Risk factor assessment and microbiome analysis in peritoneal dialysis-related peritonitis reveal etiological characteristics
BackgroundPeritoneal dialysis-related peritonitis (PDRP) is one of the most common complications of peritoneal dialysis (PD). Understanding the risk factors and etiological characteristics is indispensable for infection prevention and improving the outcome and life quality.MethodsA total of 70 PD pa...
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Frontiers Media S.A.
2024-11-01
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| Series: | Frontiers in Immunology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1443468/full |
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| author | Li Zhang Hongrui Zhang Sensen Su Ye Jia Chenyang Liang Yuan Fang Dengwei Hong Tianyu Li Fuzhe Ma |
| author_facet | Li Zhang Hongrui Zhang Sensen Su Ye Jia Chenyang Liang Yuan Fang Dengwei Hong Tianyu Li Fuzhe Ma |
| author_sort | Li Zhang |
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| description | BackgroundPeritoneal dialysis-related peritonitis (PDRP) is one of the most common complications of peritoneal dialysis (PD). Understanding the risk factors and etiological characteristics is indispensable for infection prevention and improving the outcome and life quality.MethodsA total of 70 PD patients were separated into the PDRP group (n=25) and the control group (n=45). Variables, including gender, age, body mass index, primary diseases, and history of basic diseases, in the two groups were analyzed to assess the risk factors of PDRP. Metagenomic next-generation sequencing (mNGS) and microbial culture were compared in detecting pathogenic microorganisms. Gut microbiota analysis was performed in 35 PDRP patients based on mNGS data.ResultsDialysis time and times of dialysate change were the risk factors of PDRP, and times of dialysate change was the independent risk factor of PDRP (p = 0.046). mNGS produced higher sensitivity (65.79%) than microbial culture (36.84%) in identifying pathogenic microorganisms. Staphylococcus aureus and Klebsiella pneumoniae (four cases) were the most frequent pathogens causing PDRP, followed by Staphylococcus capitis (three cases). β diversity of the gut microbiota was significantly different between patients with fewer times of dialysate change (≤4) and more (>5), as well as between patients with gram-positive (G+) bacterial and gram-negative (G−) bacterial infection.ConclusionThe dialysis time and times of dialysate changes not only are risk factors for peritonitis in PD patients but also stimulate significant changes in the gut microbiome structure in PDRP patients. These findings may provide a novel viewpoint for the management of patients with PDRP. |
| format | Article |
| id | doaj-art-7a2403f3ff384e46b04f99d43de7e7b5 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-7a2403f3ff384e46b04f99d43de7e7b52024-11-14T06:21:07ZengFrontiers Media S.A.Frontiers in Immunology1664-32242024-11-011510.3389/fimmu.2024.14434681443468Risk factor assessment and microbiome analysis in peritoneal dialysis-related peritonitis reveal etiological characteristicsLi Zhang0Hongrui Zhang1Sensen Su2Ye Jia3Chenyang Liang4Yuan Fang5Dengwei Hong6Tianyu Li7Fuzhe Ma8Department of Nephrology, The First Hospital of Jilin University, Changchun, ChinaDepartment of Nephrology, The First Hospital of Jilin University, Changchun, ChinaDepartment of Nephrology, The First Hospital of Jilin University, Changchun, ChinaDepartment of Nephrology, The First Hospital of Jilin University, Changchun, ChinaDepartment of Nephrology, The First Hospital of Jilin University, Changchun, ChinaGenoxor Medical Science and Technology Inc., Shanghai, ChinaGenoxor Medical Science and Technology Inc., Shanghai, ChinaGenoxor Medical Science and Technology Inc., Shanghai, ChinaDepartment of Nephrology, The First Hospital of Jilin University, Changchun, ChinaBackgroundPeritoneal dialysis-related peritonitis (PDRP) is one of the most common complications of peritoneal dialysis (PD). Understanding the risk factors and etiological characteristics is indispensable for infection prevention and improving the outcome and life quality.MethodsA total of 70 PD patients were separated into the PDRP group (n=25) and the control group (n=45). Variables, including gender, age, body mass index, primary diseases, and history of basic diseases, in the two groups were analyzed to assess the risk factors of PDRP. Metagenomic next-generation sequencing (mNGS) and microbial culture were compared in detecting pathogenic microorganisms. Gut microbiota analysis was performed in 35 PDRP patients based on mNGS data.ResultsDialysis time and times of dialysate change were the risk factors of PDRP, and times of dialysate change was the independent risk factor of PDRP (p = 0.046). mNGS produced higher sensitivity (65.79%) than microbial culture (36.84%) in identifying pathogenic microorganisms. Staphylococcus aureus and Klebsiella pneumoniae (four cases) were the most frequent pathogens causing PDRP, followed by Staphylococcus capitis (three cases). β diversity of the gut microbiota was significantly different between patients with fewer times of dialysate change (≤4) and more (>5), as well as between patients with gram-positive (G+) bacterial and gram-negative (G−) bacterial infection.ConclusionThe dialysis time and times of dialysate changes not only are risk factors for peritonitis in PD patients but also stimulate significant changes in the gut microbiome structure in PDRP patients. These findings may provide a novel viewpoint for the management of patients with PDRP.https://www.frontiersin.org/articles/10.3389/fimmu.2024.1443468/fullperitoneal dialysis-related peritonitisrisk factormNGSpathogenic identificationgut microbiome |
| spellingShingle | Li Zhang Hongrui Zhang Sensen Su Ye Jia Chenyang Liang Yuan Fang Dengwei Hong Tianyu Li Fuzhe Ma Risk factor assessment and microbiome analysis in peritoneal dialysis-related peritonitis reveal etiological characteristics Frontiers in Immunology peritoneal dialysis-related peritonitis risk factor mNGS pathogenic identification gut microbiome |
| title | Risk factor assessment and microbiome analysis in peritoneal dialysis-related peritonitis reveal etiological characteristics |
| title_full | Risk factor assessment and microbiome analysis in peritoneal dialysis-related peritonitis reveal etiological characteristics |
| title_fullStr | Risk factor assessment and microbiome analysis in peritoneal dialysis-related peritonitis reveal etiological characteristics |
| title_full_unstemmed | Risk factor assessment and microbiome analysis in peritoneal dialysis-related peritonitis reveal etiological characteristics |
| title_short | Risk factor assessment and microbiome analysis in peritoneal dialysis-related peritonitis reveal etiological characteristics |
| title_sort | risk factor assessment and microbiome analysis in peritoneal dialysis related peritonitis reveal etiological characteristics |
| topic | peritoneal dialysis-related peritonitis risk factor mNGS pathogenic identification gut microbiome |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2024.1443468/full |
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