Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of <i>qMLWR11.1</i> and <i>qMLWR11.2</i>
Grain shape in rice (<i>Oryza sativa</i> L.) is a complex trait governed by multiple quantitative trait loci (QTLs). To dissect the genetic basis of rice shape, QTL analysis was conducted for milled rice grain width (MGW), milled rice grain length (MGL), and milled rice length-to-width r...
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2024-11-01
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| author | Liting Zhang Zhanhua Lu Zhaoyang Pan Tengkui Chen Shiguang Wang Wei Liu Xiaofei Wang Haoxiang Wu Hao Chen Yunyi Zhan Xiuying He |
| author_facet | Liting Zhang Zhanhua Lu Zhaoyang Pan Tengkui Chen Shiguang Wang Wei Liu Xiaofei Wang Haoxiang Wu Hao Chen Yunyi Zhan Xiuying He |
| author_sort | Liting Zhang |
| collection | DOAJ |
| description | Grain shape in rice (<i>Oryza sativa</i> L.) is a complex trait governed by multiple quantitative trait loci (QTLs). To dissect the genetic basis of rice shape, QTL analysis was conducted for milled rice grain width (MGW), milled rice grain length (MGL), and milled rice length-to-width ratio (MLWR) using a recombinant inbred line (RIL) population of F<sub>10</sub> and F<sub>11</sub> generations derived from a cross between Yuexiangzhan and Shengbasimiao. A high-density genetic map consisting of 2412 bins was constructed by sequencing 184 RILs, spanning a total length of 2376.46 cM. A total of 19 QTLs related to MGL, MGW, and MLWR were detected under two environments. The range of phenotypic variation attributed to individual QTL ranged from 1.67% to 32.08%. Among those, a novel locus for MGL, MGW and MLWR, designated as <i>qMLWR3.2</i>, was pinpointed within a specific ~0.96-Mb region. Two novel loci for MGW and MLWR, <i>qMLWR11.1</i> and <i>qMLWR11.2</i>, were verified within ~1.22-Mb and ~0.52-Mb regions using three RIL-developed populations, respectively. These findings lay the foundation for further map-based cloning and molecular design breeding in rice. |
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| institution | Kabale University |
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| publishDate | 2024-11-01 |
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| spelling | doaj-art-786d23fb6d5f4325bbd3d498313f563e2024-11-26T18:18:49ZengMDPI AGPlants2223-77472024-11-011322317810.3390/plants13223178Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of <i>qMLWR11.1</i> and <i>qMLWR11.2</i>Liting Zhang0Zhanhua Lu1Zhaoyang Pan2Tengkui Chen3Shiguang Wang4Wei Liu5Xiaofei Wang6Haoxiang Wu7Hao Chen8Yunyi Zhan9Xiuying He10Rice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaRice Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, ChinaGrain shape in rice (<i>Oryza sativa</i> L.) is a complex trait governed by multiple quantitative trait loci (QTLs). To dissect the genetic basis of rice shape, QTL analysis was conducted for milled rice grain width (MGW), milled rice grain length (MGL), and milled rice length-to-width ratio (MLWR) using a recombinant inbred line (RIL) population of F<sub>10</sub> and F<sub>11</sub> generations derived from a cross between Yuexiangzhan and Shengbasimiao. A high-density genetic map consisting of 2412 bins was constructed by sequencing 184 RILs, spanning a total length of 2376.46 cM. A total of 19 QTLs related to MGL, MGW, and MLWR were detected under two environments. The range of phenotypic variation attributed to individual QTL ranged from 1.67% to 32.08%. Among those, a novel locus for MGL, MGW and MLWR, designated as <i>qMLWR3.2</i>, was pinpointed within a specific ~0.96-Mb region. Two novel loci for MGW and MLWR, <i>qMLWR11.1</i> and <i>qMLWR11.2</i>, were verified within ~1.22-Mb and ~0.52-Mb regions using three RIL-developed populations, respectively. These findings lay the foundation for further map-based cloning and molecular design breeding in rice.https://www.mdpi.com/2223-7747/13/22/3178rice (<i>Oryza sativa</i> L.)appearance qualitymilled rice grain shapeMGLMGWMLWR |
| spellingShingle | Liting Zhang Zhanhua Lu Zhaoyang Pan Tengkui Chen Shiguang Wang Wei Liu Xiaofei Wang Haoxiang Wu Hao Chen Yunyi Zhan Xiuying He Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of <i>qMLWR11.1</i> and <i>qMLWR11.2</i> Plants rice (<i>Oryza sativa</i> L.) appearance quality milled rice grain shape MGL MGW MLWR |
| title | Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of <i>qMLWR11.1</i> and <i>qMLWR11.2</i> |
| title_full | Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of <i>qMLWR11.1</i> and <i>qMLWR11.2</i> |
| title_fullStr | Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of <i>qMLWR11.1</i> and <i>qMLWR11.2</i> |
| title_full_unstemmed | Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of <i>qMLWR11.1</i> and <i>qMLWR11.2</i> |
| title_short | Genetic Dissection of Milled Rice Grain Shape by Using a Recombinant Inbred Line Population and Validation of <i>qMLWR11.1</i> and <i>qMLWR11.2</i> |
| title_sort | genetic dissection of milled rice grain shape by using a recombinant inbred line population and validation of i qmlwr11 1 i and i qmlwr11 2 i |
| topic | rice (<i>Oryza sativa</i> L.) appearance quality milled rice grain shape MGL MGW MLWR |
| url | https://www.mdpi.com/2223-7747/13/22/3178 |
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