低能量氦氖激光照射促进体外培养神经元的突起生长和RNA合成

低能量氦氖激光照射促进体外培养神经元的突起生长和RNA合成

机械分离新生大白鼠颈上节(superior cervical ganglia)进行分离细胞培养。培养物分为激光照射组与对照组,两组均在含有神经生长因子(nerve growth factor)的培养液中生长。激光照射组每隔2小时用4毫瓦氦氖激光(Helium-Neon laser)照射一次,每次5分钟,直至终止培养。对照组未经激光照射。两组培养物分别培养20、22、24和28小时,测量培养物的神经元突起(neurite)生长;在培养22小时,用~3H-尿嘧啶核苷和~3H-胸腺嘧啶核苷标记培养物的细胞,放射自显影法观察神经元的DNA和RNA合成能力。实验结果表明,低能量氦氖激光照射能促进体外培养...

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Bibliographic Details
Main Author: 周汉城
Format: Article
Language:zho
Published: Editorial Office of Journal of Sun Yat-sen University 1988-01-01
Series:Zhongshan Daxue xuebao. Yixue kexue ban
Subjects:
'分离细胞培养'
">分离细胞培养
'氦氖激光'
">氦氖激光
'神经突起'
">神经突起
'神经生长因子(NGF)'
">神经生长因子(NGF)
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'核糖核酸(RNA)'
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">核糖核酸(RNA)
Online Access:http://xuebaoyx.sysu.edu.cn/zh/article/43593879/
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