ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVE

Despite availability of many antifungal agents, antifungal clinical resistance occurs, perhaps as a result of an infecting organism found to be resistant in vitro to one or more antifungals tested. Thus, antifungal susceptibility testing (AFST) results, if timely generated by the clinical microbiolo...

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Main Authors: Brunella Posteraro, Riccardo Torelli, Elena De Carolis, Patrizia Posteraro, Maurizio Sanguinetti
Format: Article
Language:English
Published: PAGEPress Publications 2014-04-01
Series:Mediterranean Journal of Hematology and Infectious Diseases
Subjects:
Online Access:http://www.mjhid.org/index.php/mjhid/article/view/1367
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author Brunella Posteraro
Riccardo Torelli
Elena De Carolis
Patrizia Posteraro
Maurizio Sanguinetti
author_facet Brunella Posteraro
Riccardo Torelli
Elena De Carolis
Patrizia Posteraro
Maurizio Sanguinetti
author_sort Brunella Posteraro
collection DOAJ
description Despite availability of many antifungal agents, antifungal clinical resistance occurs, perhaps as a result of an infecting organism found to be resistant in vitro to one or more antifungals tested. Thus, antifungal susceptibility testing (AFST) results, if timely generated by the clinical microbiology and communicated to clinicians, can aid them in the therapeutic decision making, especially for difficult-to-treat invasive candidiasis and aspergillosis. Although recently refined AFST methods are commercially available to allow a close antifungal resistance surveillance in many clinical setting, novel assays, relying on short-time antifungal drug exposure of fungal isolates, are upcoming tools for AFST. Based on emerging technologies such as flow cytometry, MALDI-TOF mass spectrometry, and isothermal microcalorimetry, these assays could provide a reliable means for quicker and sensitive assessment of AFST.
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id doaj-art-7975afd6e38b4bea8384c4dd9f864a8d
institution Kabale University
issn 2035-3006
language English
publishDate 2014-04-01
publisher PAGEPress Publications
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series Mediterranean Journal of Hematology and Infectious Diseases
spelling doaj-art-7975afd6e38b4bea8384c4dd9f864a8d2025-01-02T11:13:55ZengPAGEPress PublicationsMediterranean Journal of Hematology and Infectious Diseases2035-30062014-04-0161e2014030e201403010.4084/mjhid.2014.0301127ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVEBrunella Posteraro0Riccardo Torelli1Elena De Carolis2Patrizia Posteraro3Maurizio Sanguinetti4Catholic University. Public HealthCatholic University. MicrobiologyCatholic University. MicrobiologyClinical Laboratory, Ospedale San Carlo, RomeCatholic University. MicrobiologyDespite availability of many antifungal agents, antifungal clinical resistance occurs, perhaps as a result of an infecting organism found to be resistant in vitro to one or more antifungals tested. Thus, antifungal susceptibility testing (AFST) results, if timely generated by the clinical microbiology and communicated to clinicians, can aid them in the therapeutic decision making, especially for difficult-to-treat invasive candidiasis and aspergillosis. Although recently refined AFST methods are commercially available to allow a close antifungal resistance surveillance in many clinical setting, novel assays, relying on short-time antifungal drug exposure of fungal isolates, are upcoming tools for AFST. Based on emerging technologies such as flow cytometry, MALDI-TOF mass spectrometry, and isothermal microcalorimetry, these assays could provide a reliable means for quicker and sensitive assessment of AFST.http://www.mjhid.org/index.php/mjhid/article/view/1367Fungal infections, Antifungal agentsSusceptibility testing
spellingShingle Brunella Posteraro
Riccardo Torelli
Elena De Carolis
Patrizia Posteraro
Maurizio Sanguinetti
ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVE
Mediterranean Journal of Hematology and Infectious Diseases
Fungal infections, Antifungal agents
Susceptibility testing
title ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVE
title_full ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVE
title_fullStr ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVE
title_full_unstemmed ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVE
title_short ANTIFUNGAL SUSCEPTIBILITY TESTING: CURRENT ROLE FROM THE CLINICAL LABORATORY PERSPECTIVE
title_sort antifungal susceptibility testing current role from the clinical laboratory perspective
topic Fungal infections, Antifungal agents
Susceptibility testing
url http://www.mjhid.org/index.php/mjhid/article/view/1367
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AT elenadecarolis antifungalsusceptibilitytestingcurrentrolefromtheclinicallaboratoryperspective
AT patriziaposteraro antifungalsusceptibilitytestingcurrentrolefromtheclinicallaboratoryperspective
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