Initial experiments confirmed <i>S. ludwigii</i> APRE2’s capability to ferment undetoxified SBH. To optimize fermentation efficiency, a central composite design (CCD) approach was implemented. This statistical method identified the following precise optimal parameters: sugar concentration (143.95 g/L), diammonium phosphate (4.99 g/L), pH (4.98), yeast extract (8.94 g/L), and magnesium sulfate (2.22 g/L). Under these optimized conditions, impressive results were achieved: a maximum ethanol concentration of 38.11 g/L, productivity of 1.59 g/L·h, and yield of 0.45 g/g. Notably, the ethanol productivity and theoretical yield achieved by <i>S. ludwigii</i> APRE2 using this inhibitor-rich, undetoxified SBH (containing acetic acid, formic acid, furfural, and 5-(hydroxymethyl)furfural) were superior to those previously reported for other ethanologenic yeasts under similar challenging conditions. This research establishes <i>S. ludwigii</i> APRE2 as a highly promising and industrially viable candidate for sustainable bioethanol production from lignocellulosic biomass, with its key novelty being its superior performance on undetoxified feedstocks, potentially reducing overall production costs. |
| format | Article |
| id | doaj-art-7a8429a56c9c4491b0c8478d274dc5d3 |
| institution | Kabale University |
| issn | 1996-1073 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Energies |
| spelling | doaj-art-7a8429a56c9c4491b0c8478d274dc5d32025-08-20T03:50:16ZengMDPI AGEnergies1996-10732025-06-011813342810.3390/en18133428Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant <i>Saccharomycodes ludwigii</i> APRE2 Using Undetoxified Sugarcane Bagasse HydrolysatePreekamol Klanrit0Sudarat Thanonkeo1Warayutt Pilap2Jirawan Apiraksakorn3Khanittha Fiala4Ratanaporn Leesing5Mamoru Yamada6Pornthap Thanonkeo7Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandWalai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, ThailandWalai Rukhavej Botanical Research Institute, Mahasarakham University, Maha Sarakham 44150, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Microbiology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, ThailandDepartment of Biological Chemistry, Faculty of Agriculture, Yamaguchi University, Yamaguchi 753-8515, JapanDepartment of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002, ThailandThe presence of various inhibitory compounds in lignocellulosic hydrolysates poses a significant challenge for bioethanol production, requiring yeasts with exceptional multistress tolerance. This study introduces the novel application and demonstrates the robust performance of the nonconventional yeast <i>Saccharomycodes ludwigii</i> APRE2 for efficient bioethanol production directly from undetoxified sugarcane bagasse hydrolysate (SBH) at 37 °C. This approach critically eliminates the need for the costly detoxification pretreatments often required in industrial processes. Initial experiments confirmed <i>S. ludwigii</i> APRE2’s capability to ferment undetoxified SBH. To optimize fermentation efficiency, a central composite design (CCD) approach was implemented. This statistical method identified the following precise optimal parameters: sugar concentration (143.95 g/L), diammonium phosphate (4.99 g/L), pH (4.98), yeast extract (8.94 g/L), and magnesium sulfate (2.22 g/L). Under these optimized conditions, impressive results were achieved: a maximum ethanol concentration of 38.11 g/L, productivity of 1.59 g/L·h, and yield of 0.45 g/g. Notably, the ethanol productivity and theoretical yield achieved by <i>S. ludwigii</i> APRE2 using this inhibitor-rich, undetoxified SBH (containing acetic acid, formic acid, furfural, and 5-(hydroxymethyl)furfural) were superior to those previously reported for other ethanologenic yeasts under similar challenging conditions. This research establishes <i>S. ludwigii</i> APRE2 as a highly promising and industrially viable candidate for sustainable bioethanol production from lignocellulosic biomass, with its key novelty being its superior performance on undetoxified feedstocks, potentially reducing overall production costs.https://www.mdpi.com/1996-1073/18/13/3428biofuellignocellulosic materialsmultistress-tolerant yeastnonconventional yeast<i>Sacharomycodes ludwigii</i>sugarcane bagasse |
| spellingShingle | Preekamol Klanrit Sudarat Thanonkeo Warayutt Pilap Jirawan Apiraksakorn Khanittha Fiala Ratanaporn Leesing Mamoru Yamada Pornthap Thanonkeo Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant <i>Saccharomycodes ludwigii</i> APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate Energies biofuel lignocellulosic materials multistress-tolerant yeast nonconventional yeast <i>Sacharomycodes ludwigii</i> sugarcane bagasse |
| title | Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant <i>Saccharomycodes ludwigii</i> APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate |
| title_full | Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant <i>Saccharomycodes ludwigii</i> APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate |
| title_fullStr | Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant <i>Saccharomycodes ludwigii</i> APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate |
| title_full_unstemmed | Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant <i>Saccharomycodes ludwigii</i> APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate |
| title_short | Optimization of Fermentation Parameters for Enhanced Bioethanol Production by Multistress-Tolerant <i>Saccharomycodes ludwigii</i> APRE2 Using Undetoxified Sugarcane Bagasse Hydrolysate |
| title_sort | optimization of fermentation parameters for enhanced bioethanol production by multistress tolerant i saccharomycodes ludwigii i apre2 using undetoxified sugarcane bagasse hydrolysate |
| topic | biofuel lignocellulosic materials multistress-tolerant yeast nonconventional yeast <i>Sacharomycodes ludwigii</i> sugarcane bagasse |
| url | https://www.mdpi.com/1996-1073/18/13/3428 |
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