低能量氦氖激光照射促进体外培养神经元的突起生长和RNA合成

机械分离新生大白鼠颈上节(superior cervical ganglia)进行分离细胞培养。培养物分为激光照射组与对照组,两组均在含有神经生长因子(nerve growth factor)的培养液中生长。激光照射组每隔2小时用4毫瓦氦氖激光(Helium-Neon laser)照射一次,每次5分钟,直至终止培养。对照组未经激光照射。两组培养物分别培养20、22、24和28小时,测量培养物的神经元突起(neurite)生长;在培养22小时,用~3H-尿嘧啶核苷和~3H-胸腺嘧啶核苷标记培养物的细胞,放射自显影法观察神经元的DNA和RNA合成能力。实验结果表明,低能量氦氖激光照射能促进体外培养...

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Bibliographic Details
Main Author:	周汉城
Format:	Article
Language:	zho
Published:	Editorial Office of Journal of Sun Yat-sen University 1988-01-01
Series:	Zhongshan Daxue xuebao. Yixue kexue ban
Subjects:	'分离细胞培养' ">分离细胞培养 '氦氖激光' ">氦氖激光 '神经突起' ">神经突起 '神经生长因子(NGF)' ">神经生长因子(NGF) &nbsp cursor: pointer text-decoration: none white-space: normal word-spacing: 0px text-transform: none font-weight: 400 color: rgb(102 102 102) outline-width: medium padding-bottom: 0px font-style: normal text-align: justify padding-top: 0px outline-style: none padding-left: 0px orphans: 2 widows: 2 margin: 0px letter-spacing: normal outline-color: invert padding-right: 0px background-color: rgb(255 255 255) text-indent: 0px font-variant-ligatures: normal font-variant-caps: normal -webkit-text-stroke-width: 0px" onclick="'#10 TurnPageToKnet('kw' '核糖核酸(RNA)' '')'#10 ">核糖核酸(RNA)
Online Access:	http://xuebaoyx.sysu.edu.cn/zh/article/43593879/
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低能量氦氖激光照射促进体外培养神经元的突起生长和RNA合成

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