Maximizing Biomass Production and Carotenoid-like Pigments Yield in <i>Kocuria sediminis</i> As04 Through Culture Optimization
The global chemical pigment industry faces environmental challenges despite its economic importance. This study investigates the potential of <i>Kocuria sediminis</i> AS04, an airborne isolate, for sustainable pigment and biomass production. Microbial kinetics were evaluated under Taguch...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/7/1555 |
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| Summary: | The global chemical pigment industry faces environmental challenges despite its economic importance. This study investigates the potential of <i>Kocuria sediminis</i> AS04, an airborne isolate, for sustainable pigment and biomass production. Microbial kinetics were evaluated under Taguchi design conditions with temperature (30, 34, and 38 °C), stirring speed (110, 120, and 130), and pH (6.0, 6.5, and 7.0), measuring biomass through dry weight and viable cells, pigment production, and identification of its pigment using UPLC-MS/MS; structural and chemical characterization of biomass was conducted using SEM and FTIR. Among the tested conditions, the treatment at 30 °C, 130 rpm, and pH 6.5 resulted in the highest CFU count (5.7 × 10<sup>9</sup> CFU mL<sup>−1</sup>) and the greatest biomass yield (13.3 g L<sup>−1</sup>). In contrast, the highest pigment yield (0.0016 mg g<sup>−1</sup>) was obtained at 38 °C, 130 rpm, and pH 6.0. Cell extracts identified key carotenoid compounds such as β-cryptoxanthin, Rhodovibrin, and other precursors. These findings highlight the potential of <i>Kocuria sediminis</i> AS04 as a sustainable source of pigments and valuable bioproducts, offering promising alternatives for eco-friendly industrial applications. |
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| ISSN: | 2076-2607 |