Proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinoma
Abstract Immunoglobulin diversity encompasses B-cell receptor, T-cell receptor, and antibody diversity. Existing studies have focused more on the role of B-cell and T-cell receptor diversity in tumor immunity, while the role of antibody diversity is less understood. This study examined and compared...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-11-01
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| Series: | Scientific Reports |
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| Online Access: | https://doi.org/10.1038/s41598-024-78955-6 |
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| _version_ | 1846165333895806976 |
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| author | Xinfu Huang Lijuan Xiong Yang Zhang Xin Peng Hongping Ba Peng Yang |
| author_facet | Xinfu Huang Lijuan Xiong Yang Zhang Xin Peng Hongping Ba Peng Yang |
| author_sort | Xinfu Huang |
| collection | DOAJ |
| description | Abstract Immunoglobulin diversity encompasses B-cell receptor, T-cell receptor, and antibody diversity. Existing studies have focused more on the role of B-cell and T-cell receptor diversity in tumor immunity, while the role of antibody diversity is less understood. This study examined and compared the blood extracellular vesicles (EVs) of lung cancer patients and healthy individuals using proteomics and bioinformatics analyses. The results revealed that among the 270 identified proteins, those involved in defense mechanisms were the most abundant. Most of these were antibody subtypes, accounting for 50.00%. Similarly, of the 40 identified EVs differentially expressed proteins (DEPs), 29 were involved in defense mechanisms (72.50%), with a higher proportion being antibody subtypes (82.76%). Furthermore, 24 DEP antibody subtypes were implicated in 18 immune reaction-related signaling pathways. These findings suggest that human serum EVs contain a significant number of antibody subtypes, and the antibody subtypes from lung cancer serum EVs differ from those of healthy controls (HCs). The variations in antibody diversity may be closely associated with lung cancer tumor immunity. |
| format | Article |
| id | doaj-art-799f0f40f9bf4e4e99b2474a7ef5f189 |
| institution | Kabale University |
| issn | 2045-2322 |
| language | English |
| publishDate | 2024-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj-art-799f0f40f9bf4e4e99b2474a7ef5f1892024-11-17T12:23:57ZengNature PortfolioScientific Reports2045-23222024-11-0114111110.1038/s41598-024-78955-6Proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinomaXinfu Huang0Lijuan Xiong1Yang Zhang2Xin Peng3Hongping Ba4Peng Yang5The Second Affiliated Hospital, Guizhou University of Traditional Chinese MedicineThe Second Affiliated Hospital, Guizhou University of Traditional Chinese MedicineThe Second Affiliated Hospital, Guizhou University of Traditional Chinese MedicineThe Second Affiliated Hospital, Guizhou University of Traditional Chinese MedicineDepartment of Quality Evaluation, Wuhan Center for Clinical LaboratoryThe Second Affiliated Hospital, Guizhou University of Traditional Chinese MedicineAbstract Immunoglobulin diversity encompasses B-cell receptor, T-cell receptor, and antibody diversity. Existing studies have focused more on the role of B-cell and T-cell receptor diversity in tumor immunity, while the role of antibody diversity is less understood. This study examined and compared the blood extracellular vesicles (EVs) of lung cancer patients and healthy individuals using proteomics and bioinformatics analyses. The results revealed that among the 270 identified proteins, those involved in defense mechanisms were the most abundant. Most of these were antibody subtypes, accounting for 50.00%. Similarly, of the 40 identified EVs differentially expressed proteins (DEPs), 29 were involved in defense mechanisms (72.50%), with a higher proportion being antibody subtypes (82.76%). Furthermore, 24 DEP antibody subtypes were implicated in 18 immune reaction-related signaling pathways. These findings suggest that human serum EVs contain a significant number of antibody subtypes, and the antibody subtypes from lung cancer serum EVs differ from those of healthy controls (HCs). The variations in antibody diversity may be closely associated with lung cancer tumor immunity.https://doi.org/10.1038/s41598-024-78955-6Lung adenocarcinomaPlasma extracellular vesiclesProteomicsAntibody diversityTumor immune |
| spellingShingle | Xinfu Huang Lijuan Xiong Yang Zhang Xin Peng Hongping Ba Peng Yang Proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinoma Scientific Reports Lung adenocarcinoma Plasma extracellular vesicles Proteomics Antibody diversity Tumor immune |
| title | Proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinoma |
| title_full | Proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinoma |
| title_fullStr | Proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinoma |
| title_full_unstemmed | Proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinoma |
| title_short | Proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinoma |
| title_sort | proteomic profile of the antibody diversity in circulating extracellular vesicles of lung adenocarcinoma |
| topic | Lung adenocarcinoma Plasma extracellular vesicles Proteomics Antibody diversity Tumor immune |
| url | https://doi.org/10.1038/s41598-024-78955-6 |
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