Defining the role of 2,2’,4,4’-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis
This study aims to investigates the effect of 2,2’,4,4’-tetrabromodiphenyl ether (BDE-47) on the differentiation of 3T3-L1 cells and its mechanism of action. These 3T3-L1 cells were induced to differentiate in vitro using methylisobutylxanthine, dexamethasone, and insulin conditions, then exposed to...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2024-12-01
|
| Series: | Adipocyte |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/21623945.2024.2430717 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1846136253687267328 |
|---|---|
| author | Zao-Ling Liu Aerna Qiayimaerdan Yong Fan Shu-Rui Jiang Zunire Tuerxuna Meng-Lin Wang Haiqiemuhan Abudureheman |
| author_facet | Zao-Ling Liu Aerna Qiayimaerdan Yong Fan Shu-Rui Jiang Zunire Tuerxuna Meng-Lin Wang Haiqiemuhan Abudureheman |
| author_sort | Zao-Ling Liu |
| collection | DOAJ |
| description | This study aims to investigates the effect of 2,2’,4,4’-tetrabromodiphenyl ether (BDE-47) on the differentiation of 3T3-L1 cells and its mechanism of action. These 3T3-L1 cells were induced to differentiate in vitro using methylisobutylxanthine, dexamethasone, and insulin conditions, then exposed to either 1% DMSO as a control group or varying concentrations of BDE-47 (2.5 μM, 7.5 μM, 12.5 μM, 18.75 μM, and 25 μM). Oil red O staining showed that the absorbance value of the BDE-47 exposure groups was higher than that of the control group (p < 0.05). This study identified 722 common genes between the differentially expressed genes of each exposure group. Using Cytoscape 10 hub genes were identified as Actb, Cdk1, Myc, Ccnb1, Aurkb, Plk1, Aurka, Pparg, Kif11, and Casp3. Enrichment analysis data revealed that the effects of BDE-47 on 3T3-L1 cell differentiation were associated with the cell cycle, p53 signalling, and PPARγ pathways. The transcription factor genes, KAT2A, MAX, SIN3A, TBP, and EP300, were shown to be associated with the PPARγ pathway. The mRNA expression of PPARγ in each exposure group was higher than that in the control group (p < 0.05), and a bimodal distribution between PPARγ mRNA expression and BDE-47 dose was observed. These findings indicate that BDE-47 May activate the PPARγ pathway and mitotic pathway to regulate the cell cycle and induce adipocyte differentiation. |
| format | Article |
| id | doaj-art-7a754217dcf94b6fbe30eb9893c2fded |
| institution | Kabale University |
| issn | 2162-3945 2162-397X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Adipocyte |
| spelling | doaj-art-7a754217dcf94b6fbe30eb9893c2fded2024-12-09T07:23:11ZengTaylor & Francis GroupAdipocyte2162-39452162-397X2024-12-0113110.1080/21623945.2024.2430717Defining the role of 2,2’,4,4’-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysisZao-Ling Liu0Aerna Qiayimaerdan1Yong Fan2Shu-Rui Jiang3Zunire Tuerxuna4Meng-Lin Wang5Haiqiemuhan Abudureheman6Department of Epidemiology & Health Statistics, School of public health, Xinjiang Medical University, Urumqi, Xinjiang, ChinaDepartment of Epidemiology & Health Statistics, School of public health, Xinjiang Medical University, Urumqi, Xinjiang, ChinaDepartment of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi, ChinaDepartment of Epidemiology & Health Statistics, School of public health, Xinjiang Medical University, Urumqi, Xinjiang, ChinaDepartment of Epidemiology & Health Statistics, School of public health, Xinjiang Medical University, Urumqi, Xinjiang, ChinaDepartment of Epidemiology & Health Statistics, School of public health, Xinjiang Medical University, Urumqi, Xinjiang, ChinaDepartment of Epidemiology & Health Statistics, School of public health, Xinjiang Medical University, Urumqi, Xinjiang, ChinaThis study aims to investigates the effect of 2,2’,4,4’-tetrabromodiphenyl ether (BDE-47) on the differentiation of 3T3-L1 cells and its mechanism of action. These 3T3-L1 cells were induced to differentiate in vitro using methylisobutylxanthine, dexamethasone, and insulin conditions, then exposed to either 1% DMSO as a control group or varying concentrations of BDE-47 (2.5 μM, 7.5 μM, 12.5 μM, 18.75 μM, and 25 μM). Oil red O staining showed that the absorbance value of the BDE-47 exposure groups was higher than that of the control group (p < 0.05). This study identified 722 common genes between the differentially expressed genes of each exposure group. Using Cytoscape 10 hub genes were identified as Actb, Cdk1, Myc, Ccnb1, Aurkb, Plk1, Aurka, Pparg, Kif11, and Casp3. Enrichment analysis data revealed that the effects of BDE-47 on 3T3-L1 cell differentiation were associated with the cell cycle, p53 signalling, and PPARγ pathways. The transcription factor genes, KAT2A, MAX, SIN3A, TBP, and EP300, were shown to be associated with the PPARγ pathway. The mRNA expression of PPARγ in each exposure group was higher than that in the control group (p < 0.05), and a bimodal distribution between PPARγ mRNA expression and BDE-47 dose was observed. These findings indicate that BDE-47 May activate the PPARγ pathway and mitotic pathway to regulate the cell cycle and induce adipocyte differentiation.https://www.tandfonline.com/doi/10.1080/21623945.2024.24307173T3-L1BDE-47adipogenesistranscriptome sequencingmitosis |
| spellingShingle | Zao-Ling Liu Aerna Qiayimaerdan Yong Fan Shu-Rui Jiang Zunire Tuerxuna Meng-Lin Wang Haiqiemuhan Abudureheman Defining the role of 2,2’,4,4’-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis Adipocyte 3T3-L1 BDE-47 adipogenesis transcriptome sequencing mitosis |
| title | Defining the role of 2,2’,4,4’-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis |
| title_full | Defining the role of 2,2’,4,4’-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis |
| title_fullStr | Defining the role of 2,2’,4,4’-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis |
| title_full_unstemmed | Defining the role of 2,2’,4,4’-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis |
| title_short | Defining the role of 2,2’,4,4’-tetrabromodiphenyl ether in 3T3-L1 cellular differentiation by transcriptome sequencing analysis |
| title_sort | defining the role of 2 2 4 4 tetrabromodiphenyl ether in 3t3 l1 cellular differentiation by transcriptome sequencing analysis |
| topic | 3T3-L1 BDE-47 adipogenesis transcriptome sequencing mitosis |
| url | https://www.tandfonline.com/doi/10.1080/21623945.2024.2430717 |
| work_keys_str_mv | AT zaolingliu definingtheroleof2244tetrabromodiphenyletherin3t3l1cellulardifferentiationbytranscriptomesequencinganalysis AT aernaqiayimaerdan definingtheroleof2244tetrabromodiphenyletherin3t3l1cellulardifferentiationbytranscriptomesequencinganalysis AT yongfan definingtheroleof2244tetrabromodiphenyletherin3t3l1cellulardifferentiationbytranscriptomesequencinganalysis AT shuruijiang definingtheroleof2244tetrabromodiphenyletherin3t3l1cellulardifferentiationbytranscriptomesequencinganalysis AT zuniretuerxuna definingtheroleof2244tetrabromodiphenyletherin3t3l1cellulardifferentiationbytranscriptomesequencinganalysis AT menglinwang definingtheroleof2244tetrabromodiphenyletherin3t3l1cellulardifferentiationbytranscriptomesequencinganalysis AT haiqiemuhanabudureheman definingtheroleof2244tetrabromodiphenyletherin3t3l1cellulardifferentiationbytranscriptomesequencinganalysis |