Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in Children
Immune reactions are among the most serious complications observed after hematopoietic stem cell transplantation (HSCT) in children. Microarray technique allows for simultaneous assessment of expression of nearly all human genes. The objective of the study was to compare the whole genome expression...
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/2626081 |
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| author | Szymon Skoczen Miroslaw Bik-Multanowski Jacek J. Pietrzyk Agnieszka Grabowska Kamil Fijorek Wojciech Strojny Kinga Klus-Kwiecinska Walentyna Balwierz Maciej Siedlar |
| author_facet | Szymon Skoczen Miroslaw Bik-Multanowski Jacek J. Pietrzyk Agnieszka Grabowska Kamil Fijorek Wojciech Strojny Kinga Klus-Kwiecinska Walentyna Balwierz Maciej Siedlar |
| author_sort | Szymon Skoczen |
| collection | DOAJ |
| description | Immune reactions are among the most serious complications observed after hematopoietic stem cell transplantation (HSCT) in children. Microarray technique allows for simultaneous assessment of expression of nearly all human genes. The objective of the study was to compare the whole genome expression in children before and after HSCT. A total of 33 children referred for HSCT were enrolled in the study. In 70% of the patients HSCT was performed for the treatment of neoplasms. Blood samples were obtained before HSCT and six months after the procedure. Subsequently, the whole genome expression was assessed in leukocytes using GeneChip Human Gene 1.0 ST microarray. The analysis of genomic profiles before and after HSCT revealed altered expression of 124 genes. Pathway enrichment analysis revealed upregulation of five pathways after HSCT: allograft rejection, graft-versus-host disease, type I diabetes mellitus, autoimmune thyroid disease, and viral myocarditis. The activation of those pathways seems to be related to immune reactions commonly observed after HSCT. Our results contribute to better understanding of the genomic background of the immunologic complications of HSCT. |
| format | Article |
| id | doaj-art-797c477d21444a6b97862aa2ad227ae3 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-797c477d21444a6b97862aa2ad227ae32025-02-03T05:47:09ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/26260812626081Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in ChildrenSzymon Skoczen0Miroslaw Bik-Multanowski1Jacek J. Pietrzyk2Agnieszka Grabowska3Kamil Fijorek4Wojciech Strojny5Kinga Klus-Kwiecinska6Walentyna Balwierz7Maciej Siedlar8Department of Clinical Immunology, Chair of Clinical Immunology and Transplantation, Institute of Pediatrics, Jagiellonian University Medical College, Wielicka Street 265, 30-663 Krakow, PolandDepartment of Medical Genetics, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, PolandDepartment of Medical Genetics, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, PolandDepartment of Medical Genetics, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, PolandDepartment of Statistics, Cracow University of Economics, Rakowicka Street 27, 31-510 Krakow, PolandDepartment of Oncology and Hematology, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, PolandDepartment of Oncology and Hematology, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, PolandDepartment of Oncology and Hematology, Institute of Pediatrics, Jagiellonian University Medical College, Krakow, PolandDepartment of Clinical Immunology, Chair of Clinical Immunology and Transplantation, Institute of Pediatrics, Jagiellonian University Medical College, Wielicka Street 265, 30-663 Krakow, PolandImmune reactions are among the most serious complications observed after hematopoietic stem cell transplantation (HSCT) in children. Microarray technique allows for simultaneous assessment of expression of nearly all human genes. The objective of the study was to compare the whole genome expression in children before and after HSCT. A total of 33 children referred for HSCT were enrolled in the study. In 70% of the patients HSCT was performed for the treatment of neoplasms. Blood samples were obtained before HSCT and six months after the procedure. Subsequently, the whole genome expression was assessed in leukocytes using GeneChip Human Gene 1.0 ST microarray. The analysis of genomic profiles before and after HSCT revealed altered expression of 124 genes. Pathway enrichment analysis revealed upregulation of five pathways after HSCT: allograft rejection, graft-versus-host disease, type I diabetes mellitus, autoimmune thyroid disease, and viral myocarditis. The activation of those pathways seems to be related to immune reactions commonly observed after HSCT. Our results contribute to better understanding of the genomic background of the immunologic complications of HSCT.http://dx.doi.org/10.1155/2016/2626081 |
| spellingShingle | Szymon Skoczen Miroslaw Bik-Multanowski Jacek J. Pietrzyk Agnieszka Grabowska Kamil Fijorek Wojciech Strojny Kinga Klus-Kwiecinska Walentyna Balwierz Maciej Siedlar Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in Children Stem Cells International |
| title | Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in Children |
| title_full | Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in Children |
| title_fullStr | Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in Children |
| title_full_unstemmed | Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in Children |
| title_short | Genetic Background of Immune Complications after Allogeneic Hematopoietic Stem Cell Transplantation in Children |
| title_sort | genetic background of immune complications after allogeneic hematopoietic stem cell transplantation in children |
| url | http://dx.doi.org/10.1155/2016/2626081 |
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