Effectiveness of ultrasound (US) and slightly acidic electrolyzed water (SAEW) treatments for removing Listeria monocytogenes biofilms
Biofilms can persist in food industry environments leading to repeated cross-contamination, thus threatening human health. Slightly acid electrolyzed water (SAEW) and ultrasound have emerged as environmentally-friendly antimicrobial agents. However, their bactericidal efficacy is not high when used...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-01-01
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| Series: | Ultrasonics Sonochemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1350417724004395 |
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| author | Hongrui Ren Yu Quan Shaokang Liu Jianxiong Hao |
| author_facet | Hongrui Ren Yu Quan Shaokang Liu Jianxiong Hao |
| author_sort | Hongrui Ren |
| collection | DOAJ |
| description | Biofilms can persist in food industry environments leading to repeated cross-contamination, thus threatening human health. Slightly acid electrolyzed water (SAEW) and ultrasound have emerged as environmentally-friendly antimicrobial agents. However, their bactericidal efficacy is not high when used alone. In the present study, the effect of ultrasound combined with SAEW on removing Listeria monocytogenes biofilms from a glass surface was evaluated. Listeria monocytogenes biofilms were treated by immersion in sterilized deionized water (control treatment), immersion in sterilized deionized water combined with ultrasound (US treatment), immersion in SAEW (SAEW treatment), immersion in SAEW combined with ultrasound treatment (SAEW + US treatment), immersion in SAEW followed by immersion in sterilized deionized water combined with ultrasound (SAEW − US treatment), and immersion in sterilized deionized water combined with ultrasound followed by immersion in SAEW (US − SAEW treatment). The results showed that treating biofilms by US − SAEW treatment led to the lowest number of Listeria monocytogenes, the lowest amount of biofilm remaining on the glass slide were visualized by crystal violet staining and scanning electron microscope, and a minimal content of extracellular polymeric substances. Overall, the highest biofilm removal efficacy was observed for the US − SAEW treatment. |
| format | Article |
| id | doaj-art-7818ca176df54736b2613c25f644cc43 |
| institution | Kabale University |
| issn | 1350-4177 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ultrasonics Sonochemistry |
| spelling | doaj-art-7818ca176df54736b2613c25f644cc432025-01-11T06:38:47ZengElsevierUltrasonics Sonochemistry1350-41772025-01-01112107190Effectiveness of ultrasound (US) and slightly acidic electrolyzed water (SAEW) treatments for removing Listeria monocytogenes biofilmsHongrui Ren0Yu Quan1Shaokang Liu2Jianxiong Hao3College of Food Science and Biology, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang 050018, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang 050018, ChinaCollege of Food Science and Biology, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang 050018, ChinaCorresponding author.; College of Food Science and Biology, Hebei University of Science and Technology, No. 26 Yuxiang Street, Shijiazhuang 050018, ChinaBiofilms can persist in food industry environments leading to repeated cross-contamination, thus threatening human health. Slightly acid electrolyzed water (SAEW) and ultrasound have emerged as environmentally-friendly antimicrobial agents. However, their bactericidal efficacy is not high when used alone. In the present study, the effect of ultrasound combined with SAEW on removing Listeria monocytogenes biofilms from a glass surface was evaluated. Listeria monocytogenes biofilms were treated by immersion in sterilized deionized water (control treatment), immersion in sterilized deionized water combined with ultrasound (US treatment), immersion in SAEW (SAEW treatment), immersion in SAEW combined with ultrasound treatment (SAEW + US treatment), immersion in SAEW followed by immersion in sterilized deionized water combined with ultrasound (SAEW − US treatment), and immersion in sterilized deionized water combined with ultrasound followed by immersion in SAEW (US − SAEW treatment). The results showed that treating biofilms by US − SAEW treatment led to the lowest number of Listeria monocytogenes, the lowest amount of biofilm remaining on the glass slide were visualized by crystal violet staining and scanning electron microscope, and a minimal content of extracellular polymeric substances. Overall, the highest biofilm removal efficacy was observed for the US − SAEW treatment.http://www.sciencedirect.com/science/article/pii/S1350417724004395Listeria monocytogenesBiofilmSlightly acidic electrolyzed waterUltrasound |
| spellingShingle | Hongrui Ren Yu Quan Shaokang Liu Jianxiong Hao Effectiveness of ultrasound (US) and slightly acidic electrolyzed water (SAEW) treatments for removing Listeria monocytogenes biofilms Ultrasonics Sonochemistry Listeria monocytogenes Biofilm Slightly acidic electrolyzed water Ultrasound |
| title | Effectiveness of ultrasound (US) and slightly acidic electrolyzed water (SAEW) treatments for removing Listeria monocytogenes biofilms |
| title_full | Effectiveness of ultrasound (US) and slightly acidic electrolyzed water (SAEW) treatments for removing Listeria monocytogenes biofilms |
| title_fullStr | Effectiveness of ultrasound (US) and slightly acidic electrolyzed water (SAEW) treatments for removing Listeria monocytogenes biofilms |
| title_full_unstemmed | Effectiveness of ultrasound (US) and slightly acidic electrolyzed water (SAEW) treatments for removing Listeria monocytogenes biofilms |
| title_short | Effectiveness of ultrasound (US) and slightly acidic electrolyzed water (SAEW) treatments for removing Listeria monocytogenes biofilms |
| title_sort | effectiveness of ultrasound us and slightly acidic electrolyzed water saew treatments for removing listeria monocytogenes biofilms |
| topic | Listeria monocytogenes Biofilm Slightly acidic electrolyzed water Ultrasound |
| url | http://www.sciencedirect.com/science/article/pii/S1350417724004395 |
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