Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007
BACKGROUND: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) differ from health care-associated MRSA (HA-MRSA) in their genotypic and phenotypic characteristics. The purpose of the present study was to compare the demographics, antimicrobial susceptibilities and molecular e...
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| Format: | Article |
| Language: | English |
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Wiley
2009-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2009/853676 |
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| author | Kimberly A Nichol Melissa McCracken Melanie R DeCorby Kristjan Thompson Michael R Mulvey James A Karlowsky Daryl J Hoban George G Zhanel |
| author_facet | Kimberly A Nichol Melissa McCracken Melanie R DeCorby Kristjan Thompson Michael R Mulvey James A Karlowsky Daryl J Hoban George G Zhanel |
| author_sort | Kimberly A Nichol |
| collection | DOAJ |
| description | BACKGROUND: Community-associated methicillin-resistant
Staphylococcus aureus (CA-MRSA) differ from health care-associated
MRSA (HA-MRSA) in their genotypic and phenotypic characteristics.
The purpose of the present study was to compare the demographics,
antimicrobial susceptibilities and molecular epidemiology of
CA-MRSA and HA-MRSA in Canada.
METHODS: In 2007, 385 MRSA isolates were collected from
Canadian patients attending hospital clinics, emergency rooms, medical/
surgical wards and intensive care units. Susceptibilities to betalactams,
clarithromycin, clindamycin, daptomycin, levofloxacin,
linezolid, moxifloxacin, tigecycline, trimethoprim-sulfamethoxazole
and vancomycin were determined by Clinical and Laboratory
Standards Institute broth microdilution. Strain typing was performed
by pulsed-field gel electrophoresis (PFGE) and the mecA, nuc and pvl
genes were detected by polymerase chain reaction.
RESULTS: Of the 385 MRSA, 19.5% were CA-MRSA and 79.2%
were HA-MRSA as determined by PFGE. CA-MRSA belonged to
PFGE types CMRSA10/USA300 (66.7%) and CMRSA7/USA400
(33.3%); PFGE types identified among HA-MRSA included
CMRSA2/USA100/800 (81.6%), CMRSA6 (13.1%), CMRSA1/
USA600 (3.3%), CMRSA5/USA500 (1.3%), CMRSA3 (0.3%) and
CMRSA9 (0.3%). Panton-Valentine leukocidin (PVL) was detected
in 94.7% of CA-MRSA and 0.7% of HA-MRSA. Resistance rates
(CA-MRSA versus HA-MRSA) were 61.3% versus 97.7% to levofloxacin,
73.3% versus 96.7% to clarithromycin, 12.0% versus 74.8% to
clindamycin and 0.0% versus 15.4% to trimethoprim-sulfamethoxazole.
No MRSA were resistant to vancomycin, linezolid, tigecycline or
daptomycin.
CONCLUSIONS: CA-MRSA represented 19.5% of all MRSA.
CA-MRSA was significantly more susceptible to levofloxacin,
clarithromycin, clindamycin and trimethoprim-sulfamethoxazole than
HA-MRSA. Of CA-MRSA, 94.7% were PVL-positive while 99.3% of
HA-MRSA were PVL-negative. CA-MRSA is an emerging pathogen
in Canadian hospitals. |
| format | Article |
| id | doaj-art-786359bd9b31407ca50868827b28bdee |
| institution | Kabale University |
| issn | 1712-9532 |
| language | English |
| publishDate | 2009-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-786359bd9b31407ca50868827b28bdee2025-02-03T05:52:52ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95322009-01-0120Suppl A31A36A10.1155/2009/853676Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007Kimberly A Nichol0Melissa McCracken1Melanie R DeCorby2Kristjan Thompson3Michael R Mulvey4James A Karlowsky5Daryl J Hoban6George G Zhanel7Diagnostic Services of Manitoba∕Department of Clinical Microbiology, Health Sciences Centre, CanadaNational Microbiology Laboratory, , Public Health Agency of Canada, CanadaDepartment of Medical Microbiology and Infectious Diseases, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, CanadaDepartment of Medical Microbiology and Infectious Diseases, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, CanadaNational Microbiology Laboratory, , Public Health Agency of Canada, CanadaDiagnostic Services of Manitoba∕Department of Clinical Microbiology, Health Sciences Centre, CanadaDiagnostic Services of Manitoba∕Department of Clinical Microbiology, Health Sciences Centre, CanadaDepartment of Medical Microbiology and Infectious Diseases, Faculty of Medicine, University of Manitoba, Winnipeg, Manitoba, CanadaBACKGROUND: Community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) differ from health care-associated MRSA (HA-MRSA) in their genotypic and phenotypic characteristics. The purpose of the present study was to compare the demographics, antimicrobial susceptibilities and molecular epidemiology of CA-MRSA and HA-MRSA in Canada. METHODS: In 2007, 385 MRSA isolates were collected from Canadian patients attending hospital clinics, emergency rooms, medical/ surgical wards and intensive care units. Susceptibilities to betalactams, clarithromycin, clindamycin, daptomycin, levofloxacin, linezolid, moxifloxacin, tigecycline, trimethoprim-sulfamethoxazole and vancomycin were determined by Clinical and Laboratory Standards Institute broth microdilution. Strain typing was performed by pulsed-field gel electrophoresis (PFGE) and the mecA, nuc and pvl genes were detected by polymerase chain reaction. RESULTS: Of the 385 MRSA, 19.5% were CA-MRSA and 79.2% were HA-MRSA as determined by PFGE. CA-MRSA belonged to PFGE types CMRSA10/USA300 (66.7%) and CMRSA7/USA400 (33.3%); PFGE types identified among HA-MRSA included CMRSA2/USA100/800 (81.6%), CMRSA6 (13.1%), CMRSA1/ USA600 (3.3%), CMRSA5/USA500 (1.3%), CMRSA3 (0.3%) and CMRSA9 (0.3%). Panton-Valentine leukocidin (PVL) was detected in 94.7% of CA-MRSA and 0.7% of HA-MRSA. Resistance rates (CA-MRSA versus HA-MRSA) were 61.3% versus 97.7% to levofloxacin, 73.3% versus 96.7% to clarithromycin, 12.0% versus 74.8% to clindamycin and 0.0% versus 15.4% to trimethoprim-sulfamethoxazole. No MRSA were resistant to vancomycin, linezolid, tigecycline or daptomycin. CONCLUSIONS: CA-MRSA represented 19.5% of all MRSA. CA-MRSA was significantly more susceptible to levofloxacin, clarithromycin, clindamycin and trimethoprim-sulfamethoxazole than HA-MRSA. Of CA-MRSA, 94.7% were PVL-positive while 99.3% of HA-MRSA were PVL-negative. CA-MRSA is an emerging pathogen in Canadian hospitals.http://dx.doi.org/10.1155/2009/853676 |
| spellingShingle | Kimberly A Nichol Melissa McCracken Melanie R DeCorby Kristjan Thompson Michael R Mulvey James A Karlowsky Daryl J Hoban George G Zhanel Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007 Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007 |
| title_full | Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007 |
| title_fullStr | Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007 |
| title_full_unstemmed | Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007 |
| title_short | Comparison of Community-Associated and Health Care-Associated Methicillin-Resistant Staphylococcus aureus in Canada: Results from CANWARD 2007 |
| title_sort | comparison of community associated and health care associated methicillin resistant staphylococcus aureus in canada results from canward 2007 |
| url | http://dx.doi.org/10.1155/2009/853676 |
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